GC-MS ANALYSIS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ETHANOL EXTRACT OF LEAVES OF AEGLE MARMELOS (L.) CORRÊA

  • Arumugam Perumal Armats Biotek Training and Research Institute, Maduvinkarai, Guindy, Chennai – 600 032, India
  • Saraswathi Krishna Karpaga Vinayaga College of Engineering and Technology, Madhuranthagam, Kancheepuram – 603 308, India
  • Madhusree Madhusree Meenakshi College for Women, Kodambakkam, Chennai – 600 024, India

Abstract

The aim of the present study was to evaluate the antioxidant activities of leaves of Aegle marmelos and to identify the bioactive compounds by performing GC-MS analysis resulting in the presence of volatile and semi volatile compounds. The IC50 of DPPH˙ radical scavenging assay was 78.36µg/mL concentration respectively. Also, the IC50 of Phosphomolybdenum reduction and ferric reducing power assay were 41.35 and 20.58µg/mL concentration respectively. Also, total phenolic and flavonoid content were determined, in which flavonoids were found to be predominantly higher. The results of this study portray the effective antioxidant activity of Aegle marmelos and further studies are required to isolate the active compounds from various parts of this species and their mode of action. From the study it can be concluded that the plant might be promising as a curative for many diseases associated with free radicals.

Keywords: Free radicals, Aegle marmelos, Antioxidant, Dot plot, DPPHË™ assay, IC50, GCMS.

Downloads

Download data is not yet available.

Author Biographies

Arumugam Perumal, Armats Biotek Training and Research Institute, Maduvinkarai, Guindy, Chennai – 600 032, India
Armats Biotek Training and Research Institute, Maduvinkarai, Guindy, Chennai – 600 032, India
Saraswathi Krishna, Karpaga Vinayaga College of Engineering and Technology, Madhuranthagam, Kancheepuram – 603 308, India
Karpaga Vinayaga College of Engineering and Technology, Madhuranthagam, Kancheepuram – 603 308, India
Madhusree Madhusree, Meenakshi College for Women, Kodambakkam, Chennai – 600 024, India

Meenakshi College for Women, Kodambakkam, Chennai – 600 024, India

References

1. Rajan S, Gokila M, Jency P, Brindha P, Sujatha RK, Antioxidant and phytochemical properties of Aegle marmelos fruit pulp: International journal of current pharmaceutical research, 2011; 3(2). ISSN-0975-7066.
2. Ariharan VN and Nagendra Prasad P, Mahavilva - a sacred tree with immense medicinal secrets: a mini review: Rasayan J Chemistry, 2013; 6(4):342-352.
3. Orwa C, Mutua A, Kindt R, Jamnadass R and Simons A, An introduction to Aegle Marmelos Agro Data, 2009, 4(2), 1-5.
4. Lambole VB, Murti K, Kumar U, Sandipkumar PB and Gajera V, International Journal of Pharmaceutical Sciences Review and Research, 2010; 5:67-72.
5. Harborne JB, Phytochemical Methods, A guide to Modern Techniques of Plant analysis, second ed. Chapman and Hall, London. 1998, 54-84.
6. Soler-Rivas C, Espin JC, Wichers HJ, An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochem. Analysis, 2000; 11:1-9.
7. Khalaf NA, Shakya AK, Al-Othman A, El-Agbar Z, Farah H, Antioxidant activity of some common plants, Turk J. Biol, 2008; 32:51-55.
8. Prieto P, Pineda M, Aguilar M, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 1999; 269:337–41.
9. Hennebelle T, Sahpaz S, Gressier B, Joseph H and Bailleul F, Antioxidant and Neurosedative Properties of Polyphenols and Iridoids from Lippia alba. Phytotherapy Research, 22; 2008:256-258.
10. Makari HK, Haraprasad N, Patil HS, Ravikumar, In Vitro Antioxidant Activity of The Hexane And Methanolic Extracts Of Cordia wallichii and Celastrus paniculata, The Internet J.Aesthetic and Antiaging Medicine, 2008; 1:1-10.
11. Spanos GA, and Wrosltad RE, Influence of processing and storage on the phenolic composition of Thompson seedless grape juice, Journal of Agricultural & Food Chemistry, 1990; 38:1565-1571.
12. Liu X, Dong M, Chen X, Jiang M, Lv X and Yan G, Antioxidant activity and phenolics of endophytic Xylaria sp. from Ginkgo biloba, Food Chemistry, 2007; 105:548-554.
13. Stahl E, Thin Layer Chromatography, 2nd ed., Springer Pvt. Ltd., New Delhi, 2005, 85.
14. Eloff JN, Which extractant should be used for the screening and isolation of antimicrobial components from plants, J. Ethnopharmacol, 1998; 60:1-8.
15. Kayser O and Kolodziej H, Antibacterial activity of extracts and constituents of Pelargonium sidoides and Pelargonium reniforme, Planta Med. 1997; 63:508-510.
16. Egan D, O’Kennedy R, Moran E, Cox D, Prosser E and Thornes D, The pharmacology, metabolism, analysis, and applications of coumarin and coumarin-related compounds, Drug Metabolism Reviews, 1990; 22(5):503-529.
17. Sergeant, L. & May, E. Agonistsantagonists derived from desomorphine and metopon. Journal of Medicinal Chemistry, 1970; 13:1061.
18. Ryu KR, Choi JY, Chung S, Kim DH, Anti-scratching behavioral effect of the essential oil and phytol isolated from Artemisia princeps Pamp. in mice, Planta Med. 2011; 77(1):22-6.
19. McGinty D, Letizia CS, Api AM, Fragrance material review on phytol. Food Chem Toxicol. 2010; 48 Suppl (3):S59-63.
20. Lim SY, Meyer M, Kjonaas RA, Ghosh SK, Phytol-based novel adjuvants in vaccine formulation; assessment of safety and efficacy during stimulation of humoral and cell-mediated immune responses. J Immune Based Ther Vaccines, 2006; 30(4):6.
21. Inoue Y, Hada T, Shiraishi A, Hirose K, Hamashima H, Kobayashi S, Biphasic effects of geranylgeraniol, teprenone, and phytol on the growth of Staphylococcus aureus, Antimicrob Agents Chemother, 2005; 49(5):1770-4.
22. Justyna M, Kamil K, Anna K, Flavonoids as Important Molecules of Plant Interactions with the Environment, Molecules, 2014; 19:16240-16265.
Statistics
42 Views | 45 Downloads
How to Cite
Perumal, A., Krishna, S., & Madhusree, M. (2018). GC-MS ANALYSIS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ETHANOL EXTRACT OF LEAVES OF AEGLE MARMELOS (L.) CORRÊA. Journal of Drug Delivery and Therapeutics, 8(4), 247-255. https://doi.org/10.22270/jddt.v8i4.1781