Simultaneous Estimation of Pregabalin and Etoricoxib in Bulk and Pharmaceutical Dosage Form by Using RP-HPLC Method
Abstract
A simple, accurate, precise method was developed for the simultaneous estimation of the Pregabalin and Etoricoxib in bulk and Pharmaceutical dosage form. Chromatogram was run through Ascentis (C18 150 x 4.8 mm, 2.8m). Mobile phase containing Buffer 0.01N Methanol: Na2HPO4 (0.1%OPA is added to adjust pH 5.4) taken in the ratio 70:30 was pumped through column at a flow rate of 1ml/min. Temperature was maintained at 30°C. Optimized wavelength selected was 235nm. Retention time of Pregabalin and Etoricoxib were found to be 2.396 minutes and 2.968 minutes. %RSD of the Pregabalin and Etoricoxib were found to be 1.0 and 1.1 respectively. %Recovery was obtained as 99.82% and 99.99% for Pregabalin and Etoricoxib respectively. LOD, LOQ values obtained from regression equations of Pregabalin and Etoricoxib were 0.27, 0.81 and 0.18, 0.55 respectively. Regression equation of Pregabalin is y=3501.2x+839.82, and y=3696.3x+1383.5 for Etoricoxib. Retention time and run time was decreased, so the method was developed simple and economical that can be adopted in regular Quality control test.
Keywords: Pregabalin, Etoricoxib, RP-HPLC, Method validation
Keywords:
Pregabalin, Etoricoxib, RP-HPLC, Method Vlidation
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References
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21. Karanjkar MB, Development And Validation of RP-HPLC For Simultaneous Estimation of Pregabalin And Etoricoxib In Pharmaceutical Tablet Dosage Form, IJPSR 2021; 13(4):872-879.
22. Swarna Pushpa MS, RP-HPLC Quantifiable Technique Development For Evaluating Pregabalin And Etoricoxib Combination In Tablet And Bulk Kinds, International Journal of Applied Pharmaceutics 2021; 13(6): 152-156. https://doi.org/10.22159/ijap.2021v13i6.42759
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24. Upeksha B, TaraiDK, Development and Validation for Simultaneous Estimation of Etoricoxib and Pregabalin in Bulk and Tablet Dosage Form by RP-HPLC, International Journal of All Research Education & Scientific Method 2021, 9(5).
25. Prakash M, Method Development And Validation Of Pregabalin And Etoricoxib In Bulk And Pharmaceutical Dosage Form By RP-HPLC Method, IAJPS 2022; 09(01):231-237.
26. Srinivasu T, Validated RP-HPLC Method for the Assay of Etoricoxib (A Non-Steroidal Anti-Inflammatory Drug) in Pharmaceutical Dosage Forms, E-Journal of Chemistry 2012; 9(2):832-838. https://doi.org/10.1155/2012/264567
2. Lindholm J, Development and Validation of HPLC Method for Analytical and Preparation Purpose. Acta Universitatis Upsaliensis, 2004:13-14.
3. Rashmin, An introduction to analytical Method Development for Pharmaceutical formulations. Indoglobal Journal of Pharmaceutical Sciences, 2012; 2(2):191-196.
4. Malvia R, Bansal V, Pal OP and Sharma PK, A Review of High Performance Liquid Chromatography. Journal of Global Pharma technology 2010.
5. Douglas A Skoog, James Holler F, Timothy A, Niemen, Principles of Instrumental Analysis :725-760.
6. Dr. S Ravi Shankar, Text book of Pharmaceutical analysis, Fourth edition:13.1-13.2.
7. David G Watson, Pharmaceutical Analysis, A text book for Pharmacy students and Pharmaceutical Chemists. Harcourt Publishers Limited; 2nd Edition:221-232.
8. Remingtonn's The Sciences and Practise of Pharmacy, 20th Edition 2000.
9. Connors KA, A Textbook of Pharmaceutical Analysis, Wiley intersciences Inc; Delhi, 3rd Edition, 1994:373-421.
10. David G Watson Pharmaceutical Analysis, A text book for pharmacy students and Pharmaceutical Chemists. Harcourt Publishers Limited; 2nd Edition:267-311.
11. Nasal A, Siluk D, and Kaliszan R, Chromatographic Retention Parameters in Medicinal Chemistry and Pharmacology, Pubmed, 2003; 10(5):381-426. https://doi.org/10.2174/0929867033368268 PMid:12570698
12. Kumar A, Kishore L, Kaur N, Nair A, Method Development and Validation for Pharmaceutical Analysis. International Pharmaceutical Sciences, 2012; 2(3). https://doi.org/10.4103/2229-5186.94303
13. Kaushal C, Srivatsava B, A Process of Method Development: A Chromatographic Approach. J Chem Pharm Res, 2010; 2(2):519-545.
14. Gupta V, Jain ADK, Gill, Kapil NS, Development and Validation of HPLC method. International Research Journal of Pharmaeutical and Applied Sciences, 2012; 2(4).
15. Hokanson GC, A life cycle approach to the validation of analytical methods during Pharmaceutical Product Development. Part 1: The Initial Validation Process. Pharm Tech 1994:92-100.
16. Green JM, A Practicle guide to analytical method validation, Anal Chem 1996:305A-309A. https://doi.org/10.1021/ac961912f
17. ICH, Validation of analytical procedures: Text and Methodology. International Conference on Harmonization, IFPMA , Geneva , 1996.
18. IUPAC, Compendium of Chemical Terminology, 2nd Edition. (The Gold Book). PAC69, 1137 1997.
19. Indian Pharmacopoeia, Indian Pharmacopoeial Commission, Controller of Publication, Government of India, Ministry of health and Family Welfare, Ghaziabad, India, 2010:1657-1658.
20. British Pharmacopoeia, The British Pharmacopoeial Commission, the stationary office, UK, London, 2011:1408-1409.
21. Karanjkar MB, Development And Validation of RP-HPLC For Simultaneous Estimation of Pregabalin And Etoricoxib In Pharmaceutical Tablet Dosage Form, IJPSR 2021; 13(4):872-879.
22. Swarna Pushpa MS, RP-HPLC Quantifiable Technique Development For Evaluating Pregabalin And Etoricoxib Combination In Tablet And Bulk Kinds, International Journal of Applied Pharmaceutics 2021; 13(6): 152-156. https://doi.org/10.22159/ijap.2021v13i6.42759
23. Chaudhary A, Singh BK, Simultaneous Estimation of Pregabalin and Etoricoxib using Novel HPLC Method: An Application in Quantitative Analysis of Pharmaceutical Dosage Forms, Indian Journal of Pharmaceutical Education and Research, 2021; 55(4S). https://doi.org/10.5530/ijper.55.3s.191
24. Upeksha B, TaraiDK, Development and Validation for Simultaneous Estimation of Etoricoxib and Pregabalin in Bulk and Tablet Dosage Form by RP-HPLC, International Journal of All Research Education & Scientific Method 2021, 9(5).
25. Prakash M, Method Development And Validation Of Pregabalin And Etoricoxib In Bulk And Pharmaceutical Dosage Form By RP-HPLC Method, IAJPS 2022; 09(01):231-237.
26. Srinivasu T, Validated RP-HPLC Method for the Assay of Etoricoxib (A Non-Steroidal Anti-Inflammatory Drug) in Pharmaceutical Dosage Forms, E-Journal of Chemistry 2012; 9(2):832-838. https://doi.org/10.1155/2012/264567
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Hanumanth V, Ravi Kumar P, Sravya K, Padmavathi Y, Raghavendra Babu N, Sowmya K. Simultaneous Estimation of Pregabalin and Etoricoxib in Bulk and Pharmaceutical Dosage Form by Using RP-HPLC Method. JDDT [Internet]. 15Sep.2023 [cited 2Oct.2023];13(9):20-7. Available from: https://jddtonline.info/index.php/jddt/article/view/6200
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