Evaluation of spermatic DNA fragmentation in smoking normospermic infertile individuals
Abstract
Aim: In approximately 20% of infertile couples, the male factor is the only main cause. In recent years, it has been hypothesized that sperm DNA integrity may be a better indicator than routine semen analysis. Although the effects of smoking on male infertility have not yet been proven, smoking is considered a reasonable risk factor for infertility. In our study, we aimed to evaluate DNA damage in normospermic infertile men who smoke and do not smoke using the acridine orange method.
Material and Methods: This study was conducted on semen samples from 50 male cases, 25 of whom were smokers and non-smokers, who met the infertility criteria and applied to the urology clinic of Dicle University Medical Faculty Hospital. Microscopic examinations and morphological evaluations were performed in accordance with the WHO 2010 criteria. The acridine orange method was applied to evaluate DNA fragmentation and DNA fragmentations were calculated. Statistical analysis of the obtained data was performed using the Mann-Whitney U Test.
Results: In our study, in the smoking group, age was significantly associated with the duration of infertility, concentration with total motile sperm count, total sperm count with total motile sperm count, and amorphous head anomaly (p<0.01). In the non-smoking group, age and infertility were significantly associated with the duration, volume and total count, concentration and total count, total motile sperm count, and DNA fragmentation (p<0.01). However, there was no statistical difference between the smoking and non-smoking groups in terms of semen parameters and DNA fragmentation (p>0.05).
Conclusion: In order to obtain more reliable results, it is thought that large-scale studies are needed in the same groups and in the general population.
Keywords: sperm, infertility, DNA fragmentation, smoking
Keywords:
sperm, infertility, DNA fragmentation, smokingDOI
https://doi.org/10.22270/jddt.v14i11.6893References
1. SÇ Duran, M Akkuş, E Duran, F Aşır,, et al. Immunohistochemical Analysis of Sperm Aquaporin-3 Expression in Men with Varicocele. Journal of Drug Delivery and Therapeutics, 2024;14(4):81-85. https://doi.org/10.22270/jddt.v14i4.6569
2. Koyuncu H. Methods for the determination of sperm DNA damage.Turk Urol Sem. 2011;2:18-23. https://doi.org/10.5152/tus.2011.04
3. Saleh RA, Agarwal A, Sharma RK, Nelson DR, Thomas AJ. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: a prospective study. Fertil Steril. 2002;78(3):491-499. https://doi.org/10.1016/S0015-0282(02)03294-6 PMid:12215323
4. Schulte RT, Ohl DA, Sigman M, Smith GD. Sperm DNA damage in male infertility: etiologies, assays, and outcomes. J Assist Reprod Genet. 2010;27(1):3-12. https://doi.org/10.1007/s10815-009-9359-x PMid:20012685 PMCid:PMC2826626
5. Liu K, Mao X, Pan F, Chen Y, An R. Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART. Sci Rep. 2023;13(1):2717. Published 2023 Feb 15. https://doi.org/10.1038/s41598-023-28765-z PMid:36792684 PMCid:PMC9931767
6. Özgür K, IĢıkoglu M, Seleker M, Dönmez L. Semen quality of smoking and non-smoking men in infertile couples in a Turkish population. Arch Gynecol Obstet. 2005;271(2):109-112. https://doi.org/10.1007/s00404-003-0572-z PMid:14685893
7. Colagar AH, Jorsaraee GA, Marzony ET. Cigarette smoking and the risk of male infertility.Pak J Biol Sci. 2007;10: 3870-3874. https://doi.org/10.3923/pjbs.2007.3870.3874 PMid:19090244
8. Koyuncu H. Methods for the determination of sperm DNA damage.Turk Urol Sem. 2011;2:18-23. https://doi.org/10.5152/tus.2011.04
9. Collodel G, Capitani S, Pammolli A,Giannerini V, Geminiani M, Moretti E.Semen quality of male idiopathic infertile smokers and nonsmokers: an ultrastructural study. J Androl. 2010;31:108-13. https://doi.org/10.2164/jandrol.109.007773 PMid:19745220
10. Sepaniak S, Forges T, Gerard H, Foliguet B, Bene MC, Monnier-Barbarino P. The influence of cigarette smoking on human sperm quality and DNA fragmentation. Toxicol. 2006;223(1-2):54-60. https://doi.org/10.1016/j.tox.2006.03.001 PMid:16621218
11. WHO Laboratory Manual for The Examination and Processing of Human Semen, 5th edition, WHO, 2010.
12. Z. Çankırı, M. Akkuş, E. Yildizhan, D. Akıncı, F. Aşır, O. Dede, and M. Afşin, "Investigation of Globozoospermia's Morphological Structure and DNA Fragmentation in Oligozoospermia Cases in Infertile Males", MEJS, vol. 2022;8(1):56-63, https://doi.org/10.51477/mejs.1095136
13. Makker K, Agarwal A, Sharma R. Oxidative stress & male infertility. Indian J Med Res. 2009;129(4):357-67.
14. Koyuncu H. Methods for the determination of sperm DNA damage.Turk Urol Sem. 2011;2:18-23. https://doi.org/10.5152/tus.2011.04
15. Collodel G, Capitani S, Pammolli A,Giannerini V, Geminiani M, Moretti E.Semen quality of male idiopathic infertile smokers and nonsmokers: an ultrastructural study. J Androl. 2010;31:108-13. https://doi.org/10.2164/jandrol.109.007773 PMid:19745220
16. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health. 2014 Apr;32(1):1-17. https://doi.org/10.5534/wjmh.2014.32.1.1 PMid:24872947 PMCid:PMC4026229
17. Martini AC, Molina RI, Estofan D, Senestrari D, Fiol de Cuneo M. Effects of alcohol and cigarette consumption on human seminal quality. Fertil Steril. 2004;82(2):374 https://doi.org/10.1016/j.fertnstert.2004.03.022 PMid:15302286
18. Aşır, F., Duran, S. Ç., Afşin, M., Duran, E., Korak, T., & Şahin, F. Investigation of Vitamin D Levels in Men with Suspected Infertility. Life, 2024;14(2):273. https://doi.org/10.3390/life14020273 PMid:38398783 PMCid:PMC10889942
Published
Abstract Display: 88 
PDF Downloads: 31 PDF Downloads: 3 How to Cite
Issue
Section
Copyright (c) 2024 Mehmet Uğur Karabat , Murat Akkuş , Şehmus Kaplan , Muhyiddin Sancar , Esranur Atiç
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.