Effect of Ethanolic Extract of Bauhinia variegata and Commiphora mukul in Regulating Thyroid Stimulating Hormone in Hypothyroidism Induced Albino Wistar Rats
The present clinical interventions used for the management of the hypothyroidism are still associated with various side effects. In order to avoid this, the aim of the present investigation is to evaluate the effect of Bauhinia variegata and Commiphora mukul in regulating serum thyroid stimulating hormone levels in rats. Ethanolic extract of Bauhinia variegata (non-woody aerial parts) and Commiphora mukul(gum resin) was prepared via hot solvent extraction in a Soxhlet apparatus. Preliminary phytochemical screening showed that the ethanolic extract of Bauhinia variegates linn has all the phytoconstituents except phytosterol, fixed oil and fats. The Commiphora mukul gum resin extract showed the presence of all phytoconstituents except alkaloids, fixed oil and mucilage.The oral toxicity studies showed no abnormalities at the maximum tolerated dose level of 2000 mg/kg body weight. Methimazole were used to induce hypothyroidism in the rats. The animals were treated with different individual and combined dosages (100, 200 and 400 of two plant extracts. Serum TSH level analysis showed that a combination of high dose (400mg /kg body weight) of Bauhinia variegate and Commiphora mukul gum resin extracts showed a significant effect in lowering serum TSH levels raised due to hypothyroidism. To conclude, a combined extract of plants could be a potential therapeutic alternative for the management of hypothyroidism.
Keywords: Bauhinia variegate Linn., Commiphora mukul, Hypothyroidism, Thyroid stimulating hormones, Methimazole.
2. Nair RR, Joseph L, Rahees T. Role of ethanolic extract of bauhinia purpurea leaves on amelioration ofhyperthyroidism in LT4 induced female albino wistarrats. Journal of Pharmacology and Toxicology Studies: Research and Reviews. 2015; 3(4):1-10.
3. Shi YB, Ritchie JW, Taylor PM. Complex regulation ofthyroid hormone action: multiple opportunities forpharmacological intervention. Pharmacology and Therapeutics. 2002; 94(3):235-51.
4. Bagcchi S. Hypothyroidism in India: more to be done. The Lancet. 2014; 2:778.
5. Nagarathna PKM, Deepa KJ. Study on ant thyroidproperty of some herbal plants review article. International Journal of Pharmaceutical Science: Review and Research. 2013; 23(2):203-11.
6. Wangchuk P. Therapeutic applications of natural products in herbal medicines, biodiscovery programs, and biomedicine. Journal of Biologically Active Products from Nature. 2017; 8(1):1-20.
7. Gupta A, Wamankar S, Gidwani B, Kaur CD. Herbaldrugs for thyroid treatment. International Journal of Pharmacy Biological Science. 2016;6(1):62-70.
8. Bharthi V, Kavya N, Shubhashree MN, Bhat S. Herbalapproach to management of thyroid disease - a review. Journal of Ayurveda Herbal Medicine. 2017; 3(1):48-52.
9. Al-Snafi AE. The Pharmacological Importance ofBauhinia variegata. A review. International Journal ofPharmaceutical Science and Research.2013; 4(12):160-164.
10. Singh KL, Singh DK, Singh VK. Multidimensional uses of medicinal plant kachnar (Bauhinia variegata Linn.).American Journal of Phytomedicine and Clinical Therapeutic. 2016; 4(2):58-72.
11. Deng R. Therapeutic effects of guggul and its constituent guggul sterone: cardiovascular benefits. Cardiovascular Drug Reveiw.2007; 25(4):375-390.
12. Ragavi R, Saritha Surendran A. Commiphora mukul: AnOverview. Research Journal of Pharmaceutical Technology. 2018; 11(7):3205-3208.
13. Singha MB, Sarma T, Lahkar M. Anti-hypothyroid potential of Bauhinia variegateand Commiphora mukul extracts in albino wistarrats: A histopathological investigation. Journal of Pharmacognosy and Phytochemistry 2019; 8(1):1736-1740.
14. Yadav M, Chatterji S, Gupta SK, Watal G. Preliminary phytochemical screening of six medicinal plants used intraditional medicine. International Journal of Pharmacy and Pharmaceutical Science. 2014; 6(5):539-542.
15. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H.Phytochemical screening and extraction: A Review. International Journal of Pharmaceutical Science. 2011; 1(1):98-106.
16. OECD. OECD guideline for testing of chemicals. Testnumber 423: Acute oral toxicity – Acute toxic classmethod. OECD Publishing, 2018.http://www.oecd.org/env/ehs/testing/oecdguidelinesforthetestingofchemicals.htm. 20 Feb.
17. Azharuddin M, Atif M, Ahmed MI, Bakhtiary SA, Ibrahim M. Evaluation of anti-thyroid activity of Ficus racemosalinn bark in male rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2015; 7(3): 118-122.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).