Organogenesis from Leaf-derived Calli of S. potatorum L. f.: A Vulnerable Medicinal Tree Species

  • VEERAN VIJAYA KUMAR P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India
  • RAJAKUMARAN PATHMAVATHI SARALLA P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India
  • PALANISAMY - KANNAN P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

Abstract

An efficient protocol for in vitro regeneration of Strychnos potatorum L. f. through indirect organogenesis was developed using mature leaf disc explants. The explants were selected from twenty years old tree. Cultured on Murashige and Skoog’s medium supplemented with 2,4-dichloro phenoxy acetic acid (2,4-D) was used for the induction of organogenic calli. A pale yellow coloured friable callus developed at 2 mg/L-1 2,4-D where showed higher morphogenic potential. Further de-novo shoots formation and multiplication in optimal concentrations of cytokinins with different combinations of auxins.  An average mean shoots number (14.2±0.42) and shoots height (3.5±0.27cm) was obtained from piece of calli in 6-benzylaminopurine (BAP) 1 mg/L-1 with 0.5 mg/L-1 α-naphthalene acetic acid (NAA). BAP in combinations with NAA was found to be superior to shoot development than other combination used. The well developed shoots were transferred to root induction medium for effective rooting. The rooting medium consisted of in half-strength MS medium augmented with 0.5 mg/L-1 NAA was used. The maximal mean number of roots per shoots 6.5±0.53 was recorded in 4 weeks. The rooted plantlets were removed from the culture tubes, gently washed with sterile double distilled water to remove agar adhering on the plant surface. The regenerated plants were transferred to plastic cups filled with a potting mixture containing sterile sand and soilrite (1:1 ratio) and allowed to grow in the greenhouse. The slowly acclimatized plants were further transferred to substrate containing red soil and farmyard manure (1:1) for effective hardening. 75% survival rate was recorded. This protocol can be useful for the ex-situ conservation and rehabilitation of this vulnerable medicinal tree S. potatorum.


Keywords: Indirect organogenesis, friable callus, shoot development, 6-benzylaminopurine, α-naphthalene acetic acid

Keywords: Indirect organogenesis, friable callus, shoot development, 6-benzylaminopurine, α-naphthalene acetic acid

Downloads

Download data is not yet available.

Author Biographies

VEERAN VIJAYA KUMAR, P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

RAJAKUMARAN PATHMAVATHI SARALLA, P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

PALANISAMY - KANNAN, P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

P.G. & Research Department of Botany, The Madura College (Autonomous), Madurai - 625 011, Tamil Nadu, India

References

1. Sanmugapriya E, Venkataraman S. Anti-ulcerogenic potential of Strychnos potatorum Linn. seeds on aspirin plus pyloric ligation induced ulcers in experimental rats. Phytomedicine. 2007; 14:360-5. https://doi.org/10.1016/j.phymed.2006.12.025
2. Mishra SB, Verma A, Vijayakumar M. Preclinical evaluation of anti-hyperglycemic and antioxidant action of Nirmali (Strychnos potatorum) seeds in streptozotocin-nicotinamide- induced diabetic wistar rats: a histopathological investigation. Biomark. Genom. Med. 2013; 5:157-163. https://doi.org/10.1016/j.bgm.2013.07.010
3. Tripathi PN, Chaudhuri N, Bokil SD. Nirmali seed a naturally occurring coagulant. Indian J Environ Health. 1976; 18:72-81.
4. Sarawgi G, Kamra A, Suri N, Kaur A, Sarethy IP. Effect of Strychnos potatorum Linn. Seed extracts on water samples from different sources and with diverse properties. Asian J Water Environ. Pollut. 2009; 6:13-17.
5. Kirtikar KR, Basu BD. Illustrated Indian Medicinal Plants.Edited by: Mhaskar K S, Blatter E, Cains J F. Sir Satguru's Publications. 2000. pp. 2271.
6. Bisset NG, The Asian species of Strychnos part III: the ethnobotany. Lloydia. 1974; 37:62-107.
7. Kagithoju S, Godishala V, Kairamkonda M, Kurra H, Nanna RS. Recent advances in elucidating the biological and chemical properties of Strychnos potatorum Linn. f.-a review. Int. J Pharm. Bio. Sci. 2012; 3:291-303.
8. Kagithoju S, Godishala V, Kairamkonda M, Nanna RS. Embryo culture is an efficient way to conserve a medicinally important endangered forest tree species Strychnos potatorum. J For Res. 2013; 24:279-283. https://doi.org/10.1007/s11676-013-0350-0
9. Murashige T, Skoog F, A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 1962; 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
10. Singh CK, Raj SR, Patil VR, Jaiswal PS, Subhash N. Plant regeneration from leaf explants of mature sandalwood (Santalum album L.) trees under in vitro conditions. In Vitro Cell. Dev. Biol. 2013; 49:216-222. https://doi.org/10.1007/s11627-013-9495-y
11. Omamor IB, Asemota AO, Eke CR, Ezia EI. Fungal contaminants of the oil palm tissue culture in Nigerian Institute for Oil Palm Research (NIFOR). Afr. J. Agric. Res. 2007; 2:534-537.
12. Isah T. De-novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex. Sm. Isah Biol Res. 2019; 52:3. https://doi.org/10.1186/s40659-019-0211-1
13. Pan Z, Zhu S, Guan R, Deng X. Identification of 2, 4-D-responsive proteins in embryogenic callus of valencia sweet orange (Citrus sinensis Osbeck) following osmotic stress. Plant Cell Tiss Organ Cult. 2010; 103:145-153. https://doi.org/10.1007/s11240-010-9762-0
14. Hesami M, Daneshvar MH, Najafabadi YM, Alizadeh M. Effect of plant growth regulators on indirect shoot organogenesis of Ficus religiosa through seedling derived petiole segments. J. Genet. Eng. Biotechnol. 2018; 16:175-180. https://doi.org/10.1016/j.jgeb.2017.11.001
15. Qing ZY, Jie ZM, Deng Z, Jie ZJ, Jian LJ, Yang CX. In vitro plant regeneration of Zenia insignis Chun. Open Life Sci. 2018; 13:34-41. https://doi.org/10.1515/biol-2018-0005
16. Koetle MJ, Finnie JF, Balázs E, Staden JV. A review on factors affecting the Agrobacterium-mediated genetic transformation in ornamental monocotyledonous geophytes. S. Afr. J. Bot. 2015; 98:37-44. https://doi.org/10.1016/j.sajb.2015.02.001
17. Li J, Zhang D, Ouyang K, Chen X. High frequency plant regeneration from leaf culture of Neolamarckia cadamba. Plant Biotechnology. 2019; 36:13-19. https://doi.org/10.5511/plantbiotechnology.18.1119a
18. Venkatachalam P, Kalaiarasi K, Sreeramanan S. Influence of plant growth regulators (PGRs) and various additives on in vitro plant propagation of Bambusa arundinacea (Retz.) Wild: A recalcitrant bamboo species. J. Genet. Eng. Biotechnol. 2015; 13:193-200. https://doi.org/10.1016/j.jgeb.2015.09.006
19. Das P, Tanti B, Borthakur SK, In vitro callus induction and indirect organogenesis of Brucea mollis Wall. ex Kurz - A potential medicinal plant of Northeast India. J. Genet. Eng. Biotechnol. 2018; 119:203-211. https://doi.org/10.1016/j.sajb.2018.09.012
20. Patricia D, Stephen B, John A. Shoot organogenesis from leaf discs of the African ginger (Mondia whitei (Hook.f.) Skeels), an endangered medicinal plant. In Vitro Cell.Dev.Biol. 2021; 57:1-6. https://doi.org/10.1007/s11627-020-10146-0
21. Huang H, Wei Y, Zhai Y, Ouyang K, Chen X, Bai L. High frequency regeneration of plants via callus-mediated organogenesis from cotyledon and hypocotyl cultures in a multipurpose tropical tree (Neolamarkia cadamba). Scientific Reports. 2020; 10:4558. https://doi.org/10.1038/s41598-020-61612-z
22. Manokari M, Priyadharshini S, Shekhawat M S, Micropropagation of sea grape (Coccoloba uvifera (L.) L.). S. Afr. J. Bot. 2021; 250-258. https://doi.org/10.1016/j.sajb.2020.04.028
Statistics
171 Views | 17 Downloads
How to Cite
1.
VIJAYA KUMAR V, SARALLA R, KANNAN P. Organogenesis from Leaf-derived Calli of S. potatorum L. f.: A Vulnerable Medicinal Tree Species. JDDT [Internet]. 15Jun.2022 [cited 1Jul.2022];12(3-S):169-74. Available from: https://jddtonline.info/index.php/jddt/article/view/5528