Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx

Authors

  • Judith Emery Kanemoto Ngomoyogoli Laboratory of Phytoprotection and Valorisation of Genetic Resources, The Biotechnology Centre, University of Yaoundé I, P.O. Box 3851, Messa-Yaoundé Cameroon https://orcid.org/0000-0001-8648-5026
  • Fils Armand Ella Department of Pharmacology, School of Clinical Medicine, Faculty of Health Sciences, University of the Free State, P.O. Box 3900, Bloemfontein, South Africa https://orcid.org/0000-0001-8648-5026
  • Stanley Olivier Kanemoto Laboratory of Applied Inorganic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon https://orcid.org/0000-0001-5968-1988
  • Thaddée Boudjeko Laboratory of Phytoprotection and Valorisation of Genetic Resources, The Biotechnology Centre, University of Yaoundé I, P.O. Box 3851, Messa-Yaoundé Cameroon https://orcid.org/0000-0002-1205-4255
  • Judith Laure Ngondi Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon
  • Nicolas Njintang Yanou Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454 Ngaoundéré, Cameroon https://orcid.org/0000-0002-8949-7150

Abstract

The hot-water extraction method has been employed to enhance the diffusion rate of heteroglycans, thereby augmenting the extraction efficiency. This study investigates the antioxidant activities and immunological properties of water-soluble polysaccharides derived from the Hibiscus sabdariffa calyx (HWPF and LWPF). These polysaccharides were isolated through two distinct extraction methods involving hot water followed by ethanol precipitation. The physicochemical characterization was conducted through a series of experiments encompassing monosaccharide composition, ultraviolet-visible spectrum analysis, and Fourier-transform infrared (FT-IR) spectroscopy. The evaluation of antioxidative properties employed various techniques, including DPPH and ABTS radical scavenging activities, Ferric Reducing Antioxidant Power, ferrous chelating power, total reducing power, and protection against DNA damage. The assessment of anti-inflammatory potential involved the inhibition of hemolytic activity induced by a hypotonic solution and heat. Furthermore, the impact of the polysaccharides on the production of pro-inflammatory cytokines was analyzed through Luminex assay and RTPCR assay in PBMCs and LPS-stimulated RAW 264.7 cells, respectively. The findings revealed that heteroglycans (LWPF and HWPF) exhibit a content of Ara-Man-Rha-GluA-GalA (41.2:17.2:15.7:9.2:8.8) and Ara-Glu-Rha-Xyl-GluA-Man (31.5:23.8:12.16:10.4:7:6) with molecular weights of approximately 4.5 KDa and 41.3 KDa, respectively. HWPF demonstrated robust metal chelating and scavenging activities. Additionally, each fraction exhibited substantial protection of DNA against damage induced by H2O2. LWPF was observed to significantly down-regulate the expression of pro-inflammatory cytokines IL-6 and COX-2 at both transcriptional and translational levels, along with inhibiting NO production. In summary, these results indicate that the choice of extraction methods influences both the structure and biological activities of heteroglycans, providing valuable insights into the structure-activity relationship.

Keywords: Hibiscus sabdariffa, heteroglycans, hot water extraction, physicochemical properties, Antioxidant activity, Pro-inflammatory cytokines.

Keywords:

Hibiscus sabdariffa, heteroglycans, hot water extraction, physicochemical properties, Antioxidant activity, Pro-inflammatory cytokines

DOI

https://doi.org/10.22270/jddt.v14i4.6436

Author Biographies

Judith Emery Kanemoto Ngomoyogoli, Laboratory of Phytoprotection and Valorisation of Genetic Resources, The Biotechnology Centre, University of Yaoundé I, P.O. Box 3851, Messa-Yaoundé Cameroon

Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Hyderabad Campus, jawahar Nagar, Sharmipet Mandal, Hyderabad, India

Fils Armand Ella, Department of Pharmacology, School of Clinical Medicine, Faculty of Health Sciences, University of the Free State, P.O. Box 3900, Bloemfontein, South Africa

Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Stanley Olivier Kanemoto, Laboratory of Applied Inorganic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Laboratory of Applied Inorganic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Thaddée Boudjeko, Laboratory of Phytoprotection and Valorisation of Genetic Resources, The Biotechnology Centre, University of Yaoundé I, P.O. Box 3851, Messa-Yaoundé Cameroon

Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Judith Laure Ngondi, Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Department of Biochemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon

Nicolas Njintang Yanou, Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454 Ngaoundéré, Cameroon

Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454 Ngaoundéré, Cameroon

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15-04-2024
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How to Cite

1.
Kanemoto Ngomoyogoli JE, Ella FA, Kanemoto SO, Boudjeko T, Ngondi JL, Njintang Yanou N. Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx. J. Drug Delivery Ther. [Internet]. 2024 Apr. 15 [cited 2025 Feb. 14];14(4):42-54. Available from: https://jddtonline.info/index.php/jddt/article/view/6436

How to Cite

1.
Kanemoto Ngomoyogoli JE, Ella FA, Kanemoto SO, Boudjeko T, Ngondi JL, Njintang Yanou N. Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of heteroglycans from Hibiscus sabdariffa calyx. J. Drug Delivery Ther. [Internet]. 2024 Apr. 15 [cited 2025 Feb. 14];14(4):42-54. Available from: https://jddtonline.info/index.php/jddt/article/view/6436

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