Phytochemical Analysis, Antioxidant and Anti Hyperlipidemic Activity of Nyctanthes arbor-tristis in Male Wistar Rats

  • Tanya Jain Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
  • Arpit Shrivastava Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
  • Prateek Jain Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
  • Sunil Jain Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
  • Harshita Jain Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001

Abstract

The current study's objective was to assess the antihyperlipidemic, in vitro antioxidant, and qualitative and quantitative phytochemical analysis of Nyctanthes arbor-tristis flowers hydroalcoholic extract at 200 and 400 mg/kg in rats that were made hyperlipidemic by a high-fat diet. The Folins Ciocalteau reagent and the aluminium chloride method were used for the quantitative measurement of flavonoids and phenolics, respectively. Through phytochemical investigation, alkaloids, carbohydrates, glycosides, tannins, phenolic compounds, triterpenoids, and steroids were found in the hydroalcoholic extracts of N. arbor-tristis flowers.  The hydroalcoholic extract flowers had a total phenolic content of 126.000 mg/gm, which was followed by flavonoids at 103.104 mg/gm. Hydroalcoholic extracts of flowers showed concentration-dependent antioxidant activity in vitro against DPPH and superoxide radical scavenging test technique. In this investigation, rats were given a high-fat diet as part of an experimental induction procedure. By lowering LDL and VLDL cholesterol levels and raising HDL levels, the HANAT therapy and the medication atorvastatin dramatically reduce body weight. grew When hydroalcoholic flower extracts were given orally to animals that had been forced to become hyperlipidaemic due to a high cholesterol diet, the levels of HDL cholesterol, triglycerides, low density lipoproteins, and very low-density lipoproteins were all much lower. The p value (p<0.001) indicated that the results were significant. The triton-induced study results indicate that animals treated with HANAT at dose levels of 200 mg/kg and 400 mg/kg had considerably lower blood lipid parameters (p<0.01) than the control group; however, the 400 mg/kg of HANAT group animals showed significantly higher serum lipid parameters (p<0.001) than the control group. Additionally, a higher HDL level was noted at this time.


Keywords: Nyctanthes arbor-tristis, Physicochemical analysis, Antioxidant activity, DPPH. Antihyperlipidemic activity

Keywords: Nyctanthes arbor-tristis, Physicochemical analysis, Antioxidant activity, DPPH, Antihyperlipidemic activity

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Author Biographies

Tanya Jain, Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Arpit Shrivastava, Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Prateek Jain, Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Sunil Jain, Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001
Harshita Jain, Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001

Adina Institute of Pharmaceutical Science, Lahdara, Sagar, MP, 470001

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Jain T, Shrivastava A, Jain P, Jain S, Jain H. Phytochemical Analysis, Antioxidant and Anti Hyperlipidemic Activity of Nyctanthes arbor-tristis in Male Wistar Rats. JDDT [Internet]. 15Jun.2024 [cited 17Jul.2024];14(6):74-6. Available from: https://jddtonline.info/index.php/jddt/article/view/6570