In Vitro Antioxidant and Ex Vivo Anticataract Activity of Hydroalcoholic Extract of Cordia obliqua Willd
Abstract
Cordia obliqua has a long history of use in the treatment of cataract and other eye-related problems in Indian traditional medicine. High oxidative stress is one of the major underlying causes of cataract which results in the precipitation of natural protein present in the lenses with aging. This research has been carried out to determine the anti-cataract activity of Cordia obliqua by performing various antioxidant techniques such as 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide, ABTS radical cation and Ferric reducing antioxidant potential studies in oxidative stress–induced ex vivo cataract model. Results of the study conducted in the hydroalcoholic extract of leaves of Cordia obliqua revealed the presence of various phytoconstituents such as alkaloids, phenols, flavonoids, etc. Total phenol and total flavonoid content was found to be 53 ± 3.21and 26.12 ± 2.54% respectively, which revealed that the plant contains a good amount of these compounds and hence possesses good antioxidant activity. Furthermore, IC50 values obtained from all the methods gave strong evidence regarding the antioxidant potential of this plant. Anti-cataract activity was also investigated using goat eye lenses and promising results were obtained which speak voluminously about its anti-cataract potential and support its well-prescribed use. Results obtained with this study clearly supported the significant antioxidant potential and anticataract activity of this plant. Further, this plant demands great attention for the development of suitable novel dosage forms for the effective treatment of cataract.
Keywords: Cordia obliqua, cataract, High oxidative stress
Keywords:
Cordia obliqua, cataract, High oxidative stressDOI
https://doi.org/10.22270/jddt.v13i1.5727References
Congdon N, Vingerling JR, Klein BE, West S, Friedman DS, Kempen J, et al. Prevalence of cataract and pseudophakia/aphakia among adults in the United States. Arch of Ophthalmol 2004; 122:487-494. https://doi.org/10.1001/archopht.122.4.487
Bhat K. Scavenging of peroxide and related oxidants in human brunecent cataracts. In: Gupta SK, editor. Ocular pharmacology: recent advances. New Delhi, India: Indian Ocular Pharmacological Society; 1991. p. 32-8.
Wolff SP. Diabetes mellitus and free radicals. Br Med Bull 1993; 49:642-652. https://doi.org/10.1093/oxfordjournals.bmb.a072637
Klivenyi P, Andreassen OA, Aerrante RJ, Dedeoglu A, Mueller G, Lancelot E, Bogdanov M, Andersen JK, Jiang D, Beal MF. Mice deficient in cellular glutathione peroxidase show increased vulnerability to malonate, 3-nitroproprionicacid, and 1-methyl- 4-phenyl-1,2,5,6-etrahydropyridine. J Neurosci. 2000; 20:1-7. https://doi.org/10.1523/JNEUROSCI.20-01-00001.2000
Varma SD, Hedge KR. Effect of alpha-ketoglutarate against selenite cataract formation. Exp Eye Res 2004; 79:913-918. https://doi.org/10.1016/j.exer.2004.06.012
Gupta SK, Joshi S, Velpandian T, Awor L, Prakash J. An update on pharmacological prospectives for prevention and development of cataract. Indian J Pharmacol 1997; 29:3-10. 7. Bunce GE, Kinoshita J, Horwitz J. Nutritional factors in cataract. Ann Rev Nutr 1990; 10:233-254. https://doi.org/10.1146/annurev.nu.10.070190.001313
Thirupathi K, Kumar SS, Raju VS, Ravikumar B, Krishna DR, Krishna MG. A review of medicinal plants of the genus Cordia: Their chemistry and Pharmacological uses. Journal of Natural Remedies. 2008; 8(l):1-10
Khare CP. Indian Medicinal Plants: An Illustrated Dictionary. Springer-Verlag Berlin/Heidelberg New York USA. 2007 page 173-4. https://doi.org/10.1007/978-0-387-70638-2
Dutta R, Sharma MK, Jha M. Pharmacological evaluation of antiasthmatic activity of fumaria officinalis extracts. Plant Archives. 2020; 20(2):4308-15.
Rani D, Kharkwal H, Jha M, Rai N. Assessment of the total flavonoid, phenol, alkaloid content and sun protection factor in grewia abutilifolia leaf extract. Journal of Pharmaceutical Research International.2021; 33(49A):42-51. https://doi.org/10.9734/jpri/2021/v33i49A33300
Joshi S, Parkhe G, Aqueel N, Dixit N, Jain DK. Estimation of total phenolic, total flavonoids and total protein content of hydroalcoholic extract of Anacyclus pyrethrum. Pharmacologyonline. 2019; 1:27-33.
Martinello F, Soares SM, Franco JJ, Santos AC, Sugohara A, Garcia SB, Curti C, Uyemura SA. Hypolipemic and antioxidant activities from Tamarindusindica L. pulp fruit extract in hypercholesterolemic hamsters. Food Chem Toxicol. 2006; 44:810-818. https://doi.org/10.1016/j.fct.2005.10.011
Dutta R, Sharma MK, Khan A, Jha M. Phytochemical and in vitro antioxidant assay of Fumaria officinalis leaf extract. Journal of Advanced Scientific Research. 2020; 11(03):176-82.
Gadekar S, Goyal S, Khan A, Jha M. Chemopreventive action of sphaeranthus indicus on dmba-induced skin papillomagenesis in mice. Journal of Advanced Scientific Research. 2020; 11(3):161-167.
Chandorkar AG, Albal MV, Bulakh PM. Muley MP. Lens Organ Culture. Indian J Opthalmol 1981; 29:151-2.
Ellman GL. Tissue Sulfhydryl groups. Arch BiochemBiophys. 1959; 82: 70-77. https://doi.org/10.1016/0003-9861(59)90090-6
Goth L. A simple method for determination of serum catalase activity, and revision of reference range. ClinChimActa 1991; 196:143- 52. https://doi.org/10.1016/0009-8981(91)90067-M
Gupta SK, Joshi S. Role of Naproxen as antioxidant is selenite cataract. Ophthalmic Res 1994; 26:226-231. https://doi.org/10.1159/000267478
Harding JJ, Rixon KC. Carbamylation of lens proteins: A possible factor in cataractogenesis in some tropical countries. Exp eye res 1980; 31:567-71. https://doi.org/10.1016/S0014-4835(80)80015-7
Singleton V, Rossi JJ. Colorimetry of total phenolics with phosphor molybdic phosphotungstic acid reagents. Am J Enol Viticult. 1965; 16:144-158.
Kinoshita JH, Merola LU, Dikmak E. The accumulation of dulcitol and water in rabbit lens incubated with galactose. Biochem Biophys Acta 1962; 62:176-78. https://doi.org/10.1016/0006-3002(62)90508-5
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