In vitro Cyclooxygenase inhibitory activity and GC-MS profiling of bioactive compounds in Bauhinia racemosa Lam.
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs) are a common treatment for chronic pain and inflammation. While NSAIDs have been shown to reduce inflammation and pain associated with them, they have an array of side effects. Researchers investigated the COX inhibitory action of NSAIDs. They revealed that there are two distinct COX enzymes: COX-1 and COX2. Developing COX inhibitors has remained critical for producing innovative and safe anti-inflammatory drugs. Bauhinia racemosa Lam belonging to the family Fabaceae has a wide range of medicinal properties. The plant is used to treat various diseases in traditional medicine, but there is less knowledge based on clinical efficacy as an anti-inflammatory agent. In the present study Bauhinia racemosa Lam hydroalcoholic extract were screened for COX inhibition and GC-MS analysis was done to screen phytochemicals. The results obtained were found to inhibit 89% of COX activity significantly as compared to standard drug (Diclofenac sodium). GC-MS analysis revealed a total of ten potent bioactive compounds. The samples were identified by comparing retention time and peak area to literature and interpreting mass spectra. Results observed from the study suggest that the hydroalcoholic extract of Bauhinia racemosa Lam possess anti-inflammatory activity and can be utilized as an alternate source for NSAIDs.
Keywords: COX, Bauhinia racemosa Lam, Anti-inflammatory, NSAIDs.
Keywords:
COX, Bauhinia racemosa Lam, Anti-inflammatory, NSAIDsDOI
https://doi.org/10.22270/jddt.v14i10.6847References
Joray MB, Trucco LD, González ML, Napal GN, Palacios SM, Bocco JL, Carpinella MC. Antibacterial and cytotoxic activity of compounds isolated from Flourensia oolepis. Evidence‐Based Complementary and Alternative Medicine. 2015;2015(1):912484. https://doi.org/10.1155%2F2015%2F912484
Rainsford K. Anti-inflammatory drugs in the 21st century. Inflammation in the pathogenesis of chronic diseases: the COX-2 controversy. 2007 Jan 1:3-27. https://doi.org/10.1007/1-4020-5688-5_1
Yedgar S, Krimsky M, Cohen Y, Flower RJ. Treatment of inflammatory diseases by selective eicosanoid inhibition: a double-edged sword?. Trends in pharmacological sciences. 2007 Sep 1;28(9):459-64. https://doi.org/10.1016/j.tips.2007.07.005
Honda T, Segi-Nishida E, Miyachi Y, Narumiya S. Prostacyclin-IP signaling and prostaglandin E2-EP2/EP4 signaling both mediate joint inflammation in mouse collagen-induced arthritis. The Journal of experimental medicine. 2006 Feb 20;203(2):325-35. https://doi.org/10.1084%2Fjem.20051310
Mabuchi T, Kojima H, Abe T, Takagi K, Sakurai M, Ohmiya Y, Uematsu S, Akira S, Watanabe K, Ito S. Membrane-associated prostaglandin E synthase-1 is required for neuropathic pain. Neuroreport. 2004 Jun 28;15(9):1395-8. https://doi.org/10.1097/01.wnr.0000129372.89000.31
Backlund MG, Mann JR, DuBois RN. Mechanisms for the prevention of gastrointestinal cancer: the role of prostaglandin E2. Oncology. 2005 Sep 1;69(Suppl. 1):28-32. https://doi.org/10.1159/000086629
Hahn EL, He LK, Gamelli RL. Prostaglandin E2 synthesis and metabolism in burn injury and trauma. Journal of Trauma and Acute Care Surgery. 2000 Dec 1;49(6):1147-54. https://doi.org/10.1097/00005373-200012000-00033
Subbaramaiah K, Yoshimatsu K, Scherl E, Das KM, Glazier KD, Golijanin D, Soslow RA, Tanabe T, Naraba H, Dannenberg AJ. Microsomal prostaglandin E synthase-1 is overexpressed in inflammatory bowel disease: evidence for involvement of the transcription factor Egr-1. Journal of Biological Chemistry. 2004 Mar 26;279(13):12647-58. https://doi.org/10.1074/jbc.m312972200
Torres CA, Zamora CM, Nuñez MB, Gonzalez AM. In vitro antioxidant, antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae. Journal of integrative medicine. 2018 Jul 1;16(4):255-62. https://doi.org/10.1016/j.joim.2018.04.009
Desai SJ, Prickril B, Rasooly A. Mechanisms of phytonutrient modulation of cyclooxygenase-2 (COX-2) and inflammation related to cancer. Nutrition and cancer. 2018 Apr 3;70(3):350-75. https://doi.org/10.1080%2F01635581.2018.1446091
Lee JL, Mukhtar H, Bickers DR, Kopelovich L, Athar M. Cyclooxygenases in the skin: pharmacological and toxicological implications. Toxicology and applied pharmacology. 2003 Nov 1;192(3):294-306. https://doi.org/10.1016/s0041-008x(03)00301-6
Vane J. Towards a better aspirin. Nature. 1994 Jan 20;367(6460). https://doi.org/10.1038/367215a0
Mukherjee D, Nissen SE, Topol EJ. Risk of cardiovascular events associated with selective COX-2 inhibitors. Jama. 2001 Aug 22;286(8):954-9. https://doi.org/10.1001/jama.286.8.954
Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essay. 2007 May 1;2(5):163-6.
Promprom W, Chatan W. GC-MS Analysis and antioxidant activity of Bauhinia nakhonphanomensis leaf ethanolic extract. Pharmacognosy Journal. 2017;9(5). http://dx.doi.org/10.5530/pj.2017.5.105
Yadav RN, Agarwala M. Phytochemical analysis of some medicinal plants. Journal of phytology. 2011 Dec 14;3(12).
Mythili K, Umamaheswara Reddy C, Chamundeeswari D, Manna PK. GC-MS analysis of phytocomponents and in vitro inhibitory effects of Calanthe triplicata. Journal of Natural Products. 2013;6:141-6.
Panda P, Das D, Dash P, Ghosh G. Therapeutic potential of Bauhinia racemosa-a mini review. Int J Pharm Sci Rev Res. 2015;32(2):169-79.
Akhtar AH, Ahmad KU. Anti-ulcerogenic evaluation of the methanolic extracts of some indigenous medicinal plants of Pakistan in aspirin-ulcerated rats. Journal of Ethnopharmacology. 1995 Apr 1;46(1):1-6. https://doi.org/10.1016/0378-8741(94)01220-t
Kumar RS, Sivakumar T, Sunderam RS, Gupta M, Mazumdar UK, Gomathi P, Rajeshwar Y, Saravanan S, Kumar MS, Murugesh K, Kumar KA. Antioxidant and antimicrobial activities of Bauhinia racemosa L. stem bark. Brazilian journal of medical and biological research. 2005;38:1015-24. https://doi.org/10.1590/s0100-879x2005000700004
Jain R, Saxena U, Rathore K, Jain SC. Bioactivities of polyphenolics from the roots of Bauhinia racemosa. Archives of pharmacal research. 2008 Dec;31:1525-9. https://doi.org/10.1007/s12272-001-2145-7
Borikar VI, Jangde CR, Philip P, Rekhe DS, Atole SK. Study of antipyretic activity of Bauhinia racemosa Lam in rats. Veterinary world. 2009 Jun 1;2(6):215.
Zarghi A, Arfaei S. Selective COX-2 inhibitors: a review of their structure-activity relationships. Iranian journal of pharmaceutical research: IJPR. 2011;10(4):655.
Akinloye OA, Metibemu DS, Akinloye DI, Onigbinde SB, Olaosebikan IA, Florence O, Damilola B, Bolarinwa OA, Olubunmi O. Flavanones from Sorghum bicolor selectively inhibit COX-2: in-silico and in-vivo validation. Egyptian Journal of Medical Human Genetics. 2019 Dec;20:1-5. https://doi.org/10.1186/s43042-019-0029-y
Attiq A, Jalil J, Husain K, Ahmad W. Raging the war against inflammation with natural products. Frontiers in pharmacology. 2018 Sep 7;9:976. https://doi.org/10.3389/fphar.2018.00976
Jayaprakasam B, Zhang Y, Nair MG. Tumor cell proliferation and cyclooxygenase enzyme inhibitory compounds in Amaranthus tricolor. Journal of Agricultural and Food Chemistry. 2004 Nov 17;52(23):6939-43. https://doi.org/10.1021/jf048836z
Starlin T, Prabha PS, Thayakumar BK, Gopalakrishnan VK. Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora. Bioinformation. 2019;15(6):425. https://doi.org/10.6026%2F97320630015425
Pearson DC, Jourd'Heuil D, Meddings JB. The anti-oxidant properties of 5-aminosalicylic acid. Free Radical Biology and Medicine. 1996 Jan 1;21(3):367-73. https://doi.org/10.1016/0891-5849(96)00031-7
Punchard NA, Greenfield SM, Thompson RP. Mechanism of action of 5‐arninosalicylic acid. Mediators of inflammation. 1992;1(3):151-65. https://doi.org/10.1155%2FS0962935192000243
TA Abdel-Wareth M, A Ghareeb M, S Abdel-Aziz M, M El-Hagrassi A. Snailicidal, antimicrobial, antioxidant and anticancer activities of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus fungal extracts. Egyptian Journal of Aquatic Biology and Fisheries. 2019 Apr 25;23(2):195-212. https://dx.doi.org/10.21608/ejabf.2019.30550
More K, Tayade S, Gawande P, Manik S, Shelke D. Antioxidant and antimicrobial potential of Canavalia gladiata (Jacq.) DC. leaves and seeds: GC-MS based metabolic profiling. Indian Journal of Natural Products and Resources (IJNPR)[Formerly Natural Product Radiance (NPR)]. 2022 Jul 26;13(2):163-9. http://dx.doi.org/10.56042/ijnpr.v13i2.47499
Chelkar M, Harke M, Pandiyan A. Identification of chemical compounds from the ethanolic extract of Bauhinia racemose Lam. Bark by GC-MS analysis, Pharma Innov J.2020 Nov13; 9(11):11-3.
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