Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).
Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds.
Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis.
Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial and antidiabetic compounds.
Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various medicinal properties to cure dangerous diseases and disorders.
Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.
2. Fabricant D, Farnsworth N. The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives. 2001; 109(1):69-75.
3. Wamidh HT. Anticancer and Antimicrobial Potential of Plant-Derived Natural Products. In: Iraj Rasooli, ed. Phytochemicals–Bioactivities and Impact on Health. Croatia. 2011; 141-58.
4. Alviano D, Alviano A. Plant extracts: search for a new alternative to treat microbial diseases. Current Pharmaceutical Biotechnology. 2009; 10(1):106-21.
5. Nostro A, Germano MP, Danelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity; Lett. Appl. Microbial 2000; 30: 379-384.
6. Azaizeh H, Fulder S, Khalil K, Said O. Ethnobotanical knowledge of local Arab practitioners in the Middle Eastern region. Fitoterapia. 2003; 74(1-2):98- 108.
7. Tabuti JRS, Lye KA, Dhillion SS. Traditional herbal drugs of Bulamogi, Uganda: plants, use and administration. Journal of Ethnopharmacology. 2003; 88(1):19-44.
8. Misra A. J Med Plants Res. 2009; 3:1140-1146.
9. de-Fatima A, Modolo LV, Conegero LS, Pilli RA, Ferreira CV, Kohn LK, de- Carvalho JE, Lactones and their derivatives: biological activities, mechanisms of action and potential leads for drug design, Curr Med Chem. 2006; 13:3371-3384.
10. Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement. Altern. Med. 2006; 6:35-41.
11. Ghazi A. Introduction to pharmacognosy, Ahmadu Bello University Press, Ltd. Zaria, Nigeria. 1990; 45-47.
12. Mandal V, Mohan Y, Hemalatha S. Pharmacog Rev. 2007; 1:7-18.
13. Dobelis IN. Magic and Medicine of plants. The Reader Digest Association Inc., New York, Montreal. 1993; 8-48.
14. Nascimento GGF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazil Journal Microbiology. 2000; 31(4): 886-891.
15. Robertson DG. Metabonomics in toxicology: A review. Toxicol. Sci. 2005; 85:809-22.
16. Fernie AR, Trethewey RN, Krotzky AJ, Willmitzer L. Metabolite profiling: From diagnostics to systems biology. Nat Rev Mol Cell Biol. 2004; 5:763-9.
17. Kell DB, Brown M, Davey HM, Dunn WB, Spasic I, Oliver SG. Metabolic footprinting and systems biology: The medium is the message. Nat Rev Microbiol. 2005; 3:557-65.
18. Subramanian S, Ramakrishnan N. Chromatographic fingerprint analysis of Naringi crenulata by HPTLC technique. Asian Pacific Journal of Tropical Biomedicine. 2011; 1(2):195-8.
19. Mariswamy Y, Gnaraj WE, Johnson M. Chromatographic fingerprint analysis of steroids in Aerva lanata L by HPTLC technique. Asian Pacific journal of tropical biomedicine. 2011; 1(6):428-33.
20. Cowan MM. Plants products antimicrobial agents. Clin Microbial Rev. 1999; 14:564‐584.
21. Clare BA, Conroy RS, Spelman K. The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day, J Altern Complement Med. 2009; 15 (8):929-34.
22. Sigstedt SC, Hooten CJ, Callewaert MC, Jenkins AR. Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells, Int J. Oncol. 2008; 32(5):1085-90.
23. Clare BA, Conroy RS, Spelman K, The diuretic effect in human subjects of an extract of Taraxacum officinale folium over a single day, J Altern Complement Med. 2009; 15(8):929-34.
24. Sigstedt SC, Hooten CJ, Callewaert MC, Jenkins AR et al., Evaluation of aqueous extracts of Taraxacum officinale on growth and invasion of breast and prostate cancer cells, Int J Oncol. 2008; 32(5):1085-90.
25. Harborne JB. Phytochemical Methods. London: Chapman and Hall Ltd.; 1973; 49-188.
26. Remmal A, Bouchikhi T, Rhayour K, Ettayebi M. Improved method for the determination of antimicrobial activity of essential oils in agar medium, J. Essent. Oil Res. 1993; 5:179-184.
27. Mariat George. Phytochemical and Antioxidant studies on the essential oil of the rhizome of Curcuma aeruginosa (Roxb). International Research Journal of Pharmacy. 2015; 6(8): 573-579.
28. Ware GW: The Pesticides Book,. Thomson Publications, Fresno, CA, USA. 1994.
29. Voda K, Boh B, Vrtacnik M, Pohleven F. Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the white-rot Trametes versicolor and the brown-rot Coniophora puteana. Int. Biodeterior. Biodegrad. 2003; 51:51-59.
30. Jessica A, Rao MRK, Anthony J, Prabhu K, Johnson WMS, Balasubramanian BS, Sundaram L, Dinakar S. The GC-MS Study of One Ayurvedic Preparation Katakakhadiradi Kashayam. Int. J. Pharm. Sci. Rev. Res. 2016; 39(2):216-224.
31. Xu F, Yuan QP, Zhu Y. Improved production of lycopene and β-carotene by Blakeslea trispora with oxygen-vectors. Process Biochem. 2007; 42:289–293.
32. Lai LT, Tsai TH, Wang TC. Application of oxygen vectors to Aspergillus terreus cultivation. J. Biosci. Bioeng. 2002; 94:453–459.
33. Peng C, Huang H, Ji X, Liu X, Ren L, Yu W, You J, Lu J. Effects of n-hexadecane concentration and a two-stage oxygen supply control strategy on arachidonic acid production by Mortierella appina ME-1. Chem. Eng. Technol. 2010; 33:692–697.
34. Ramalingam Vidhya and Rajangam Udayakumar. Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Ethanolic Extracts of Aerva lanata (L.). International Journal of Biochemistry Research & Review 2015; 7(4):192-203.
35. Cerchiara T, Blaiotta G, Straface VS, Belsito E, Liguori A, Luppi B, Bigucci F, Chidichimo G. Biological Activity of Spartium junceum L. (Fabaceae) Aromatic Water. Nat. Resources, 2013; 4:229-234.
36. Da Silva CEL, Minguzzi S, da Silva RCL, Matos MFC, Toffoli D, de Carvalho JE, Ruiz ALTG, da Costa WF, Simionatto E. Chemical Composition and Cytotoxic Activity of the Root Essential Oil from Jatropha ribifolia (Pohl) Bill (Euphorbiaceae). J Braz. Chem. Soc., 2015; 26(2):233-238.
37. Gnanasundaram I, Balakrishnan K. Characterization of Bioactive Compounds in Ethanolic Extract of Cissus vitiginea Leaves Using GC-MS Technique. IOSR Journal of Applied Chemistry. 2017; 10, 9(3):24-27.
38. Viuda-Martos M, Navajas Y, Zapata, ESJ, López F, Pérez-Álvarez JA. Flavour Frag J 2010; 25:13-19.
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