Biochemical and Toxicological Evaluations of Aqueous Extract of Parquetina nigrescens (Afzel.) leaves on Mifepristone-induced Polycystic Ovarian Syndrome in Rats

Authors

  • Fehintoluwa Joy Femi-olabisi Biochemistry Unit, Department of Biological Sciences, Mountain Top University, Ogun, Nigeria
  • Opeyemi Faokunla Department of Biochemistry, Federal University Lokoja, Kogi State, Nigeria
  • Anthonia Oluyemi Agboola Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria
  • Imoleayo Moses Olorunyolemi Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria

Abstract

The biochemical and toxicological effects of aqueous extract of Parquetina nigrescens leaves (AEPNL) at the doses of 50, 100, and 200 mg/kg body weight on mifepristone-induced in Polycystic Ovarian Syndrome (PCOS) was investigated in female Wistar rats. Sixty female Wistar rats (190.00 ± 13.00 g) were assigned into 6 groups (A - F) of ten each: animals in group A received 0.5 ml of distilled water orally on daily basis for 30 days while the mifepristonized rats in groups B, C, D and E also received orally 0.5 ml of distilled water, 7.14mg/kg of metformin (reference drug) and same volume of the extract corresponding to 50, 100, and 200 mg/kg body weight of AEPNL respectively after which levels of some biochemical and toxicological indices were determined. AEPNL aggravated mifepristone-treatment related increases in albumin, total protein and liver aspartate aminotransferase activity and mitigated the increases in globulin, total bilirubin, urea, creatinine, liver and serum alkaline phosphatase and alanine aminotransferase activities, and no treatment-related histoarchitectural changes occurred in the liver, kidney and uterus of the female rats. Therefore, the aqueous extract of Parquetina nigrescens leaves attenuated and also aggravated some biochemical parameters in the serum, liver and kidney but with no histological changes in the liver, kidney and uterus of the mifepristonized female Wistar rats.

Keywords: Mifepristone, toxicology, Parquetina nigrescens, Polycystic Ovarian Syndrome, histology

Keywords:

Mifepristone, toxicology, Parquetina nigrescens, Polycystic Ovarian Syndrome, histology

DOI

https://doi.org/10.22270/jddt.v10i2-s.3956

Author Biographies

Fehintoluwa Joy Femi-olabisi, Biochemistry Unit, Department of Biological Sciences, Mountain Top University, Ogun, Nigeria

Biochemistry Unit, Department of Biological Sciences, Mountain Top University, Ogun, Nigeria

Opeyemi Faokunla, Department of Biochemistry, Federal University Lokoja, Kogi State, Nigeria

Department of Biochemistry, Federal University Lokoja, Kogi State, Nigeria

Anthonia Oluyemi Agboola, Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria

Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria

Imoleayo Moses Olorunyolemi, Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria

Biochemistry Unit, Department of Biological Sciences, Wesley University Ondo, Nigeria

References

Joshia, S. C., Aksha, S. and Mridula, C. Antifertility potential of some medicinal plants in males: An overview. Int. J. Pharm. Pharm. Sci. 2010; 3(5):204-217

Yakubu, M. T. and Nurudeen, Q. O. Toxicological effects of aqueous extract of Cnestis ferruginea (Vahl ex DC) root in paroxetine-induced sexually impaired male rats. J. Investig. Biochem. 2014; 3(5):39-45

Ugwu, G. O., Iyoke, C. A., Onah, H. E. and Mba, S. G. Prevalence, presentation and management of polycystic ovary syndrome in Enugu, south east Nigeria. Niger. J. Med. prac. 2013; 22(4):313-316

Goldenberg, N. and Glueck, C. Medical therapy in women with polycystic ovary syndrome before and during pregnancy and lactation. Minerva Ginecol. 2008; 60(1):63-75,

Pachiappan, S., Matheswaran, S., Saravanan, P. P. and Muthusamy, G. Medicinal plants for polycystic ovary syndrome: A review of phytomedicine research. Int. J. Herb. Med. 2017; 5(2):78-80

Schlage, C. Medical plants of the wasambas (Tanzania): Documentation and Ethnopharmacological evalution. Plant Biol. 2002; 2:83-92.

Moghetti, P., Castello, R., Tosi, F., Caputo, M., Zanolin, E., Muggeo, M. and Muggeo, M. Metformin effects on clinical features, endocrine and metabolic profiles, and insulin sensitivity in polycystic ovary syndrome: a randomized, double-blind, placebo-controlled 6-month trial, followed by open, long-term clinical evaluation. J. Clin. Endocrinol. 2000; 85:139-146,

Tang, T., Lord, J. M., Norman, R. J., Yasmin, E. and Balen, A. H. Insulin sensitizing drugs (metformin, rosiglitazone, pioglitazone, D-chiro-inosital) for women with polycystic ovary syndrome, oligoamenorrhoea and sub-fertility. Cochrane Database Syst. Rev. 2012; 5:30-53

Olayinka, O. L., Eltahir, A. G. K., Atunwa, S. A., Abdullaahi, A. A., Salawu, M. K., Ogunwale, K. A. T., Olalere, F. D., Lawal, S. A., Abdulrafiu, M., Olutunde, O. A., Afolabi, O. O. and Nafiu, A. B. Safety and toxicity of aqueous leaf extracts of Camellia sinensis, Parquetina nigrescens and Telfairia occidentalis in mice. Afr. J. Pharm. Pharmaco. 2018; 12(18):208-220

Bateman, J., Chapman, R. D. and Simpson, D. Possible toxicity of herbal remedies. Scott. Med. 1998; 43:7-15

Ernst, E. Harmless herbs? A review of the recent literature. Am. J. Med. 1998; 104:170-178

Awodele, O., Amagon, K. I, Wannang, N. N, Aguiyi, J. C. Traditional medicine policy and regulation in Nigeria: an index of herbal medicine safety. Curr. Drug Safe. 2014; 9(1):16-22

Saad, B., Azaizeh, H., Abu-Hijleh, G. and Said, O. Safety of traditional arab herbal medicine. Evid.-Based Complementary Altern. Med. 2006; 3:433-439.

Chang, I. M. Toxicity of herbal drugs, International Forum on Research and Development for Procedures involving Risk Assessment of Toxic Chemicals. In: Korean Society of Toxicology. Chang, I. M and Park, C. W. (ed.s). 1987; 243-257.

Isbrucker, R. A., Edwards, J. A., Wolz, E., Davidovich, A. and Bausch, J. Safety studies on epigallocatechin gallate (EGCG) preparations. Part 2: dermal, acute and short-term toxicity studies. Food Chem. Toxicol. 2006; 44:636-650.

Irvine, F. R. Woody plants of Ghana with special reference to their uses. Oxford University Press. London. 1961; 19-20:695.

Agbor, G. A. and Odetola, A. A. Effect of Parquetina nigrescens on erythrocyte indices and serum electrolytes of rats following acute blood loss. Pak. J. Biol. Sci. 2005; 8:527–531.

Burkill, H. M. The useful plants of West Tropical Africa. 1985; 4 &8

Fong, H. H. S., Tin-Wa, M. and Farnsworth, N. R. Cytotoxic principles of Parquetina nigrescens (Afzel.) bullock (Asclepiadaceae). J. Pharm. Sci. 1995; 64(10):1674-1676.

Agbor, A. G. and Odetola, A. A. Hematological studies of Parquetina nigrescens haemorrhagic anaemic rats. Afr. J. Med. Med. Sci. 2001; 30:105-109.

Kayode, O. T. and Yakubu, M. T. Parquetina nigrescens leaves: chemical profile and influence on the physical and biochemical indices of sexual activity of male Wistar rats. J. Integr. Med. 2017; 15(1):64-76

Saba, A. B., Oyagbemi, A. A. and Azeez, O.I. Antidiabetic and haematinic effects of Parquetina nigrescens on alloxan induced type-1 diabetes and normocytic normochromic anaemia in Wistar rats. Afr. Health Sci. 2010; 10(3):276-282

Datté a Jacques, Y., Zieglera, A. and Tillementb, J. Sympathomimetic effects of Parquetina nigrescens (Periplocaceae) extract in isolated portal vein smooth muscle. Gen. Pharmacol. 1999; 32(5):551-556

Yakubu, M. T., Olawepo, F. J., Olayaki, L. A. and Ibrahim, O. O. K. Mifepristone (RU486) induces polycystic ovarian syndrome in female Wistar rats with features analogous to humans. J. Endocrinol. Reprod. 2015; 19(1):40-51

Yakubu, M. T., Akanji, M. A., Oladiji, A. T., Olatinwo, A. O., Adesokan, A. A., Yakubu, M. O., Owoyele, B. V., Sunmonu, T. O. and Ajao, M. S. Effect of Cnidoscolous aconitifolius (Miller) I.M. Johnston leaf extract on reproductive hormones of female rats. Iran J. Reprod. Med. 2008; 6(3):149-155

Tietz, N. W., Pruden, E. L. and Siggaard-Anderson, O. Tietz Textbook of Clinical Chemistry. In: Burtis, C. A., and Ashwood, E. R. (ed.s). W. B. Saunders Company, London. 1994; 1354-1374.

Krause, W. J. The Art of Examining and Interpreting Histologic Preparations. A Student Handbook. Partheton Publishing Group, United Kingdom. 2001; 9-10, 24

Drury, R. A. and Wallington, E. A. In: Carleton’s Histological Techniques. 4th ed. Oxford University Press, New York. 1973; 58

Yakubu, M. T., Bilbis, L. S., Lawal, M. and Akanji, M. A. Effect of repeated administration of sildenafil citrate on selected enzyme activities of liver and kidney of male albino rats. Niger. J. Pure Appl. Sci. 2003; 18:1395-1400

Ganong, W. F. Review of Medical Physiology. 20th ed. Lange Medical Books, London. 2001; 414-417.

Kaplan, A., Szabo, L. V. L. and Opheim, K. E. Clinical Chemistry: Interpretation and Techniques: Lea and Febiger Philadelphia. xvii, 1979; 440 p. : ill.

Taubert, D., Roesen, R. and Schömig, E. Effect of cocoa and tea intake on blood pressure: meta-analysis of randomized controlled trials. Arch. Intern. Med. 2007; 167(7):626-634

Shaffiee, M. A., Akbarian, F., Memon, K. K., Aarabi, M. and Boroumand, B. Dermatologic Manifestations in End-stage Renal Disease. Iran J. Kidney Dis. 2015; 9(5):1-15

Shahjahan, M., Sabitha, K. E., Jamu, M. and Shyamala-Dev, C. S. Effect of Solanum trilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J. Med. Res. 2004; 120:194-198

Akanji, M. A., Olagoke, O. A and Oloyede, O. B. Effect of chronic consumption of metabisulphite on the integrity of the rat kidney cellular system. Toxicology. 1993; 81:173-179.

Umezawa, H. and Hooper, I. R. eds. “Aminoglycoside Antibiotics” Handbook on Experimental Pharmacology. Springer-Verlag, Berlin and New York. 1982; vol 62,

Owolabi, J. O. and Ogunnaike, P. O. Histological evaluation of the effects of Moringa leaf extract treatment on vital organs of murine models. Merit Res. J. Med. Med. Sci. 2014; 2(10):245-257

Published

15-04-2020
Statistics
Abstract Display: 694
PDF Downloads: 642

How to Cite

1.
Femi-olabisi FJ, Faokunla O, Agboola AO, Olorunyolemi IM. Biochemical and Toxicological Evaluations of Aqueous Extract of Parquetina nigrescens (Afzel.) leaves on Mifepristone-induced Polycystic Ovarian Syndrome in Rats. J. Drug Delivery Ther. [Internet]. 2020 Apr. 15 [cited 2025 Feb. 14];10(2-s):94-101. Available from: https://jddtonline.info/index.php/jddt/article/view/3956

How to Cite

1.
Femi-olabisi FJ, Faokunla O, Agboola AO, Olorunyolemi IM. Biochemical and Toxicological Evaluations of Aqueous Extract of Parquetina nigrescens (Afzel.) leaves on Mifepristone-induced Polycystic Ovarian Syndrome in Rats. J. Drug Delivery Ther. [Internet]. 2020 Apr. 15 [cited 2025 Feb. 14];10(2-s):94-101. Available from: https://jddtonline.info/index.php/jddt/article/view/3956