IN-VITRO FREE RADICAL SCAVENGING POTENTIAL OF FLAVONOIDS WITH SYNERGISTIC EFFECT OF THEIR COMBINATION
The present study was carried out to evaluate the possible synergistic interactions on antioxidant efficacy of some selected flavonoids in the present study, synergistic antioxidant effects of two flavonoids, rutin and quercetin, have been investigated by. DPPH, NO, free radical scavenging assays. Biological free radicals are highly unstable molecules that react with various organic substrates such as lipids, proteins, DNA causing cellular injury. At high concentrations, they generate oxidative stress, a damaging process that can damage all cell structures. At present, the research is focused on the use of antioxidants in preventing many diseases caused by the free radicals. The study was designed to evaluate in vitro antioxidant potential of quercetin, rutin, individually and synergistic antioxidant effects by using various in-vitro antioxidant assays i.e. DPPH, NO, assays. The scavenging effect of quercetin, rutin and standard on the DPPH radical was 83±2.71 %, 79±1.51% and 86±1.04 % at 60μg/ml. On NO Quercetin, rutin and standard exhibited 83±0.09 %, 76±0.09 % and 85±0.22%. The scavenging activity increased in a dose dependent manner. The results indicate drugs shows significant free radical scavenging and their combination demonstrated considerable synergistic effect as compared to standard.
Keywords: Quercetin, Rutin, Free radical scavenging, Antioxidant
2. Farombi, E.O. African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. Afr. J. Biotech. 2003; 662-671.
3. Singh A, Malhotra S, Subban R. Anti-inflammatory and analgesic agents from Indian medicinal plants. Int. J. Integr. Biol. 2008; 3:57.
4. Halliwell B, Gutteridge J.M.C. Free radicals in biology and medicine. Clarendon Press: Oxford, UK, 2007
5. Willcox J.K, Ash S.L., Catignani G.L. Antioxidants and prevention of chronic disease. Crit. Rev. Food Sci. Nutr. 2004; 44:275-95.
6. Genestra M. Oxyl radicals, redox-sensitive signaling cascades and antioxidants. Cellsig. 2007; 19:1807-1819.
7. Halliwell B. Biochemistry of oxidative stress. Biochem. Soc. Trans. 2007; 35:1147-1150.
8. Young I, Woodside J. Antioxidants in health and disease. J. Clin. Pathol. 2001; 54:176–186.
9. Iris F.F. Evolution of dietary antioxidants. Comparative Biochemistry and Physiology 2003; 136:113-126.
10. Wolfe K, Xianzhong W.U., Liu R.H. Antioxidant activity of apple peels. J. Agricul. Food Chem. 2003; 51:609–614.
11. Westhuizen JHV, Sisa MB, Ferreira SLD. Photochemistry of Flavonoids. Molecules. 2010; 15:5196-5245.
12. Bouktaib Metal. Regio- and stereoselective synthesis of the major metabolite of quercetin, quercetin-3-O-b-D-glucuronide. Tetrahedron Letters, 2002; 43:6263-6266.
13. Kleemann R, Verschuren L, Morrison M, Zadelaar S, Van Erk MJ, Wielinga PY and Kooistra, T. Anti-inflammatory, anti-proliferative and anti-atherosclerotic effects of quercetin in human in vitro and in vivo models. Atherosclerosis. 2011; 218:44-52.
14. Shafi N, Ikram M. Quantitative survey of rutin containing plants. Part 1. Pharmaceutical Biol. 1982; 20:183-186.
15. Jasuja R, Passam FH, Kennedy DR, Kim SH, Hessem LV, Lin L, Bowley SR, Joshi SS, Dilks JR, Furie B, Furie BC. and Flaumenhaft R. Protein disulfide isomerase inhibi-tors constitute a new class of antithrombotic agents. Journal of Clinical Investigation. 2012; 122(6):2104–2113.
16. Sofic E, Copra-Janicijevic A, Salihovic M, Tahirovic I and Kroyer G. Screening of medicinal plants extracts for quercetin-3-rutinoside (rutin) in Bosnia and Herzegovina. Medicinal plants. 2010; 2(2):97-102.
17. Fathiazad F, Delazar A, Amiri R and Sarker SD. Extraction of flavonoids and quantification of rutin from waste tobacco leaves. IJPR. 2006; 5:222-227.
18. Dnyaneshwar M, Nagmoti et al.: Antioxidant activity and free radical-scavenging potential of Pithecellobium dulce Free Radicals and Antioxidants 2012; 2(2):39-40
19. Ganapaty S, Chandrashekhar VM, Chitme HR, Lakashami NM. Free radical scavenging activity of gossypin and nevadensin: An in vitroevaluation. Indian J Pharmacol. 2007; 39(6):281–3.
20. Prasad KN, Divakar S, Shivamurthy GR, Aradhya SM, “Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk),” Journal of the Science of Food and Agriculture, 2005; 85(9):1461–1468.
21. Shirwaikar Prabhu KS, I. Punitha ISR, “In vitro antioxidant studies of Sphaeranthus indicus (Linn),” Indian Journal of Experimental Biology, 2006; 44(12):993–99.
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