Comparative UV Spectroscopic Method Analysis and Validation for Estimation of Rifaximin in Pharmaceutical Preparation

  • Aney Joice Assistant Professor Department of Pharmaceutics, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India.
  • Farheen Mohammed Zubair Sange Postgraduate Student M. Pharm Department of Pharmaceutical Quality Assurance, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India

Abstract

In the present research work the absorption characteristics of rifaximin was studied and validated in different solvents in visible region for its quantitative estimation in bulk and dosage forms by UV spectrophotometric method. The solution of Rifaximin were prepared in 0.1N HCL (pH 1.2), Phosphate buffer (pH 6.8) & Phosphate buffer (pH 7.4) and showed maximum absorption at wavelength 439 nm, 440 nm & 433 nm in respective solvents. The drug obeyed Beer–Lambert’s law in the concentration range of 2, 4, 6, 8, 10 µg/mL with coefficient of correlation (r2) of 0.998. The estimation method was validated as per the ICH guidelines. Statistical analysis proved that the methods are repeatable and specific for the estimation of the said drug. These methods can be adopted in routine analysis of Rifaximin in bulk or tablet dosage form.


Keywords: Rifaximin, 0.1N HCL, phosphate buffer, UV spectrophotometer.

Keywords: Rifaximin, 0.1N HCL, phosphate buffer, UV spectrophotometer

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Author Biographies

Aney Joice, Assistant Professor Department of Pharmaceutics, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India.

Assistant Professor Department of Pharmaceutics, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India.

Farheen Mohammed Zubair Sange, Postgraduate Student M. Pharm Department of Pharmaceutical Quality Assurance, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India

Postgraduate Student M. Pharm Department of Pharmaceutical Quality Assurance, M.C.E Society’s Allana College of Pharmacy, Pune-411001, Maharashtra, India

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Joice A, Sange FMZ. Comparative UV Spectroscopic Method Analysis and Validation for Estimation of Rifaximin in Pharmaceutical Preparation. JDDT [Internet]. 15Sep.2023 [cited 24Jun.2024];13(9):33-0. Available from: https://jddtonline.info/index.php/jddt/article/view/6193