Comparative Antiproliferative activity of aerial parts of few Apocynaceae plants in HepG2, HT29 and SK-OV3 Human cancer cell lines

  • Nirmala Devi Institute of Pharmacy, Bundelkhand University, UP, INDIA
  • Ajay Kumar Gupta University Institute of Pharmacy, CSJM University, UP, INDIA
  • Sunil Kumar Prajapati Institute of Pharmacy, Bundelkhand University, UP, INDIA


Aims: Apocynaceae family is the 5th largest medicinal plant family rich in potent secondary metabolites such as Alkaloids, Cardiac glycosides,
Terpenoids, irridoid/secoirridoids, flavonoids and Phenolic contents. The present study was aimed to evaluate and compare in-vitro
antiproliferative activity of three plants of this family.
Methods: Aerial parts of Carissa carandas Linn. (C), Nerium indicum Mill. (N) and Wrightia tinctoria RBr. (W), were collected and dried. The
powdered drugs were extracted in Ethanol (1), 60% Ethanol (2) and Water (3). Estimation of Phytoconstituents performed using standard
methods. In-vitro cytotoxic activity performed using Sulphorhodamine B (SRB) assay in HepG2, HT29 and SKOV3 human cancer cell lines taking
Adriamycin (ADR) as standard. For extracts, GI50 value ≤ 20μg/ml was considered to demonstrate activity.
Results: For HepG2 cell line graphs and photomicrographs showed GI50 value as ADR=39.79, C1=2.5, N2=66.3, N3<10 and C2=C3= N1=W1-
3>80. Also TGI for C1>80. The extracts, C1, C2, N1, N2, and N3 were found to possess activity against HepG2.These extracts were screened on
HT 29 and SKOV3cell lines. The GI50 value observed was<10 for C1, N2, N3 and ADR in HT 29 and <10 for N3 and ADR in SK OV3 cell lines.
Thus it was found that aqueous extract of Nerium indicum (N3) and Ethanolic extract of Carissa carandas (C1) were most cytotoxic extracts
against all three cell lines.
Conclusions: our study establishes that Apocynaceae family plants could be an important anticancer lead and could serve as Botanical drug for
Keywords: Apocynaceae, SRB Assay, Phytoconstituents, Anticancer drug screening models, Hep G2, HT 29, SK-OV3, HCC.

Keywords: Apocynaceae, SRB Assay, Phytoconstituents, Anticancer


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Author Biographies

Nirmala Devi, Institute of Pharmacy, Bundelkhand University, UP, INDIA

Institute of Pharmacy, Bundelkhand University, UP, INDIA

Ajay Kumar Gupta, University Institute of Pharmacy, CSJM University, UP, INDIA

University Institute of Pharmacy, CSJM University, UP, INDIA

Sunil Kumar Prajapati, Institute of Pharmacy, Bundelkhand University, UP, INDIA

Professor, Institute of Pharmacy, Bundelkhand University, UP, INDIA


1. Organization WH. Noncommunicable Diseases Country Profiles 2018. Geneva: World Health Organization; 2018.
2. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine World Health Organization. 2000.
3. Mallath, MT et al. The growing burden of cancer in India: epidemiology and social context. Lancet Oncol. 2014:e205-e212.
4. Alberts Bruce, Roberts Keith. Molecular Biology of the Cell, 5th edn. New York USA and Abingdon UK.: Garland science, Taylor and Francis group; 2008:1205-1264.
5. Plummer M, C. d. (2016). Global burden of cancers attributable to infections in 2012: a synthetic analysis. The Lancet Global Health, e609-e616.
6. Ray, Arunabha; Gulati K. Recent Advances in Herbal Drug Research. First. (Ray, Arunabha; Gulati K, ed.). New Delhi: I.K. International Publishing House Ltd.; 2010.
7. Farnsworth, NR; Kaas CJ. An approach utilizing information from traditional medicine to identify tumor-inhibiting plants. J Ethnopharmacol. 1981:85-99.
8. Pan L, Chai H, Kinghorn AD. The continuing search for antitumor agents from higher plants. Phytochem Lett 2010; 3:1–8.
9. Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. Journal of Ethnopharmacology J Ethnopharmacol 2005; 100(1-2): 72-79.
10. Weng CJ, Yen GC. Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: Phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treatment Reviews 2012, 76–87.
11. Bhadane BS, Patil MP, Maheshwari VL, Patil RH. Ethnopharmacology, phytochemistry, and biotechnological advances of family Apocynaceae: A review. Phyther Res. 2018; 32(7):1181-1210.
12. Mahbubur Rahman AHM, Akter M. Taxonomy and traditional medicinal uses of apocynaceae (Dogbane) family of Rajshahi District, Bangladesh. Int J Bot Stud. 2016; 1(2):05-13.
13. Nadkarni, KM; Nadkarni A. Indian Materia Medica. Bombay: Bombay Popular Prakashan.; 1976.
14. Garbett NC, Graves DE. Extending nature’s leads: The anticancer agent ellipticine. Curr Med Chem - Anti-Cancer Agents. 2004; 4(2):149-172.
15. Warrier P K, Nambiar V P K RC. Indian Medicinal Plants: a compendium of 500 species. In: vaidya ratnam’s PS V, ed. Arya Vaidya Sala. first, Vol. Kottakkal: universities press; 386, 126, 417.2010
16. Pandey Gyanendra. Materia Medica-Vegtable Drugs. In: Gyanendra P, ed. Dravyaguna Vijnana. first. Varanasi: Krishnadas Academy; 2001:141-147, 123-127, 370-380.
17. Kirtikar KR. Apocynaceae. . Indian Medicinal Plants, 2nd edn. Allahabad: Lalit Mohan Basu, Allahabad; 1935:1546-1549, 1581-1584, 1584-1586.
18. Devi N, Jain SK. Evaluation of Hepatoprotective property of Ethanolic extrct of Carissa Carandas Linn. in CCl4 intoxicated Rats. Inven Rapid - Ethnopharmacol. 2013:1-7.
19. Devi N et al. Indian tribe’s and villager’s health and habits: Popularity of apocynaceae plants as medicine. Int J Green Pharm. 2017; 11(2):S256-S279.
20. Devi N et al. Prospects of Traditionally important Apocynaceae plants of India in Cancer Remediation. J Drug Deliv Ther. 2019; 9(1):293-302.
21. Kaunda JS, Zhang YJ. The Genus Carissa: An Ethnopharmacological, Phytochemical and Pharmacological Review. Nat Products Bioprospect. 2017; 7(2):181-199.
22. Sharma P et al. Chemical Constituents of Plants from the Genus Nerium. Chem Biodivers. 2010:1198-1207.
23. Vijayavergia R, Kumar J. Quantification of primary metabolites of Nerium indicum Mill. Asia J. Experim. Sci., 21, 2007, 123-128.
24. Pathak S et al. AnvirzelTM, an extract of Nerium oleander, induces cell death in human but not murine cancer cells. Anticancer Drugs. 2000; 11(6):455-463.
25. Antony J et al. DW-F5: A Novel Formulation against Malignant Melanoma from Wrightia Tinctoria. Scientific Reports. India and USA; 2015.
26. Heinrich M, Bremner P. Ethnobotany and Ethnopharmacy - Their Role for Anti-Cancer Drug Development. Curr Drug Targets. 2006; 7(3):239-245.
27. Luque de Castro MD, Garcia Ayuso LE. Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. Anal Chim. 1998; 369(1-2):1–10.
28. Harborne, J.B. Phytochemicals Methods. Chapman and Hall Ltd., London 1973, 49- 188.
29. Evanse, T. (2009). Phytochemistry. In W. C. Evans, Pharmacognosy (pp. 133-148). London: Saunders Elsevier.
30. Boyd MR. The NCI In Vitro Anticancer Drug Discovery Screen. Anticancer Drug Dev Guid. 1997; 23-42.
31. Skehan P, Storeng R, Scudiero D, Monks A, Mcmahon J, Vistica D, Warren J, Bokesch H, Kenney S BM. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990; 82(13):1107-1112.
32. Vichai V KK. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc. 2006; 1(3):1112-1116.
33. Briskin DP. Medicinal plants and phytomedicines. Linking plant biochemistry and physiology to human health. Plant Physiol. 2000; 124(2):507-514.
34. Thun MJ et al. The global burden of cancer: priorities for prevention. Carcinogenesis, Carcinogenesis 2010, 31(1):100-110.
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Devi N, Gupta AK, Prajapati SK. Comparative Antiproliferative activity of aerial parts of few Apocynaceae plants in HepG2, HT29 and SK-OV3 Human cancer cell lines. JDDT [Internet]. 25Aug.2019 [cited 18Jun.2021];9(4-s):1195-202. Available from: