Gallic Acid and Doxorubicin Treatment HeLa cell lines by MTT assay

Authors

Abstract

Objective: Cervical cancer, as it is medically known, occurs in the cells in the lower part of the uterus called the cervix and is one of the most common gynecological cancers in the world. Hormonal and genetic factors play a major role in the development of endometrial cancer. Chemotherapeutic drugs are very effective in treatment. However, serious side effects and the development of drug resistance limit the use of these drugs. In our study, the cytotoxic effects of Gallic acid (GA), which is found in many popular foods, on HeLa cells were investigated.

Methods: In our study, different doses of GA and Dox were applied to the cells for 48, 72 h and cytotoxicity levels were determined by the MTT method. All results were analyzed statistically.

Results: It was observed that cytotoxicity by MTT was at the highest level in the GA and Dox administered group. IC50 was determined and it was found that GA IC50 was 242.4 and Dox IC50 was 124.6 for 48 h.

Conclusion: As a result of the study, it was shown that the highest cytotoxic effect occurred with GA application and this may support Dox application with a synergistic effect.

Keywords: Servics cancer, Gallic acid, Doxorubicin, MTT

Keywords:

Servics cancer, Gallic acid, Doxorubicin, MTT

DOI

https://doi.org/10.22270/jddt.v14i2.6459

Author Biographies

Serap Mutlu Özçelik Otçu, Dicle University, Medical Faculty, Department of Histology and Embryology, Diyarbakır, Turkey

Dicle University, Medical Faculty, Department of Histology and Embryology, Diyarbakır, Turkey

Murat Akkuş, Dicle University, Medical Faculty, Department of Histology and Embryology, Diyarbakır, Turkey

Dicle University, Medical Faculty, Department of Histology and Embryology, Diyarbakır, Turkey

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Published

15-02-2024
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How to Cite

1.
Özçelik Otçu SM, Akkuş M. Gallic Acid and Doxorubicin Treatment HeLa cell lines by MTT assay. J. Drug Delivery Ther. [Internet]. 2024 Feb. 15 [cited 2025 Feb. 13];14(2):247-51. Available from: https://jddtonline.info/index.php/jddt/article/view/6459

How to Cite

1.
Özçelik Otçu SM, Akkuş M. Gallic Acid and Doxorubicin Treatment HeLa cell lines by MTT assay. J. Drug Delivery Ther. [Internet]. 2024 Feb. 15 [cited 2025 Feb. 13];14(2):247-51. Available from: https://jddtonline.info/index.php/jddt/article/view/6459

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