Anticancer (liver cancer cell lines) and antioxidant activity of Cassia auriculata flower extract from acetone and methanol solvents

  • K Padmalochana Head of the Department of Biochemistry, Sri Akilandeswari Women’s College, Wandiwash – 604408, TN, India

Abstract

This research paper was communicated to elucidate the antioxidant and anticancer activities of acetone and methanol extract of Cassia auriculata flower. Antioxidant activity was assessed against DPPH and hydroxyl free radical. The anticancer activity was examined by inhibition on liver cancer cell lines. The highest antioxidant activity was found for methanol extract (IC50: 46.28 μg/ml) than acetone extract (IC50: 52.60 μg/ml) on DPPH free radical. The IC50 values for methanol and acetone extract was found to be 47.05 and 56.01±1.85 µg/ml, respectively on hydroxyl radical. The highest anticancer activity was found for methanol extract to be 36.10±2.46 µg/ml. Among these extract methanol extract have high antioxidant and anticancer activity.


Keywords: Antioxidant, anticancer, Plant

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Author Biography

K Padmalochana, Head of the Department of Biochemistry, Sri Akilandeswari Women’s College, Wandiwash – 604408, TN, India

Head of the Department of Biochemistry, Sri Akilandeswari Women’s College, Wandiwash – 604408, TN, India

References

1. Parmar Y and Chakraborthy GS. Evaluation of Cassia auriculata leaves for its potent biological activity J Pharmacol 2011; 2:128-33.
2. Bhagwati U. Utilization of medicinal plants by the rural women of Kulu, Himachal Pradesh. Indian J Trad Knowl 2003; 2:366-270.
3. Anushia C, Sampathkumar P and Ramkumar L, Antibacterial and Antioxidant Activities in Cassia auriculata, Global Journal of Pharmacology, 2009; 3(3):127-130
4. Rajagopal S, Manickam P, Periyasamy V, Namasivayam N. Effect of Cassia auriculata leaf extract on lipids in rats with alcoholic liver injury. Asia Pac J Clin Nutr 2002; 11:157-163
5. Rajagopal J, Anderson WJ, Kume S, Martinez OI, Melton DA. Insulin staining of ES cell progeny from insulin uptake. J Science 2003; 299:363.
6. Rastogi RP and Mehrotra BN .Compendium of Indian Medicinal plants. Central Drug Research Institute Lucknow and National Institute of Science Communication, 1994; 1-5
7. Senthilkumar PK, Reetha D. Isolation and identification of antibacterial compound from the leaves of Cassia auriculata. Europ Rev Med Pharmacol Sci 2011; 15:1034-1038.
8. Vedavathy S and Rao KN. Antipyretic activity of six indigenous medicinal plants of Tirumala Hills J Ethnopharmacol. 1991; 33:193-6.
9. Kumar RS et al. Effect of Cassia auriculata leaf extract on lipids in rats with alcoholic liver injury. Asia Pac J ClinNutr 2002; 11(2):157–63.
10. Senthilkumar P.K, Gajendiran K, Analysis The Nephro Protective Activity of The Oleanolic Acid Isolated from the Medicinal Plant - Cassia Auriculata (Linn.), Indian Journal of Applied Research, 2014; 4(7):441-442
11. Kumar P, Rao D, Lakshmayya G, Ramachandra Setty S. Nephroprotective and Nitric oxide scavenging activity of tubers of Momordica tuberose in rats. Avicenna J Med Biotech 2011; 3:87–93.
12. Latha M and Pari L. Antihyperglycaemic effect of Cassia auriculata in experimental diabetes and its effects on key metabolic enzymes involved in carbohydrate metabolism. ClinExpPharmacol Physiol 2003; 30:38–43.
13. Maneemegalai S and Naveen N. Evaluation of Antibacterial Activity of Flower Extracts of Cassia auriculata Ethnobotanical Leaflets 2010; 14:8-20.
14. Mario DA, et al. Cardiovas Res, 2004; 63:593-602.
15. Varghese GK et al. Antidiabetic components of Cassia alata leaves: identification through α-glucosidase inhibition studies. Pharm Biol 2013; 51(3):345-9.
16. Meenupriya J., Sahaya Vinisha A. and Priya P. Cassia alata and Cassia auriculata – Review of their bioactive potential, World J Pharm Sci 2014; 2(12):1760-1769
17. Ramesh K., Manohar S and S. Rajeshkumar, Nephroprotective activity of Ethanolic Extract of Orthosiphon stamineus Leaves on Ethylene Glycol induced Urolithiasis in Albino Rats, International Journal of PharmTech Research, 2014; 6(1):403-408
18. Sivakumar V, Rajeshkumar S. Protective effect of Andrographis paniculata on hyperglycemic mediated oxidative damage in renal tissues of diabetic rats. The Journal of Phytopharmacology 2015; 4(6):287-294.
19. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994; 344:721-724.
20. Harborne JB, Phytochemical method. A Guide to Modern techniques to Analysis. (2nd Edn), Chapman and Hall, New York, 1973; 10-75.
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Padmalochana K. Anticancer (liver cancer cell lines) and antioxidant activity of Cassia auriculata flower extract from acetone and methanol solvents. JDDT [Internet]. 15Dec.2018 [cited 2Mar.2021];8(6-s):274-8. Available from: http://jddtonline.info/index.php/jddt/article/view/2130