New Enriched Culture Media for Culturing Streptococcus pyogenes by using Spirulina Powder
Abstract
Background and Aim: Fastidious bacteria require special nutrients and growth factors to grow on enriched culture media. Many enriched culture media were developed for culturing and isolating fastidious bacteria in pure cultures. The present study investigates the ability of spirulina powder to support the fastidious bacteria growth in pure culture compared to ordinary enriched media, e.g., Blood agar and Chocolate agar.
Methods: Spirulina powder was used as a nutrient source with some additives to prepare different types of Spirulina media. Reference strain ATCC (19615) and three clinical isolates of Streptococcus pyogenes were examined for their growth on the developed candidate medium. Three formulations of spirulina powder were used: Medium 1, Medium 2, and Medium 3.
Results: All spirulina media supported the growth of streptococcus pyogenes and there was no significant difference in the morphology and cultural characteristics on the blood agar and chocolate agar media. Colonies size of streptococcus pyogenes were slightly smaller size on spirulina media.
Conclusion: Spirulina media is a possible candidate that can be used as enriched culture media for culturing and isolating fastidious bacteria such as Streptococcus pyogenes.
Keyword: Spirulina powder, enriched culture media, Streptococcus pyogenes.
Keywords:
Spirulina powder, enriched culture media, Streptococcus pyogenesDOI
https://doi.org/10.22270/jddt.v14i2.6365References
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