Mesalazine based topical hydrogel formulation enhances anti-oxidant and cytokine activity in wounded STZ-induced mice

  • Shreshtha Gaur Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA https://orcid.org/0000-0002-9267-9684
  • Sonu Singhal Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA
  • Sonal Gaur Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA
  • Rakesh Mishra Getwell Polyclinic and Hospital, Jaipur, JLN Marg, Rajasthan, 302004, India https://orcid.org/0000-0001-7352-8293
  • Ishan Pandey Department of Pathology, Motilal Nehru Medical College, Prayagraj- 211001, Uttar Pradesh. INDIA
  • Surabhi Bajpai Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA https://orcid.org/0000-0002-2093-7437

Abstract

Background: The present study is targeted to elucidate the wound healing potential of mesalazine in STZ induced diabetic mice by comparing various antioxidant, pro-inflammatory cytokine levels and other wound healing parameters at 3,7 and 14th day.


Methods: Full thickness excisional wounds of 6mm size were created on the dorsal side of STZ induced mice and topical treatment of mesalazine based different hydrogels was applied for 14 days. Wound tissues were excised on day 3, 7 and 14 for various wound healing parameters.


Results: Delayed in wound healing was observed in diabetic group which eventually got accelerated after application of ethosome based mesalazine loaded hydrogel designated as D5 in study. Significant wound contraction rate was observed in D5 group and tissue hydroxyproline and tensile strength was also elevated after treatment with mesalazine loaded ethosomal hydrogel. The level of ROS was found to be significantly decreased in SOD, CAT and GSH experiment. LPO level were found to be elevated in D5 group. Finding of levels of pro-inflammatory cytokine suggested significant decrease and showed significant elevation in vascular endothelial growth factor (VEGF). 


Conclusion: D5 group of ethosome based mesalazine loaded hydrogel showed promising results in controlling all the factors to their normal range and was effective in accelerating wound 51healing in diabetic mice. 


Keywords: Diabetic wounds; hydrogels; antioxidants; cytokines; VEGF; Mesalazine

Keywords: Diabetic wounds, hydrogels, antioxidants, cytokines, VEGF, Mesalazine

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Author Biographies

Shreshtha Gaur, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Sonu Singhal, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Sonal Gaur, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Rakesh Mishra, Getwell Polyclinic and Hospital, Jaipur, JLN Marg, Rajasthan, 302004, India

Getwell Polyclinic and Hospital, Jaipur, JLN Marg, Rajasthan, 302004, India

Ishan Pandey, Department of Pathology, Motilal Nehru Medical College, Prayagraj- 211001, Uttar Pradesh. INDIA

Department of Pathology, Motilal Nehru Medical College, Prayagraj- 211001, Uttar Pradesh. INDIA

Surabhi Bajpai, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali-304022, Rajasthan, INDIA

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1.
Gaur S, Singhal S, Gaur S, Mishra R, Pandey I, Bajpai S. Mesalazine based topical hydrogel formulation enhances anti-oxidant and cytokine activity in wounded STZ-induced mice. JDDT [Internet]. 15Mar.2022 [cited 25Sep.2022];12(2):51-7. Available from: https://jddtonline.info/index.php/jddt/article/view/5384