Formulation and In-vitro evaluation of liposomal drug delivery system of metformin HCl
Metformin is widely used for the treatment of diabetes; the intention of the present study was to formulate Metformin HCl liposomes for a sustained drug delivery system. It have the advantages of dose reduction, less dosing frequency, minimize the side effect, prolong the action of drug and thus achieve better patient compliance. The liposomes were prepared by physical dispersion and ether injection method. Soya lecithin and cholesterol were used for encapsulating the drug, it facilitates to release the medicaments in sustained manner. Chloroform, ether and methanol were used as a solvent. Phosphate buffer pH 6.8 was used as a hydration medium for loading the drug. The final liposome was evaluated in various quality parameters of drug entrapment efficiency, morphological analysis, particle size analysis, in-vitro drug release studies and stability studies. In the two methods of metformin liposome formulation the ether injection method showed prolonged action when compared to physical dispersion method. In the parameters of drug entrapment and stability physical dispersion method was shows better results.
Keywords: Physical dispersion, ether injection, soya lecithin, cholesterol, morphological analysis, metformin.
2. Kalra N, Jeyabalan G. Niosomes: a versatile drug delivery system. Research journal of life sciences, bio-informatics, pharmaceutical and chemical science, 2016; 2(4):44-54.
3. Kotturi N. Novel drug delivery system. Research & Reviews: Journal of Pharmaceutics and Nanotechnology, 2015; 3(2): 32-36.
4. Kant shashi, Kumar satinder and Prashar bharath. A complete review on: Liposomes. International research journal of pharmacy, 2012; 3(7):10-16.
5. Akbarzadeh A, Rezaei-Sadabady R, Davaran S, Joo SW, Zarghami N, Hanifehpour Y, Samiei M, Kouhi M, Nejati-Koshki K. Liposome: classification, preparation, and applications. Nanoscale Research Letters, 2013; 8(102):1-9.
6. Suggy Chrai S, Murari R, Ahmad I. Liposomes: A Review. Biotech Trends, 2001; 14(11):10–14.
7. Andreas W, Karola-Uhl. Liposome technology for industrial purposes. Journal of Drug Delivery; 2011:1-9.
8. The United States Pharmacopoeia. The National Formulary, USP 94 NF19, Asian Ed; 2000: 2232.
9. Dhabale PN, Seervi C. Simultaneous UV spectrophotometric method for estimation of Metormin Hydrochloride in Tablet Dosage orm. Inter. J. Chem. Tech Res; 2010: 813-817.
10. Parashar V, Ahmad D, Gupta SP, Upmanyu N, Parashar N. Formulation and evaluation of biodegradable microspheres of Tinidazole. International Journal of Drug Delivery; 2010; 2:238-241.
11. Mutalik S, Naha A, Usha AN, Anju P, Ranjith AK, Musmade P, K. Manoj and Prasanna. Preparation, in vitro, preclinical and clinical evaluation of once daily sustained release of tablets of aceclofenac. Arch Pharm Res; 2007; 30:222-232.
12. Shivhare UD, Ambulkar DU, VMathur VB, Bhusari KP, Godbole MD, Formulation and evaluation of pentoxifylline liposome formulation. Digest journal of nanomaterials and biostructures, 2009; 4(4):857-862.
13. Laouini, C. Jaafar-Maalej, I. Limayem-Blouza, S. Sfar, C. Charcosset1, and H. Fessi. Preparation, characterization and applications of liposomes: State of the Art. Journal of Colloid Science and Biotechnology, 2012; 1:147–168.
14. Senthilkumar KL, Ezhilmuthu RP, Praveen P. Preparation and characterization of nabumetone liposomes. International journal of life science biotechnology and pharma research, 2012; 1(1):81-86.
15. Lankalapalli S, Vinai Kumar Tenneti VS, Adama R, Preparation and evaluation of liposome formulations for poorly soluble drug itraconazole by complexation, Scholars Research Library, 2015; 7(8):1-17.
16. The United State of Pharmacopoeia 24/NF26. Asian Ed. The official compendia of United States of Pharmacopoeial convection Inc. Rockville; 1995:1015-1016.
18. Pai RS, Devi KV. Lamivudine Liposomes for Transdermal Delivery-Formulation, Characterization, Stability, and Invitro Evaluation, International Journal of. Pharmaceutical Science and Nanotechnology, 2009; 1: 317-326.
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