ANTIMICROBIAL INVESTIGATION OF PIPER NIGRUM L. AGAINST SALMONELLA TYPHI

  • Vishal Kumar Deshwal Department of microbiology, Doon (P.G.) Paramedical College, Dehardun-248001 (India)

Abstract

Aim of this study was to evaluate the antibacterial activity of different extracts of Piper nigrum L. against Salmonella typhi. Widal (serological) test positive patients were selected. Salmonella strains were isolated from blood of infected persons. 10 samples were analyzed for present study. Strains were cultured on blood agar medium and Bismuth Sulfite Agar medium. Pure isolated strains were characterized on the basis of Bergey’s Manual of determinative bacteriology. Three extracts such as aqueous, ethanolic and chloroform extracts of Piper nigrum seeds were prepared. 4 different concentrations such as 15, 20, 25, 30 mg/ml of them were made. Antimicrobial activity was determined by Agar well diffusion method. Results showed that 30 mg/ml of aqueous, ethanol and chloroform extract of Piper nigrum gave 23.4±0.2, 21.8±0.4, 20.4±0.1 mm of inhibition zones, respectively. Aqueous extract was observed to be best among all extract of Piper nigrum.

Keywords: Piper nigrum, Salmonella typhi

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1.
Deshwal V. ANTIMICROBIAL INVESTIGATION OF PIPER NIGRUM L. AGAINST SALMONELLA TYPHI. JDDT [Internet]. 13May2013 [cited 14Apr.2024];3(3):100-3. Available from: https://jddtonline.info/index.php/jddt/article/view/486