Simultaneous Estimation of Pregabalin and Etoricoxib in Bulk and Pharmaceutical Dosage Form by Using RP-HPLC Method

  • Vislavath Hanumanth Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India
  • P Ravi Kumar Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India
  • Kaleru Sravya Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India
  • Yenumula Padmavathi Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India
  • Nayaka Raghavendra Babu Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India
  • Koppera Sowmya Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Abstract

A simple, accurate, precise method was developed for the simultaneous estimation of the Pregabalin and Etoricoxib in bulk and Pharmaceutical dosage form. Chromatogram was run through Ascentis (C18 150 x 4.8 mm, 2.8m). Mobile phase containing Buffer 0.01N Methanol: Na2HPO4 (0.1%OPA is added to adjust pH 5.4) taken in the ratio 70:30 was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 235nm. Retention time of Pregabalin and Etoricoxib were found to be 2.396 minutes and 2.968 minutes. %RSD of the Pregabalin and Etoricoxib were found to be 1.0 and 1.1 respectively. %Recovery was obtained as 99.82% and 99.99% for Pregabalin and Etoricoxib respectively. LOD, LOQ values obtained from regression equations of Pregabalin and Etoricoxib were 0.27, 0.81 and 0.18, 0.55 respectively. Regression equation of Pregabalin is y=3501.2x+839.82, and y=3696.3x+1383.5 for Etoricoxib. Retention time and run time was decreased, so the method was developed simple and economical that can be adopted in regular Quality control test.


Keywords: Pregabalin, Etoricoxib, RP-HPLC, Method validation

Keywords: Pregabalin, Etoricoxib, RP-HPLC, Method Vlidation

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Author Biographies

Vislavath Hanumanth, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

P Ravi Kumar, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Kaleru Sravya, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Yenumula Padmavathi, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Nayaka Raghavendra Babu, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Koppera Sowmya, Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

Department of Pharmaceutical Analysis, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad, Telangana 500028, India

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Hanumanth V, Ravi Kumar P, Sravya K, Padmavathi Y, Raghavendra Babu N, Sowmya K. Simultaneous Estimation of Pregabalin and Etoricoxib in Bulk and Pharmaceutical Dosage Form by Using RP-HPLC Method. JDDT [Internet]. 15Sep.2023 [cited 24Jun.2024];13(9):20-7. Available from: https://jddtonline.info/index.php/jddt/article/view/6200