Evaluation of Effect of Rosmarinus officinalis L. on Ethylene Glycol Induced Kidney Stone in Rats
Abstract
The present study evaluation of effect of Rosmarinus officinalis L. on ethylene glycol induced kidney stone in rats showed that the ethanolic extract of Rosmarinus officinalis possess the diuretic activity and effective in the treatment of kidney stone. The activity was evaluated against ethylene glycol (0.75%) induced kidney stone in albino rats. Animals were divided into five groups, containing six animals in each. Group I serve as normal control and received regular rat food and drinking water ad libitum. Ethylene glycol (0.75%) in drinking water was feed to Groups II to V for induction of renal calculi for 28 days. Group III received standard anti-urolithiasis drug, cystone (750mg/kg body weight) from 15th to till 35th day. Group IV and V received ethanolic extract of Rosmarinus officinalis at dose of 100mg/kg and 200mg/kg respectively from 15th to till 35th day.
The serum creatinine level, serum urea level, urine calcium level, protein level in urine, serum uric acid was increased and serum calcium level, protein level in serum was decreased in all experimental groups except normal control group after ethylene glycol administration till day 14.
The animals treated on the day 14th with standard drug (Cystone 750mg/kg) and Rosmarinus officinalis extract (100mg/kg and 200mg/kg), were observed significant decreased serum creatinine level, serum urea level, urine calcium level, protein level in urine and serum uric acid and increased serum calcium level, protein level in serum compared to normal control group on the day 28th and 35th.
This indicates that the ethanolic extract of Rosmarinus officinalis (200mg/kg) have protective role against kidney stone.
Keywords: Kidney Stone, Ethanolic extract, Rosmarinus officinalis, Ethylene glycol, uric acid, creatinine.
Keywords:
Kidney Stone, Ethanolic extract, Rosmarinus officinalis, Ethylene glycol, uric acid, creatinineDOI
https://doi.org/10.22270/jddt.v13i11.6288References
Coe F. L.; Evan A. and Worcester E. "Kidney stone disease," Journal of Clinical Investigation, 2005; 115(10):2598-2608. https://doi.org/10.1172/JCI26662 PMid:16200192 PMCid:PMC1236703
Chhiber N.; Sharma M.; Kaur T. and Singla S. "Mineralization in health and mechanism of kidney stone formation," International Journal of Pharmaceutical Science Invention, 2014; 3(1):25-31.
Han H.; Segal A.M.; Seifter J.L. and Dwyer J.T. "Nutritional Management of Kidney Stones (Nephrolithiasis)". Clinical Nutrition Research, 2015; 4(3):137-152. https://doi.org/10.7762/cnr.2015.4.3.137 PMid:26251832 PMCid:PMC4525130
Skolarikos A.; Straub M. and Knoll T., "Metabolic evaluation and recurrence prevention for urinary stone patients: EAU guidelines," European Urology. 2015; 67(4):750-763. https://doi.org/10.1016/j.eururo.2014.10.029 PMid:25454613
Phillips, R.; Hanchanale, V. S.; Myatt, A.; Somani, B.; Nabi, G. &Biyani C. S. "Citrate salts for preventing and treating calcium containing kidney stones in adults" Cochrane Database of Systematic Reviews, 2015; (10)1-42. https://doi.org/10.1002/14651858.CD010057.pub2
Barbasa C.; Garciaa A.; Saavedraa L. and Muros M. "Urinary analysis of nephrolithiasis markers," Journal of Chromatography, 2002; 781(1-2):433-455. https://doi.org/10.1016/S1570-0232(02)00557-3 PMid:12450673
Griffith D.P.; "Struvite stones," Kidney International. 1978; 13(5):372-382. https://doi.org/10.1038/ki.1978.55 PMid:351265
Ngo T. C. and Assimos D. G. "Uric acid nephrolithiasis: recent progress and future directions," Reviews in Urology, 2007; 9:17-27.
Kumar S.B.N.; Kumar K.G.; Srinivasa V. and Bilal S. "A review on urolithiasis," International Journal of Universal Pharmacy and Life Sciences, 2012; 2(2):269-280.
Ahmed K.; Dasgupta P. and Khan M.S. "Cystine calculi: challenging group of stones," Postgraduate Medical Journal, 2006; 82(974):799-801. https://doi.org/10.1136/pgmj.2005.044156 PMid:17148700 PMCid:PMC2653923
Kokate C.K.; Purohit A.P. and Gokhale S.B. "Pharmacognosy" Nirali publication, 2009, 40th edition. Page no. 14.65-66. https://doi.org/10.1177/153567600901400201
Ulbricht C.; Abrams T.R.; Brigham A. and Ceurvels J. "An Evidence-Based Systematic Review of Rosemary (Rosmarinus officinalis) by the Natural Standard Research Collaboration"Journal of Dietary Supplements. 2010; 1-64.
Marin M.; Koko V.; Laušević D.S.; Marin P.D.; Rančić D. and Stevanovic Z.D. "Glandular trichomes on the leaves of Rosmarinus officinalis: Morphology, stereology and histochemistry". South African Journal of Botany. 2006; 72(3):378-382. https://doi.org/10.1016/j.sajb.2005.10.009
Koleilat M.; Raafat K.; El-LakanyA.andAboul-Ela M. "Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials". Pharmacogn J. 2017;9(4):452-474. https://doi.org/10.5530/pj.2017.4.75
Assami A.M.; Tomao V.; Ruiz K &Meklati B.Y. &Chema F. "Geographical Differentiation of Rosemary Based on GC/MS and Fast HPLC Analyses". Food Anal. Methods. 2013; 6(1):282-288. https://doi.org/10.1007/s12161-012-9430-6
Serrano E.; Palma J.; Tinocco T.; Venencio F. and Martins A. "Evaluation of the essential oils of rosemary (Rosmarinus officinalis L.) from different zones of "Alentejo" (Portugal). J. Ess. Oil Res. 2002; 14:87-92. https://doi.org/10.1080/10412905.2002.9699779
Hameed I.H.; and Mohammes G.J. "Phytochemistry, antioxidant, antibacterial activity and medicinal uses of aromatic (Medicinal plant Rosmarinus officinalis)" Aromatic and medicinal plants. 2017; 1-12. https://doi.org/10.5772/66605
Park S.E.; Kim S.; Sapkota K. and Kim S.J. "Neuroprotective effect of Rosmarinus officinalis extract on human dopaminergic cell line". SH-SY5Y. Cell. Molec. Neurobiol. 2001; 30(5):759-767. https://doi.org/10.1007/s10571-010-9502-3 PMid:20563702
Haloui M.; Louedec L.; Michel J. B. and Lyoussi B. "Experimental diuretic effects of Rosmarinus officinalis and Centaurium erythraea". J. Ethnopharm. 2007; 71: 465-472. https://doi.org/10.1016/S0378-8741(00)00184-7 PMid:10940584
Stamatelou KK, Francis ME, Jones CA, Nyberg LM, Curhan GC; Times trends in reported prevalence of kidney stone in the united state; 1976-1974; Kidney International,2003 May; 63(5);1817-1823. https://doi.org/10.1046/j.1523-1755.2003.00917.x PMid:12675858
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