DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF BRIMONIDINE TARTRATE AS BULK DRUG AND IN OPHTHALMIC FORMULATION

  • S S Mandan MET’s Institute of Nashik Shirpur, India (MH)
  • A V Nerpagar MET’s Institute of Nashik Shirpur, India (MH)
  • U D Laddha MET’s Institute of Nashik Shirpur, India (MH)

Abstract

The optimized reverse phase high performance liquid chromatographic method was developed for estimation of Brimonidine Tartrate in bulk drug and pharmaceutical dosage form. Chromatography was performed on Kromasil C 18 ( 250 mm X 4.6 mm i.d. , 5 μm particle size) column with mobile phase citric acid monohydrate buffer:water:methanol (30:50:20 v/v/v) and  pH 3 was maintained by using triethylamine. The flow rate was 1.0 ml/min. Elute was detected at 246 nm and it effectively separated at Retention Time of 5.96 min. The LOD and LOQ was 1.47 and 4.47 μg/ml respectively. A linear response was observed over the concentration range 40-80 μg/ml for Brimonidine Tartrate. Thus the proposed HPLC method was found accurate, specific, precise, robust and reproducible.

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Author Biographies

S S Mandan, MET’s Institute of Nashik Shirpur, India (MH)

MET’s Institute of Nashik Shirpur, India (MH)

A V Nerpagar, MET’s Institute of Nashik Shirpur, India (MH)

MET’s Institute of Nashik Shirpur, India (MH)

U D Laddha, MET’s Institute of Nashik Shirpur, India (MH)

MET’s Institute of Nashik Shirpur, India (MH)

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How to Cite
Mandan, S., Nerpagar, A., & Laddha, U. (2017). DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF BRIMONIDINE TARTRATE AS BULK DRUG AND IN OPHTHALMIC FORMULATION. Journal of Drug Delivery and Therapeutics, 7(7), 146-159. https://doi.org/10.22270/jddt.v7i7.1616