Study on correlation of Antioxidant activities with presence of phenolic and Flavanoid contents in Emblica officinalis and Terminalia chebula
Background: Reactive oxygen species have been known to cause cellular damages that have been implicated to be a causal of major diseases; therefore natural antioxidants have shown a significant impact on human robustness. The present study was carried out to appraise the anti-oxidant activities (In- vitro) and their correlation with presence of Flavanoid and Phenolic content in fruits of Terminalia chebula and Emblica officinalis fruit extracts which is common in herbal Kitchen of India.
Methods: The 70% extracts of fruits from Terminalia chebula and Emblica officialis were applied for the study of Anti-oxidant activity. Scavenging radical ability of extracts of these extracts were judged by radical like DPPH.
Results: The capability of the extracts of Fruits in exhibiting Antioxidative properties follow the sequence of Terminalia chebula > Emblica officinalis. Since the antioxidant activities were studied in comparison with the standards of Flavanoid, Phenolic and Ascorbic acid. The Flavanoid and Phenolic quantity / amount along with subsequent dilution of Ascorbic acid as in case of DPPH radical assay were assessed as 127.60 ± 0.001 mg/ml, 133.00 ± 0.003 mg/ml for Phenolic Content as Gallic acid equivalent per 100 mg of the Fruit extract.
Keywords: Antioxidant, Phenol, Flavanoid, Emblica, Terminalia.
2. Maxwell SR, Prospects for the use of antioxidant therapies, Drugs, 1995; 49:345–361.
3. Niki E, Shimaski H, Mino M, Antioxidant-free radical and biological defense, 1994; 3–16.
4. Finkel T, Holbrook NJ, Oxidants, oxidative stress and the biology of ageing, Nature, 2000; 408:239–247.
5. Chopra RN, Nayar SL, Chopra IC, Glossary of Indian Medicinal Plants. CSIR, New Delhi, India, 1956; 106:241- 242.
6. Lee HS, Won NH, Kim KH, Lee H, Jun W, Lee KW, Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro, Biology and Pharmaceutical Bulletin, 2005; 28:1639–1644.
7. Aslokar L, Kakkar KK, Chakre OJ, Supplement to Glossary of Indian Medicinal Plants with Active Principles. Directorate CSIR, New Delhi, India, 1992; 291–293.
8. Rajarama Rao MR, Siddiqui HH, Pharmacological studies on Emblica officinalis, Indian Experimental Biology, 1964; 2:29–31.
9. Halliwell B, Gutteridge JMC, Free Radicals in Biology and Medicine, Oxford, England, 1985; 279–315.
10. Roy AK, Dhir H, Talukdar G, Phyllanthus emblica fruit extract and ascorbic acid modify hepatotoxic and renotoxic effects of metals in mice, International Journal of Pharmacology, 1991; 29:117–126.
11. Duarte MCT, Antimicrobial Activity of Medicinal and Aromatic Plants Used in Brazil, MultiCiência, 2006; 7.
12. Mohammed Ali, Text Book of Pharmacognosy, Delhi, India, 1998; 374.
13. Ames BN, Shigenaga MK, and Hagen TM, Oxidants, antioxidants, and the degenerative diseases of aging in the Proceedings of the National Academy of Sciences of the United States of America, 1993; 90(17):7915–7922.
14. Block G, Patterson B, and Subar A, Fruit, vegetables, and cancer prevention: a review of the epidemiological evidence, Nutrition and Cancer, 1992; 18(1):1–29.
15. Vinson JA, Dabbagh JYA, Serry MM, Cai S, Plant polyphenols exhibit lipoprotein-bound antioxidant activity using an in vitro oxidation model for heart disease, Journal of Agricultural and Food Chemistry,1995; 43(11):2798–2799.
16. Paganga G, Miller M, Rice-Evans CA, The polyphenolic content of fruit and vegetables and their antioxidant activities. What does a serving constitute, Free Radical Research, 1999; 30(2):153–162.
17. Kähkönen MP, Hopia AI, Vuorela HJ, Antioxidant activity of plant extracts containing phenolic compounds, Journal of Agricultural and Food Chemistry, 1999; 47(10):3954–3962.
18. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB, The relative antioxidant activities of plant-derived polyphenolic flavonoids, Free Radical Research, 1995; 22 (4):375–383.
19. Sugihar T, Arakawa MO, Furuno K, Anti- and pro-oxidative effects of flavonoids on metal-induced lipid hydroperoxide-dependent lipid peroxidation in cultured hepatocytes loaded with α-linolenic acid, Free Radical Biology and Medicine,1999; 27(11):1313–1323, 1999.
20. Rice-Evans CA, Miller NJ, and Paganga G, Structure-antioxidant activity relationships of flavonoids and phenolic acids, Free Radical Biology and Medicine, 1996; 20 (7):933–956.
21. Ramarathnam N, Ochi H, Takeuchi M, Antioxidant defense system in vegetable extracts in Natural Antioxidants, Chemistry, Health Effects and Applications, 1997: 76–87.
22. Tapiero H, Tew KD, Ba GN, Mathé G, Polyphenols: do they play a role in the prevention of human pathologies, Biomedicine and Pharmacotherapy, 2002; 56(4):200–207.
23. Sgherri C, Cecconami S, Pinzino C, Navari-Izzo F, and Izzo R, Levels of antioxidants and nutraceuticals in basil grown in hydroponics and soil, Food Chemistry, 2010; 123(2):416–422
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