DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR ESTIMATION OF BRIMONIDINE TARTRATE AS BULK DRUG AND IN OPHTHALMIC FORMULATION
The optimized reverse phase high performance liquid chromatographic method was developed for estimation of Brimonidine Tartrate in bulk drug and pharmaceutical dosage form. Chromatography was performed on Kromasil C 18 ( 250 mm X 4.6 mm i.d. , 5 Î¼m particle size) column with mobile phase citric acid monohydrate buffer:water:methanol (30:50:20 v/v/v) andÂ pH 3 was maintained by using triethylamine. The flow rate was 1.0 ml/min. Elute was detected at 246 nm and it effectively separated at Retention Time of 5.96 min. The LOD and LOQ was 1.47 and 4.47 Î¼g/ml respectively. A linear response was observed over the concentration range 40-80 Î¼g/ml for Brimonidine Tartrate. Thus the proposed HPLC method was found accurate, specific, precise, robust and reproducible.
2. Phogat A, Kumar M, Mahadevan N, Simultaneous estimation of Brimonidine Tartrate and Timolol Maleate in Nanoparticles Formulation by RP-HPLC, Int J Recent Adv Pharm Res, 2011, 3, 31-36.
3. Jiang S, Chappa A, Proksch J, A rapid and sensitive LC/MS/MS assay for quantitation of brimonidine in ocular fluid and tissue , J Chromatogr B Anal Technol Biomed Life Sci, 2009, 3, 107-114.
4. Sethi PD, Quantitative analysis of pharmaceutical formulation, HPLC, 2011, 1, 38-70.
5. ICH Harmonised Tripartite Guideline Validation of Analytical Procedures, Text and Methodology, 2005, Q2, R1.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).