Formulation and Cytotoxic Characterization of Trigonella foenum Loaded Polymeric Nanoparticles
Abstract
The aim of present study was to formulate herbal Trigonella Foenum (TF) loaded nanoparticles using hydrophilic polymer at different concentration and optimizing the nanoparticle formulation. Herbal drug having high molecular size or poor water solubility, suffers from less assimilation and poor bio accessibility and hence loading of TF in nanoparticles improves the systematic drug delivery approaches. The formulation containing 3 % of polymer PVP showed optimum result with encapsulation efficiency of 77.73±0.28 and loading efficiency of 16.98±0.7. Simultaneously showing high stability with zeta potential of -23.7±0.98 mV and PDI of 215. The mean average particle size of nanoparticles was found to be 432.1 ± 16.01. FT-IR studies suggested compatibility of polymer and drug when observed with major peaks and functional groups. The in-vitro SRB cytotoxicity assay showed a maximum inhibition of cell viability of 71±2.3 % and IC 50 value of 100±10.2 ug/ml.
Keywords: Trigonella Foenum, poor bioavailability, polymeric nanoparticle , cytotoxicity assay
Keywords:
Trigonella Foenum, poor bioavailability, polymeric nanoparticle, cytotoxicity assayDOI
https://doi.org/10.22270/jddt.v10i4-s.4332References
Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018; 9:1050-1074. doi: 10.3762/bjnano.9.98.
Wicki A, Witzigmann D, Balasubramanian V, Huwyler J. Nanomedicine in cancer therapy: challenges, opportunities, and clinical applications. J Control Release. 2015; 200:138–157. doi: 10.1016/j.jconrel.2014.12.030.
Welden, G., Prince, J., Plant Compounds Continue to Challenge Science, The American Institute for Cancer Research, www.aicr.org2006.
Ajazuddin, Saraf S. Applications of novel drug delivery system for herbal formulations. Fitoterapia. 2010Oct; 81(7):680-9. doi: 10.1016/j.fitote.2010.05.001.
Bailey MM, Berkland CJ. Nanoparticle formulations in pulmonary drug delivery. Med Res Rev. 2009; 29:196212.
Bahmani M, Shirzad H, Mirhosseini M, Mesripour A, Rafieian-Kopaei M, A review on ethnobotanical and therapeutic uses of fenugreek (Trigonella foenum-graceum L), J. Evid. Based Complement. Altern. Med. 21 (2016) 53– 62, doi:http://dx.doi.org/10.1177/2156587215583405.
Hua S, Y. Li, L. Su, X. Liu, Diosgenin ameliorates gestational diabetes through inhibition of sterol regulatory element-binding protein-1, Biomed. Pharmacother. (2016), doi:http://dx.doi.org/10.1016/j.biopha.2016.10.049
Chaudhary SA, Chaudhary PS, Syed BA, Misra R, Bagali PG, Vitalini S, Iriti M. Validation of a method for diosgenin extraction from fenugreek (Trigonella foenum-graecum L.). Acta Sci Pol Technol Aliment. 2018 Oct-Dec; 17(4):377-385. doi: 10.17306/J.AFS.0606.
Kanyas S, Aydın D, Kizilel R, Demirel AL, Kizilel S. Nanoparticle and gelation stabilized functional composites of an ionic salt in a hydrophobic polymer matrix. PLoS One. 2014; 9(2):e88125. Published 2014 Feb 6. doi:10.1371/journal.pone.0088125
Neyshaburinezhad N, Kalalinia F, Hashemi M. Encapsulation of crocetin into poly (lactic-co-glycolic acid) nanoparticles overcomes drug resistance in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS). Mol Biol Rep. 2019 Dec; 46(6):6525-6532. doi: 10.1007/s11033-019-05098-7.
Cahalane C, Bonezzi J, Shelestak J, Clements R, Boika A, Yun YH, Shriver LP. Targeted delivery of anti-inflammatory and imaging agents to microglial cells with polymeric nanoparticles. Mol Pharm. 2020 Mar 26;. doi: 10.1021/acs.molpharmaceut.9b00489.
Dao UPN, Nguyen QD, Nguyen TT. Regulation of Lipid Membrane Partitioning of Tamoxifen by Ionic Strength and Cholesterol. Pharm Res. 2020 Feb 10; 37(3):53. doi: 10.1007/s11095-020-2771-8.
Orellana EA, Kasinski AL. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5; 6(21). doi: 10.21769/BioProtoc.1984.
Vijayanand, P., Jyothi, V., Aditya, N., & Mounika, A. Development and Characterization of Solid Lipid Nanoparticles Containing Herbal Extract: In Vivo Antidepressant Activity. Journal of Drug Delivery, 2018; 1–7. doi:10.1155/2018/2908626
Haque ST, Karim ME, Abidin SAZ, Othman I, Holl MMB, Chowdhury EH. Fe/Mg-Modified Carbonate Apatite with Uniform Particle Size and Unique Transport Protein-Related Protein Corona Efficiently Delivers Doxorubicin into Breast Cancer Cells. Nanomaterials (Basel). 2020 Apr 27; 10(5). doi: 10.3390/nano10050834.
Gupta S, Kesarla R, Omri A. Formulation Strategies to improve the bioavailability of poorly absorbed drugs with special emphasis on self-emulsifying systems. ISRN Pharmaceutics. 2013; 2013:1-16.
Erdoğar N, Esendağlı G, Nielsen TT, Esendağlı-Yılmaz G, Yöyen-Ermiş D, Erdoğdu B, Sargon MF, Eroğlu H, Bilensoy E. Therapeutic efficacy of folate receptor-targeted amphiphilic cyclodextrin nanoparticles as a novel vehicle for paclitaxel delivery in breast cancer. J Drug Target. 2018 Jan; 26(1):66-74. doi: 10.1080/1061186X.2017.1339194.
Kasinski AL, Kelnar K, Stahlhut C, Orellana E, Zhao J, Shimer E, Dysart S, Chen X, Bader AG, Slack FJ. A combinatorial microRNA therapeutics approach to suppressing non-small cell lung cancer. Oncogene. 2015; 34(27):3547–3555. [PubMed: 25174400]
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