Polyphenolic content, Antioxidant and Anti-inflammatory activities of Melon (Cucumis melo L. var. inodorus) Seeds

  • Amel Bouaziz Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Saliha Djidel Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Assia Bentaher Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria
  • Seddik Khennouf Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Abstract

The aim of this study was to estimate the content of polyphenols, flavonoids and tannins compounds and to evaluate the antioxidant activity and  the  in vitro anti-inflammatory  of    the ethanolic extract of  melon (Cucumis melo L.  var inodorus) seeds. The Folin-Ciocalteau and AlCl3 methods were applied in order to quantify the polyphenolic and flavonoids contents, respectively. However, DPPH and  β-carotene bleaching  method were applied  to evaluate the  in vitro antioxidant activity. The polyphenolic  and flavonoids contents  of    seeds  extract  were  found to be 37,10 ± 1,11 mg GAE/g of dry extract and 0,43 ± 0,27 mg QE/ g  of dry extract, respectively. Whereas the amount of tannins was  48, 30 ±  0,90 mg TAE/ g of dry extract. The scavenger effect of  seeds extract against DPPH radicals showed IC50 value of  4,13 ± 0,07 mg/ ml. The β-carotene bleaching assay indicated a strong inhibition percentage of the lipid peroxidation with value of 69,47 %. The in vitro anti-inflammatory activity was evaluated using the inhibition of   proteins denaturation revealed that the seeds extract had a moderate anti-inflammatory activity which is estimated at 49,09 % compared to the aspirin (97,73  %) at 1 mg/ ml.  Finally, melon seeds may be considered as a promising source of natural antioxidants which possess remarkable therapeutic action as inflammatory activity and may serve as food ingredients.


Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Oxidative stress,  Anti-inflammatory activity.

Keywords: Cucumis melo var. inodorus, Polyphenols, Flavonoids, Tannins, Oxidative stress, Anti-inflammatory activity

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Author Biographies

Amel Bouaziz, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of  Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Saliha Djidel, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of  Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Assia Bentaher, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of  Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Seddik Khennouf, Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

Laboratory of Phytotherapy Applied to Chronic Diseases, Department of Biology and Animal Physiology, Faculty of  Nature and Life Sciences, University Ferhat Abbas Setif 1, 19000, Algeria

References

1. Goutzourelas N , stagos D , spanidis Y , liosi M , apostolou A , priftis A , haroutounian S, spandidos D A , tsatsakis A M and Kouretas D, Polyphenolic composition of grape stem extracts affects antioxidant activity in endothelial and muscle cell, Molecular Medicine reports, 2015; 12: 5846-5856.
2. Rahman MM, Hossain MS , Mostofa M G, Ali Khan M, Ali R, Mosaddik A, Sadik M G, Khurshid Alam A , Evaluation of anti-ROS and anticancer properties of Tabebuia pallida L. Leave. Clinical Phytoscience,2019; 5:17, 1389.
3. Singh J P, Kaur A, Singh N, Nim L, Shevkani K, Kaur H, Arora DS, In vitro antioxidant and antimicrobial properties of jambolan (Syzygium cumini) fruit polyphenols. LWT-Food Science and Technology, 2016; 65: 1025-1030.
4. Rolim P M , Fidelis G P , Padilha, C E A , Santos E S, Rocha H A O , Macedo G R, Phenolic profile and antioxidant activity from peels and seeds of melon (Cucumis melo L. var. reticulatus) and their antiproliferative effect in cancer cells. Brazilian Journal of Medical and Biological Research, 2018; 51(4): 6069
5. Zeb A, Phenolic Profile and Antioxidant Activity of Melon (Cucumis Melo L.) Seeds from Pakistan, foods, 2016; 5:67-73.
6. Ismail H I , Chan KW , Mariod AA , Ismail M, Phenolic content and antioxidant activity of cantaloupe (Cucumis melo) methanolic extracts, Food Chemistry, 2010;119: 643–647.
7. Markham K R. Techniques of Flavonoid Identification, London Academic Press, Chap. 1 and 2, 1982, p. 113.
8. Li HB , Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y, Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae, Food chem,2007; 102 : 771-776.
9. Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx , Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung, 1996; 46 : 1086-1089.
10. Gharzouli K, Khennouf S, Amira S, Gharzouli A, Effects of aqueous extracts from Quercus ilex L. root bark, Punica granatum L. fruit peel and Artemisia herba-alba Asso leaves on ethanol-induced gastric damage in rats. Phytother Res, 1999; 13: 42-45.
11. Hanato T, Kagawa H, Yasuhara T, Okuda T, Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects, Chemical & Pharmaceutical Bulletin, 1998; 2090–2097.
12. Dapkevicius A, Venskutonis R, Van Beek T A, Linssen JPH. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J Sci Food Agric 1998; 77: 140-146.
13. Akkouche Z, Aissat L, Madani K, Effect of Heat on Egg White Proteins. International Conference on Applied Life Sciences, 2012; 407-413.
14. Saoudi S, Khennouf S, Mayouf N, Identification and quantification of fruit phenolic compounds of Malus communis, Journal of Drug Delivery & Therapeutics, 2019; 9(6-s):1-4.
15. Bouaziz A, Khennouf S, Abdalla S, Djidel S, Abu Zarga M, Bentahar A, Baghiani A, dahamna S, Amira S, Phytochemical analysis, antioxidant activity and hypotensive effect of Algerian azarole (Crataegus azarolus L.) leaves extracts, Res J Pharm Biol Chem Sci,2014; 5 (2) :286-305.
16. Baghiani A, Boumerfeg S, Adjadj M, Ameni D, Djermouni M, Khelifi-Touhami F, Charef N, Khennouf S, Arrar L, Antioxidants, free radicals scavenging and xanthine oxidase inhibitory potentials of Ajuga iva L. extracts. Free Radic Antioxid J, 2011; 1: 21–30.
17. Aouachriaa S, Boumerfeg S, Benslama A, Benbacha F, Guemmeza T, Khennouf S, Arrar L, Baghiani A, Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract, Journal of Ethnopharmacology, 208;2017: 105–116.
18. Banerjee S, Chanda A, Adhikari A, Das AK, and Biswas S, Evaluation of Phytochemical Screening and Anti Inflammatory Activity of Leaves and Stem of Mikania scandens (L.) Wild, Ann Med Health Sci Res, 2014; 4(4): 532–536.
19. Aichour R, Benzidane N, Arrar L, Charef N and Baghiani A, Hepatoprotective and Anti-inflammatory Activities of Algerian Capparis spinosa L, Annual Research & Review in Biology, 2018; 25(3): 1-12.
20. Sangeetha G, Vidhya R, In vitro anti-inflammatory activity of different parts of Pedalium murex (L.), Int J Herb Med, 2016;4:31-6.
21. Sostres C, Gargallo C J, Arroyo M T, Lanas A, Adverse effects of non-steroidal anti-inflammatory drugs (NSAIDs, aspirin and coxibs) on upper gastrointestinal tract. Best Pract Res Clin Gastroenterol, 2010; 24:121-32.
22. Marrassini C, Peralta I, Anesini C , Comparative study of the polyphenol content-related anti-inflammatory and antioxidant activities of two Urera aurantiaca specimens from different geographical areas , Chin Med, 2018; 13: 22.
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Bouaziz A, Djidel S, Bentaher A, Khennouf S. Polyphenolic content, Antioxidant and Anti-inflammatory activities of Melon (Cucumis melo L. var. inodorus) Seeds. JDDT [Internet]. 15Apr.2020 [cited 18Aug.2022];10(2-s):22-6. Available from: https://jddtonline.info/index.php/jddt/article/view/4022