Wound Repair and Regenerating Effect of Eugenol Isolated from Ethyl Acetate Soluble Fraction of Ethanolic Extract of Cinnamomum tamala Leaves in STZ Diabetic Rats
Diabetes is a chronic hyperglycaemic disorder; leads to developed several complications including delayed wound healing after any injury. These non-healing wound ends up to organ or limb salvage. The available modern medications are not capable to fully control over these complications. There are several evidences that these complications can easily treated by using herbal of folklore medicines. The leaves of Cinnamomum tamala used by traditional peoples in the treatment of diabetes and associated wound healing. In our previous study we had found that the ethanolic extract of leaves of Cinnamomum tamala is most active in treatment of wound healing in diabetic rats. The aim of our study was to find the active isolated eugenol from ethyl acetate soluble fraction of ethanolic extract of C. tamala leaves responsible for wound healing activity in diabetic rats. The Wistar albino rats were made diabetic by single i.p. injection of Streptozotocin (60 mg/kg). The excision, incision and dead space wound were created on back side of rats. The eugenol isolated from ethyl acetate soluble fraction of ethanolic extract of leaves of Cinnamomum tamala was applied topically in excision wound model while in incision and dead space wound model the eugenol isolated from ethyl acetate soluble fraction (100 mg/kg) was give orally for 16 days. In the excision wound model the wound area and day of epithelisation both were significantly decreased eugenol isolated from ethyl acetate soluble fraction treated rats. In incision wound model the significantly higher tensile strength was observed in rats treaded orally with eugenol from ethyl acetate soluble fraction.There were significant increase in weight of wet & dry granulation tissue with increased amount of hydroxyproline, collagen and elastin was observed in eugenol treated rats by ethyl acetate soluble fraction. The results suggested that the eugenol isolated from ethyl acetate soluble fraction of ethanolic extract of leaves of Cinnamomum tamala can be beneficial in treatment of wound healing in diabetic rats.
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