Formulation development, characterization and assessment of In-Vitro antifungal efficacy against Candida albicans of diallyl disulphide liposomal gel using 32 factorial design
Objective: The aim of this study was to develop the diallyl disulphide (DADS) liposomal dispersion and further the dispersion was to formulate into gel.
Methods: The DADS liposomal system was developed using thin film hydration method. 32 factorial design was employed to study the effect of various factors to obtain the optimized liposomal system. Further, it was converted to gel using suitable gelling agent. The optimized liposomal system and gel formulations were evaluated for various parameters. The optimized gel was evaluated for its in-vitro antifungal efficacy. The stability studies for gel were carried as per International conference on harmonisation (ICH) guidelines.
Results: The optimized liposomal system was having vesicle size of 208.6nm, % entrapment was 91.7±1.16% and loading was 11.12±0.23%. % drug permeation was 61.10±0.85%. The zeta potential showed moderate stability. The optimized DADS liposomal gel showed satisfactory results. Ex-vivo permeation studies showed that DADS liposomal gel possesses sustained release and drug retention study proved better retention of DADS from liposomal gel than conventional gel in skin. The stability studies showed DADS liposomal gel was stable for 30 days at 25℃/60%RH, 40℃/75%RH and refrigeration (5±3℃).
Conclusion: The results from the present study show that the sustained release profile of optimized DADS liposomal gel. The retention of DADS from liposomal gel was more than from conventional gel which helps in better pharmacological activity with least side effects.
Keywords: Diallyl disulphide (DADS), liposomal gel, permeation, sustained release and antifungal activity.
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