Immunohistochemical Analysis of Sperm Aquaporin-3 Expression in Men with Varicocele

  • Senem Çetin Duran Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Murat Akkuş Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Enis Duran Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey
  • Fırat Aşır Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Nilüfer Dönmezdil Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Eda Yıldızhan Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Dilara Konk Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey
  • Kazım Uçkan Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey

Abstract

Objective: The primary objective of this study is to detect potential alterations in sperm cells in cases of varicocele using immunohistochemical methods.


Materials and Methods: In this study, semen samples obtained with consent from infertile males aged between 25-40 years were analyzed for the presence of varicocele and other determined factors, and were divided into two groups: a normozoospermic control group and a group diagnosed with varicocele. Direct swim-up techniques were utilized to separate spermatozoa with high motility. Immunohistochemical staining methods were employed to visualize protein expression, and the results were photographed at high magnification for documentation.


Results: This research was conducted on sperm samples collected from males diagnosed with varicocele and from a healthy control group. The findings suggest that the presence of aquaporin-3 protein is associated with low or negative expression in sperm cells in the presence of varicocele. Immunohistochemical analyses have revealed a reduced or absent expression of aquaporin 3 in patients with varicocele compared to healthy individuals.


Conclusion: The results of this study clearly demonstrate the adverse effects of varicocele on the structural and functional integrity of spermatozoa. Given that aquaporin-3 protein is critical for the motility of spermatozoa, the reduced expression or absence of this protein sheds light on the molecular mechanisms of male infertility.


Keywords: Sperm DNA fragmentation, immunohistochemistry, Varicocele, protein expression, male infertility, aquaporin-3.

Keywords: Sperm DNA fragmentation, immunohistochemistry, Varicocele, protein expression, male infertility, aquaporin-3

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Author Biographies

Senem Çetin Duran, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Murat Akkuş, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Enis Duran, Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey

Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey

Fırat Aşır, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Nilüfer Dönmezdil, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Eda Yıldızhan, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Dilara Konk, Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Department of Histology and Embryology, Medical Faculty, Dicle University, Diyarbakır, Turkey

Kazım Uçkan, Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey

Department of Gynecology and Obstetrics, Medical Faculty, Van Yüzüncü Yıl University, Van, Turkey

References

1. Esteves SC, Agarwai A. The azoospermic male: current knowledge and future perspectives. Clinics (Sao Paulo). 2013;68 Suppl 1(Suppl 1):1-4. https://doi.org/10.6061/clinics/2013(Sup01)01 PMid:23503949
2. Jensen CFS, Østergren P, Dupree JM, Ohl DA, Sønksen J, Fode M. Varicocele and male infertility. Nature Reviews Urology. 2017;14(9):523-33. https://doi.org/10.1038/nrurol.2017.98 PMid:28675168
3. Lundy SD, Sabanegh ES, Jr. Varicocele management for infertility and pain: A systematic review. Arab J Urol. 2018;16(1):157-70. https://doi.org/10.1016/j.aju.2017.11.003 PMid:29713547 PMCid:PMC5922006
4. Sheehan MM, Ramasamy R, Lamb DJ. Molecular mechanisms involved in varicocele-associated infertility. J Assist Reprod Genet. 2014;31(5):521-6. https://doi.org/10.1007/s10815-014-0200-9 PMid:24643631 PMCid:PMC4016365
5. Kang C, Punjani N, Lee RK, Li PS, Goldstein M. Effect of varicoceles on spermatogenesis. Semin Cell Dev Biol. 2022;121:114-124. https://doi.org/10.1016/j.semcdb.2021.04.005 PMid:33965333
6. Kantartzi PD, Goulis ChD, Goulis GD, Papadimas I. Male infertility and varicocele: myths and reality. Hippokratia. 2007 Jul;11(3):99-104.
7. Pellavio G, Laforenza U. Human sperm functioning is related to the aquaporin-mediated water and hydrogen peroxide transport regulation. Biochimie. 2021;188:45-51. https://doi.org/10.1016/j.biochi.2021.05.011 PMid:34087390
8. Yeung C, Callies C, Tüttelmann F, Kliesch S, Cooper T. Aquaporins in the human testis and spermatozoa-identification, involvement in sperm volume regulation and clinical relevance. International journal of andrology. 2010;33(4):629-41. https://doi.org/10.1111/j.1365-2605.2009.00998.x PMid:19840149
9. Moretti E, Terzuoli G, Mazzi L, Iacoponi F, Collodel G. Immunolocalization of aquaporin
7 in human sperm and its relationship with semen parameters.
10. Cayan S, Kadioglu A, Orhan I, Kandirali E, Tefekli A, Tellaloglu S. The effect of microsurgical varicocelectomy on serum follicle stimulating hormone, testosterone and free testosterone levels in infertile men with varicocele. BJU Int. 1999;84(9):1046-9. https://doi.org/10.1046/j.1464-410x.1999.00353.x PMid:10571633
11. Seifer I, Amat S, Delgado-Viscogliosi P, Boucher D, Bignon YJ. Screening for microdeletions on the long arm of chromosome Y in 53 infertile men. Int J Androl. 1999;22(3):148-54. https://doi.org/10.1046/j.1365-2605.1999.00161.x PMid:10367234
12. Bonilla‐Correal S, Noto F, Garcia‐Bonavila E, Rodríguez‐Gil J, Yeste M, Miró J. First evidence for the presence of aquaporins in stallion sperm. Reproduction in domestic animals. 2017;52:61-4. https://doi.org/10.1111/rda.13059 PMid:29052325
13. Prieto‐Martínez N, Vilagran I, Morató R, Rivera del Álamo MM, Rodríguez‐Gil JE, Bonet S, Yeste M. Relationship of aquaporins 3 (AQP 3), 7 (AQP 7), and 11 (AQP 11) with boar sperm resilience to withstand freeze-thawing procedures. Andrology. 2017;5(6):1153-64. https://doi.org/10.1111/andr.12410 PMid:28941027
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Duran S Çetin, Akkuş M, Duran E, Aşır F, Dönmezdil N, Yıldızhan E, Konk D, Uçkan K. Immunohistochemical Analysis of Sperm Aquaporin-3 Expression in Men with Varicocele. JDDT [Internet]. 15Apr.2024 [cited 24Jun.2024];14(4):81-5. Available from: https://jddtonline.info/index.php/jddt/article/view/6569

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