Cytotoxicity and antioxidant potential of dichloromethane and ethyl acetate extracts of Morinda morindoïdes leaves

  • Aya Honorine TANOH Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire
  • Kouassi Elisée KPOROU Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire
  • Anaïs BRION Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France
  • Cendrine SEGUIN Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France
  • Antoine KITCHLER Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France
  • Benoit FRISCH Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France
  • Sylvie FOURNEL Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Abstract

The incidence of cancer and related mortality are on the rise worldwide, and this requires the search for new plant-based medicines. In this study, the antioxidant potential and cytotoxic activity against HCT-116 cell line were evaluated. Dichloromethane and ethyl acetate extracts of M. morindoïdes were prepared by maceration and evaluated for some biological properties. Cytotoxicity was assessed according to MTS method on the cancer line HCT-116 (colorectal carcinoma) using doxorubicin as reference. Antioxidant potential was carried out by DPPH and ABTS tests. Tri-phytochemistry was carried out from coloring and precipitation tests. On HCT-116 cell line, extracts demonstrated dose-response activity. The lowest IC50 value was obtained with the acetate extract with an IC50= 16.38 ± 0.2 µg/mL. IC50 values for antioxidant activities were evaluated at 39.70 ± 1.7 µg/mL and at 57.65 ± 4.3µg/mL by DPPH and ABTS test respectilly. Finally, phytochemical screening showed presence of polyphenols, flavonoids and quinone. M. morindoides ethyl acetate extract showed the best cytotoxicity and antioxidant activities. Therefore, it should be an interesting source for discovery of new compounds for colorectal cancer treatment.


Keywords: cytotoxicity, cancer cells, antioxidant, Morinda morindoides.

Keywords: cytotoxicity, cancer cells, antioxidant, Morinda morindoides

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Author Biographies

Aya Honorine TANOH, Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire

Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire

Kouassi Elisée KPOROU, Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire

Excellence Group of Research on Products of Traditional Pharmacopoeia (GeRProPhaT, Daloa Côte D’Ivoire), Agrovalorization Laboratory, Jean Lorougnon Guede University, BP 150 Daloa, Côte d’Ivoire

Anaïs BRION, Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Cendrine SEGUIN, Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Antoine KITCHLER, Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Benoit FRISCH, Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Sylvie FOURNEL, Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

Laboratoire de Conception et Application de Molécules Bioactives- Equipe 3bio Bureau F320, UMR 7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, 74 Route du Rhin- BP 60024- 67401 Illkirch Cédex- France

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TANOH AH, KPOROU KE, BRION A, SEGUIN C, KITCHLER A, FRISCH B, FOURNEL S. Cytotoxicity and antioxidant potential of dichloromethane and ethyl acetate extracts of Morinda morindoïdes leaves. JDDT [Internet]. 15Jun.2024 [cited 17Jul.2024];14(6):98-04. Available from: https://jddtonline.info/index.php/jddt/article/view/6571