An assessment of various disinfectants using the Kirby-Bauer Method with disc diffusion to determine their effectiveness against locally isolated pathogens

  • Muhammad Danish Mehmood The University of Lahore, Lahore
  • Sawaira Sabir University of Veterinary & Animal Sciences, Lahore
  • Muhammad Usman Ghani Ottoman Pharma Immuno Division, Lahore
  • Huma Anwar ul-Haq Ottoman Pharma Immuno Division, Lahore
  • Rauf Khalid Ottoman Pharma Immuno Division, Lahore
  • Nadeem Sharif University of Veterinary & Animal Sciences, Lahore

Abstract

This study aimed to assess the effectiveness of various disinfectants against locally isolated pathogens using the Kirby-Bauer Method with disc diffusion. Indigenous strains of Salmonella typhimurium, E. coli, Campylobacter, Citrobacter freundii, and Staphylococcus aureus were obtained from the bacterial depository bank and sub-cultured on their respective selective media. A 0.5 McFarland Turbidity Standard was prepared, and eight commercially available disinfectants were tested for efficacy. The results of this study will inform the selection of appropriate disinfectants for use in preventing the spread of disease in various settings, including home sanitation, healthcare, and industrial manufacturing processes. The disinfectants used in this study produced different results against the targeted pathogens. Hydrogen peroxide and Formalin produced larger zones of inhibition, while Povidone Iodine and Hydrochloric acid produced intermediate zones. Ethanol, Methanol, and Dettol produced smaller .zones of inhibition. Benzalkonium Chloride was effective only against S. aureus, while all other indigenous isolates resisted it.


Keywords: Disinfectants, Sal. typhimurium, E.coli, Campylobacter, Citrobacter freundii, Staphylococcus auerus,  Antibiotic sensitivity test

Keywords: Disinfectants, Sal. typhimurium, E.coli, Campylobacter, Citrobacter freundii, Staphylococcus auerus, Antibiotic sensitivity test

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Author Biographies

Muhammad Danish Mehmood, The University of Lahore, Lahore

The University of Lahore, Lahore

Sawaira Sabir, University of Veterinary & Animal Sciences, Lahore

University of Veterinary & Animal Sciences, Lahore 

Muhammad Usman Ghani, Ottoman Pharma Immuno Division, Lahore

Ottoman Pharma Immuno Division, Lahore

Huma Anwar ul-Haq, Ottoman Pharma Immuno Division, Lahore

Ottoman Pharma Immuno Division, Lahore

Rauf Khalid, Ottoman Pharma Immuno Division, Lahore

Ottoman Pharma Immuno Division, Lahore

Nadeem Sharif, University of Veterinary & Animal Sciences, Lahore

University of Veterinary & Animal Sciences, Lahore 

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Mehmood MD, Sabir S, Ghani MU, Anwar ul-Haq H, Khalid R, Sharif N. An assessment of various disinfectants using the Kirby-Bauer Method with disc diffusion to determine their effectiveness against locally isolated pathogens. JDDT [Internet]. 15Jun.2024 [cited 17Jul.2024];14(6):143-9. Available from: https://jddtonline.info/index.php/jddt/article/view/6612