Chemical composition, antibacterial activity of essential oil and anatomical study of Chrysanthemum morifolium

  • Habiba BOUKHEBTI Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria
  • I. Demirtas Department of Chemistry. Faculty of Natural Sciences. Cankiri Karatekin University. 18200. Cankiri. Turkey
  • L. Omar Department of Ecology and Vegetal Biology . Faculty of Nature and Life Sciences. Ferhat Abbas University Setif-1.,19000 Setif. Algeria
  • A.N. Chaker Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria

Abstract

The aim of this study is to identify the chemical composition and to evaluate the antimicrobial activity of Chrysanthemum morifolium. The analysis and identification of essential oil which obtained by hydrodistilation method were realized by gaz chromatography and mass spectroscopy. The antibacterial activity was tested by using the agar diffusion test and the Gram positive and negative pathogenic bacteria: Staphylococcus aureus ATCC 25923. Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Citrobacter freundii ATCC 8090, Kleibseilla pneumoniae ATCC 700603 and Shigella sonnei were used to evaluate this activity. This analysis led to the identification of 26 compounds representing 88.40 % of the total essential oil mass. The major compound was Verbenone (17.33 %). Other components present in appreciable contents were: Chrysanthenone (9.71%). 4-epi-cubedol (07.25%) and δ-Cadinol (05.29 %). Essential oil of Chrysanthemum morifolium exhibited an antibacterial effect against pathogenic bacteria, like those observed against Staphylococcus aureus ATCC 25923 (35±1.2mm) and Citrobacter freundii ATCC 8090 (21±0.87mm), however Pseudomonas aeruginosa  ATCC  27853  and  Kleibseilla  pneumoniae  ATCC  700603                      were resistant. The anatomical study showed the presence of several types of trichomes including the glands secreted for essential oils and protector trichomes.


Keywords: essential oil, antibacterial activity, Chrysanthemum morifolium, anatomical study, chemical composition

Keywords: essential oil, antibacterial activity, Chrysanthemum morifolium, anatomical study, chemical composition

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Author Biographies

Habiba BOUKHEBTI, Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria

Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria

I. Demirtas, Department of Chemistry. Faculty of Natural Sciences. Cankiri Karatekin University. 18200. Cankiri. Turkey

Department of Chemistry. Faculty of Natural Sciences. Cankiri Karatekin University. 18200. Cankiri. Turkey

L. Omar, Department of Ecology and Vegetal Biology . Faculty of Nature and Life Sciences. Ferhat Abbas University Setif-1.,19000 Setif. Algeria

Department of Ecology and Vegetal Biology . Faculty of Nature and Life Sciences. Ferhat Abbas University Setif-1.,19000 Setif. Algeria

A.N. Chaker, Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria

Laboratory of Natural Resource Valorization. Faculty of Nature Life Sciences. Ferhat Abbas University Setif-1. 19000, Setif. Algeria

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BOUKHEBTI H, Demirtas I, Omar L, Chaker A. Chemical composition, antibacterial activity of essential oil and anatomical study of Chrysanthemum morifolium. JDDT [Internet]. 15Apr.2020 [cited 16Aug.2022];10(2-s):7-3. Available from: https://jddtonline.info/index.php/jddt/article/view/4015