Nano-cuprous oxide enhances seed germination and seedling growth in Lycopersicum esculentum plants

  • Ananda S Sapthagiri college of Engineering
  • Shobha G
  • Shashidhara KS
  • Vishwaprakash Mahadimane

Abstract

This study was carried out to determine the influence of cuprous oxide nanoparticles (Cu2O NPs) biosynthesised from leaf extracts of Flacourtia montana on the tomato Lycioersicum esculentum seed germination, seedling growth and vigour index. Here we examined the promotory and phytotoxic effect of Cu2O NPs (0-160ppm) on tomato seeds resulted in dosage dependent response. The highest germination percentage (95%) was observed at 20ppm Cu2O NPs, however, above 20ppm Cu2O NPs, there is a reduction in the seed germination. The tomato seedlings showed increased root and shoot elongation up to 20ppm Cu2O NPs concentration, further increase in NPs concentration caused the negative effect on plants growth and development. The leaf pigments showed increasing trend in tomato plants after treatment with Cu2O NPs up to 20ppm as compared to control. Phytotoxicity of Cu2O NPs in tomato seedlings demonstrated by lower contents of chlorophyll a, b and carotenoid pigments. The study of effect on antioxidant enzymes showed increases in activity with increase in Cu2O NPs concentration for two enzymes, Super oxide dismutase (SOD) and Glutathione Peroxidase (GPX) out of five enzymes treated. High antioxidant activity of enzymes is followed by the increased lipid peroxidation and decrease in free radical scavenging activity by the DPPH. The activity of Catalase, Pheny Alanine Aminolyase and Poly Phenol Oxidase enzymes were found to increase up to 20ppm as compared to control and above this, all three enzymes showed decrease in activity. Uptake of Cu2O NPs nanoparticle by tomato seedlings was confirmed by atomic absorption spectroscopy.


 Key words: Nano-Cuprous Oxide, Flacourtia montana, Tomato, antioxidant enzymes, lipid peroxidation

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1. Jatav GK and Nirmal DE, Application of Nanotechnology in Soil-Plant System, J Soil Sci., 2013; 8(1):176-184.
2. Feizi H, Moghaddam PR, Shahtahmassebi N, and Fotovat A, Impact of bulk and nanosized titanium dioxide (TiO2) on wheat seed germination and seedling growth, Biol Trace Elem Res., 2012; 146:101-106.
3. Yin L, Cheng Y, Espinasse B, Colman BP, Auffan M, Wiesner M, Rose ZJ, Liu J, and Bernhardt ES, More than the ions: the effects of silver nanoparticles on Lolium multiflorum, Environ Sci Technol., 2011; 45:2360-67.
4. Awasthi KK, Awasthi Anjali K, Bhoot N, John PJ, Sharma SK, Awasthi K, Antimicrobial properties of electro-chemically stabilized organo-metallic thin films, Adv. Electrochem., 2013a; 1:1-6.
5. Awasthi KK, Awasthi A, Kumar N, Roy P, Awasthi K, and John PJ, Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells, Nanoparticle Res., 2012b; 15:1898.
6. Awasthi KK, Awasthi A, Verma R, Kumar N, Roy P, Awasthi K, and John PJ, Cytotoxicity, genotoxicity and alteration of cellular antioxidant enzymes in silver nanoparticles exposed CHO cells, RSC Adv., 2015a; 5:34927-35.
7. Awasthi KK, Verma R, Awasthi A, and Awasthi K, In vivo genotoxic assessment of silver nanoparticles in liver cells of Swiss albino mice using comet assay, Adv Mater Lett, 2015b; 6(3):187-193.
8. Barrena R., Casals E, Colon J, Font X and Sanchez A, Evaluation of the ecotoxicity of model nanoparticles, Chemosphere, 2009; 75:850-857.
9. Rezaei F, Moaveni P, and Mozafari H, Effect of different concentrations and time of nano TiO2 spraying on quantitative and qualitative yield of soybean (Glycine max L.) at Shahr-e-Qods. Iran. Biological Forum, 2015; 7:957–964.
10. Prapatsorn B, Kositsup B, Kumar P, Baruah S, and Dutta J, Toxicity of ZnO and TiO2 Nanoparticles on Germinating Rice Seed Oryza sativa L, Int. J. Biosci. Biochem. Bioinform, 2011; 1(4):282-285.
11. Lee CW, Mahendra S, Zodrow K, Li D, Tsai YC, and Braam J, Developmental phytotoxicity of metal oxide nanoparticles to Arabidopsis thaliana, Environ. Toxicol., 2010; 29:669-75.
12. Feizi H, Kamali M, Jafari L, and Moghaddam PR, Phytotoxicity and stimulatory impacts of nanosized and bulk titanium dioxide on fennel (Foeniculum vulgare Mill), Chemosphere, 2013; 91:506-511.
13. De la Rosa G, López-Moreno ML, De Haro D, Botez CE, Peralta-Videa JR and Gardea-Torresdey JL, Effects of ZnO nanoparticles in alfalfa, tomato, and cucumber at the germination stage: Root development and X-ray absorption spectroscopy studies, Pure Appl Chem., 2013; 85(12):2161-2174.
14. Lahiani MH, Dervishi E, Chen J, Nima Z, Gaume A, Biris AS and Khodakovskaya MV, Impact of Carbon Nanotube Exposure to Seeds of Valuable Crops, ACS Applied Materials & Interfaces, 2013; 5:7965-7973.
15. Shaw AK and Hossain Z, Impact of nano-CuO stress on rice (Oryza sativa L.) seedlings, Chemosphere, 2013; 93:906-915.
16. Parveen A, and Rao S, Effect of Nanosilver on Seed Germination and Seedling Growth in Pennisetum glaucum., J Clust Sci., 2015; 26:693-701.
17. Hu X, Mu L, Kang J, Lu K, Zhou R, and Zhou Q. Humic Acid Acts as a Natural Antidote of Graphene by Regulating Nanomaterial Translocation and Metabolic Fluxes, Environ Sci Technol., 2014; 48:6919-6927.
18. Wang Z, Xie X, Zhao J, Liu X, Feng W, White JC and Xing B, Xylem- and Phloem-Based Transport of CuO Nanoparticles in Maize, Environ Sci Technol., 2012; 46: 4434-4441.
19. Shobha G, Shashidhara KS, Vishwaprakash Mahadimane, and Ananda S, Plant Pathovars Inhibition from Copper Based Nanoparticles Synthesized from Leaf Extract of Flacourtia montana. J. Bionanosci., 2017; 11(6): 514–521.
20. Tapan A, Kundu S, Biswas AK, Tarafdar JC and Rao AS, Effect of Copper Oxide Nanoparticles on Seed Germination of Selected Crops, Journal of Agricultural Science and Technology, 2012; 2(1):815-823.
21. Savithramma N, Ankanna S, and Bhumi G, Effect of nanoparticles on seed germination and seedling growth of Boswellia ovalifoliolata – an endemic and endangered medicinal tree taxon, Nano Vis., 2012; 2:61–68.
22. Abdul-Baki AA, and Anderson JD, Vigor determination in soybean and seed multiple criteria, Crop Science, 1973; 13(6):630–633.
23. Wang X, Yang X, Chen S, Li Q, Wang W, Hou C, Gao X, Li W, and Wang S, Zinc Oxide Nanoparticles Affect Biomass Accumulation and Photosynthesis in Arabidopsis, Front Plant Sci., 2015; 6:1-9.
24. Lichtenthaler HK, Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. Method Enzymol., 1987; 148:350–382.
25. Misra HP and Fridovich I, Superoxide dismutase: “positive” spectrophotometric assays, Anal Biochem., 1977; 70:553-560.
26. Mohandas J, Marshal JJ, Duggin GG, Horvath JS and Tiller DG, Low activities of glutathione-related enzymes as factors in the genesis of urinary bladder cancer, Cancer Research, 1984; 44(11): 5086–5091.
27. Aebi H, Catalase in vitro, Methods of Enzymology, 1984; 105: 121-126.
28. Godson A, Lam M, Scaman CH, Clemens S, and Kermode A, Screening of phenylalanine ammonia lyase in plant tissues, and retention of activity during dehydration, J Sci Food Agric., 2008; 88(4):619–625.
29. Mahadevan A, and Sridhar R, Methods in Physiological Plant Pathology, 2nd ed. Sivakami Publications. 1982; 316.
30. Madhava Rao KV, and Sresty TVS, Antioxidative parameters in the seedlings of pigeonpea (CajanusCajan L. Millspaugh) in response to Zn and Ni stresses, Plant Sci, 2000; 157: 113-28.
31. Lee SK, Mbwambo ZH, Chung H, Luyengi L, Gamez EJ, Mehta RG, Kinghorn AD and Pezzuto JM, Evaluation of antioxidant potential of natural products, Comb Chem High Throughput Screen, 1988; 1(1):35–46.
32. Prieto P, Pineda M, and Aguilar M, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal Biochem., 1999; 269(2):337–341.
33. Lowry OH, Rosebrough NJ, Farr AL, and Randall RJ, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 1951; 193(1): 265–275.
34. Singh A, Singh NB, Hussain I, and Singh S, Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. Botrytis, J Biotech., 2017; 262: 11-27.
35. Song G, Hou W, Gao Y, Wang Y, Lin L, Zhang Z, Niu Q, Ma R, Mu L, and Wang H, Effects of CuO nanoparticles on Lemna Minor, Bot Stud., 2016; 57(3):1-8.
36. Feizi H, Amirmoradi SH, Abdollahi F, and Jahedi Pour S, Comparative effects of nanosized and bulk titanium dioxide concentrations on medicinal plant Salvia officinalisL, Annual Review & Research in Biology, 2013; 3(4):814-829.
37. Clement L, Hurel C, and Marmier N, Toxicity of TiO2 nanoparticles to cladocerans, algae, rotifers and plants-Effects of size and crystalline structure, Chemosphere, 2013; 90(3):1083-1090.
38. Fatma AF and Nivien AN, Green Synthesis of Silver Nanoparticles Using Leaf Extract of Rosmarinus officinalis and Its Effect on Tomato and Wheat Plants, J of Agri Sci., 2015; 7(11): 277-287.
39. Morteza E, Moaveni P, AiabadiFarahani H, and Kiyani M, Study of photosynthetic pigments changes of maize (Zea mays L.) under nano TiO2 spraying at various growth stages, Springer Plus, 2013; 2:1-5.
40. Kareem AM, El-Naggar M, Ramamoorthy K, Alawadhi H, Elnaggar A, Wartanian S, Ibrahim E, and Hani H, Copper Nanoparticles Induced Genotoxicty, Oxidative Stress, and Changes in Superoxide Dismutase (SOD) Gene Expression in Cucumber (Cucumis sativus) Plants, Frontiners in plant science, 2018; 9 (872):1-13.
41. Hesham FA, Ehab MR, Metwali MP, Amal F, and Aldhebiani Y, Impact of Zinc Oxide Nanoparticle on Callus Induction, Plant Regeneration, Element Content and Antioxidant Enzyme Activity In Tomato (Solanum Lycopersicum Mill.) Under Salt Stress, Archives of Biological Sciences, 2016; 68(4): 723-35.
42. Rao S, and Shekhawat GS, Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea. Biotech., 2016; 6:2-12.
43. Zaka M, Abbasi BH, Rahman L, Shah A, and Zia M, Synthesis and characterisation of metal nanoparticles and their effects on seed germination and seedling growth in commercially important Eruca sativa. IET Nanobiotechnology, 2016; 10 (3):1-8.
44. Uhram S, Heeju J. Bruce W, Jinkyu R, Younghun K, Jongheop Y, and Eun J, Functional analyses of nanoparticle toxicity: A comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum), Ecotoxicology and Environmental Safety, 2013; 93: 60-67.
45. Shankramma K, Yallappa S, Shivanna MB, and Manjanna J, Fe2O3 magnetic nanoparticles to enhance S. lycopersicum (tomato) plant growth and their biomineralization, Applied Nanoscience. 2016; 6(7):983-990.
46. Costa MVJ, and Sharma PK, Effect of copper oxide nanoparticles on growth, morphology, photosynthesis, and antioxidant response in Oryza sativa, Photosynthetica, 2016; 54 (1):110-119.
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How to Cite
S, A., G, S., KS, S., & Mahadimane, V. (2019). Nano-cuprous oxide enhances seed germination and seedling growth in Lycopersicum esculentum plants. Journal of Drug Delivery and Therapeutics, 9(2), 296-302. https://doi.org/10.22270/jddt.v9i2.2554