A promising cognition boosting effect of leaves of Abrus precatorius

  • Gajendra Pratap Choudhary School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore, Madhya Pradesh 452001, India
  • Ashutosh Pal Jain School of Pharmacy, Devi Ahilya Vishwavidyalaya, Indore, Madhya Pradesh 452001, India


Objective: To assess the leaves of Abrus precatorius had earlier show cognition boosting effect against dementia in rodents. Methods: The methanol extract was fractionated into ethyl acetate and aqueous fractions and assessed for its nootropic and learning activity in albino rodents. The effect of triterpenoids containing ethylacetate soluble fraction of methanol extract significant enhancement in the retention ability of amnesic mice as compared to their respective controls using elevated plus maze and Cook and Weidley’s pole apparatus. Subsequently, the extracts were further studied for its in vitro acetylcholinesterase (AChE) inhibitory potential which can correlate with an improvement in cholinergic transmission. Piracetam was used as the standard drug while scopolamine hydrobromide served as the amnestic agent. Result: A.precatorius ethylacetate (APEA) soluble fraction of leaves produced a significant acquisition, retention and retrieval at dosed 150 and 300 mg/kg in interoceptive as well as exteroceptive memory models of rodents. While A.precatorius aqueous fraction (APAqs) of methanol extract not enhances both the acquisition as well as the retention of memory of learned task when administered at a dose of 300 mg/kg for a period of 7 days. It was found that APEA fraction potentially inhibits AChE with percentage inhibition of 91.33±0.33. Furthermore, both fraction were found safe with no deaths in mice treated orally with 2000 mg/kg.

Conclusion: APEA showed to be a useful memory restorative agent in the treatment of dementia from the experiments performed.

Keywords: Abrus precatorius, Piracetam, Scopolamine, Dementia


Download data is not yet available.


1. LeMerrer J, Noguès X. Cognitive neuropharmacology: New perspectives for the pharmacology of cognition, Pharmacological Research, 2000; 41: 503–514.
2. Barbara JS, Sharon MZ. Neuroethical Issues in Cognitive Enhancement, Journal of Psychopharmacology 2011; 25(2):197–204.
3. Giurgea C. The nootropic approach to the pharmacology of the integrative action of the brain, Cond Reflex, 1973; 8:108–115.
4. Hannan MA, Kang JY, Mohibbullah M, Hong YK, Lee H, Choi JS, Choi IS, Moon IS. Moringa oleifera with promising neuronal survival and neurite outgrowth promoting potentials, Journal of Ethno pharmacology, 2014; 152:142–150.
5. Ojha R, Sahu AN, Muruganandam AV, Singh G K , Krishnamurthy S. Asparagus racemosus enhances memory and protects against amnesia in rodent models, Brain and Cognition,2010; 74:1–9.
6. Vollala VR, Upadhya S, Nayak S.Effect of Bacopa monniera Linn. (Brahmi) extract on learning and memory in rats: A behavioral study, Journal of Veterinary Behavior, 2010; 5: 69-74.
7. Nahata A, Patil UK, Dixit VK. Effect of Evolvulus alsinoides Linn. on Learning Behavior and Memory Enhancement Activity in Rodents, Phytotherapy Research,2010; 24: 486–493.
8. Rao SB, Chetana M, Devi PU. Centella asiatica treatment during postnatal period enhances learning and memory in mice, Physiology & Behavior, 2005; 86:449 – 457.
9. Soman I, Mengi SA, Kasture SB. Effect of leaves of Butea frondosa on stress, anxiety, and cognition in rats, Pharmacology Biochemistry and Behavior, 2004; 79:11– 16.
10. Zore GB, Award V, Thakre AD, Halad UK, Meshram NS, Surwase BS, et al. Activity-directed-fractionation and isolation of four antibacterial compounds from Abrus precatorius roots, Natural Product Research,2007; 21(9): 838-845.
11. Ghosh D, Maiti TK. Immunomodulatory and anti-tumor activities of native and heat denatured Abrus agglutinin, Immunobiology, 2007; 212(7): 589-599.
12. Ramnath V, Kuttan G, Kuttan R. Immunopotentiating activity of abrin, a lectin from Abrus precatorius Linn,Indian journal of experimental biology,2002; 40(8): 910-913.
13. Ratnasooriya WD, AS Amarasekera, NSD Perera, GAS Premakumara.Sperm antimotility properties of a seed extract of Abrus Precatorius, Journal of Ethnopharmacol 1991; 33(2): 85–90.
14. Rao MV. Anti-fertility effects of alcoholic seeds extract of Abrus Precatorius Linn. in male albino rats. Acta Europaea fertilitatis, 1987; 18(3):217–220.
15. Taur DJ, Patil RN, Patil RY. Antiasthmatic related properties of Abrus precatorius leaves on various models, Journal of Traditional and Complementary Medicine, 2017; 30:1-5.
16. Georgewill OA, Georgewill UO. Evaluation of the anti-inflammatory activity of extract of Abrus precatorious, Eastern Journal of Medicine, 2009; 14: 23-25.
17. Anam EM. Anti-inflammatory activity of compounds isolated from the aerial parts of Abrus precatorius (Fabaceae), Phytomedicine, 2001; 8(1): 24-27.
18. Nwodo OF, Alumanah EO. Studies on Abrus precatorius seeds. II: antidiarrhoeal activity, Journal of Ethnopharmacology, 1991; 31(3): 395-398.
19. Monago CC, Alumanah EO. Anti-diabetic Effect of Chloroform -Methanol Extract of Abrus Precatorius Linn Seed in Alloxan Diabetic Rabbit. Journal of Applied Sciences and Environmental Management, 2005; 9 (1): 85 – 88.
20. Wakawa HY, Oluwayemisi T, Saadatu A A, Fasihuddin BA. Effects of Aqueous Extract of Leaves of Abrus precatorius (Linn) on some Biochemical Parameters in Normal and Alloxan Induced Diabetic Rats,Asian Journal of Biochemistry,2017; 12(2):44-48.
21. Adesina SK. Studies on some plants used as anticonvulsants in Amerindian and African traditional medicine, Fitoterapia, 1982; 53:147–162.
22. Pal S, Ariharasivakumar G, Girhepunje K, Upadhyay A. In-Vitro Antioxidative activity of Phenolic and Flavonoid compounds extracted from Seeds of Abrus Precatorius. International Journal of Pharmacy and Pharmaceutical Sciences, 2009; 1(2):136-140.
23. Palvai VR, Mahalingu S, Urooj A. Abrus precatorius Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability, International Journal of Medicinal Chemistry Volume,2014; 1-7.
24. Acharya D. Medicinal plants for curing common ailments in India, Positive Health, 2004; 102: 28-30.
25. Kirtikar KR, Basu BD. Indian medicinal plant Vol I. 2nd ed. Dehradun: International Book Distributors; 2005.
26. Nadkarni KM. Indian materia medica Vol-I. Mumbai: Popular Prakashan; 1994, p. 4-7.
27. Khandelwal KR. Practical pharmacognosy techniques and experiments. Pune:Nirali prakashan; 2008,19:149-153.
28. Dixon WJ. The up and down methods for small animals,Journal of the American Statistical Association,1965; 60: 967-978.
29. Ecobichon DJ. The Basis of Toxicology Testing. CRC Press, New York1997: pp 43–86.
30. Sharma AC, Kulkarni SK. Evaluation of learning and memory mechanisms employing elevated plus-maze in rats and mice, Progress in Neuro- Psychopharmacology and Biological Psychiatry, 1992; 16:117–125.
31. Parle M, Dhingra D, Kulkarni SK. Improvement of mouse memory by Myristica fragrans seeds, Journal of Medicinal Food, 2004; 7:157-161.
32. Cook L, Weidley E. Behavioral effects of some psychopharmacological agents, Annals of the New York Academy of Sciences, 1957; 66:740–752.
33. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochemical Pharmacology, 1961; 7: 88–95.
34. Dhanasekaran S, Perumal P, Palayan M. In-vitro Screening for acetylcholinesterase enzyme inhibition potential and antioxidant activity of extracts of Ipomoea aquatica Forsk: therapeutic lead for Alzheimer’s disease,Journal of Applied Pharmaceutical Science,2015; 5 (02):12-16.
35. Bhattacharya SK, Upadhyay SN, Jaiswal AK. Effect of piracetam on electroshock induced amnesia and decrease in brain acetylcholine in rats, Indian journal of experimental biology 1993; 31:822–826.
36. Parle M, Dhingra D, Kulkarni SK.Neuromodulators of learning and memory, Asia-Pacific Journal of Pharmaceutical Sciences, 2004; 16:89–99.
37. Ghelardini C, Galeotti N, Barboloni A, Furukawa S. Memory facilitation and stimulation of endogenous nerve growth factor synthesis by the acetylcholine releaser PG-9,Japanese Journal of Pharmacology,1998; 78:245–51.
38. Hollander E, Mohs RC, Davis KS. Cholinergic approaches to the treatment of Alzheimer’s disease, British Medical Bulletin, 1986; 42: 97– 100.
39. Pilch H, Muller WE. Piracetam elevates muscarinic cholinergic receptor density in the frontal cortex of aged but not of young mice, Psychopharmacology (Berl), 1988; 94:74–78.
40. Itoh J, Nabeshima T, Kameyama T. Utility of an elevated plus maze for the evaluation of memory in mice: effects of nootropics, scopolamine and electroconvulsive shock, Psychopharmacology (Berl), 1990; 101: 27–33.
41. Kameyama T, Nabeshima T, Noda Y. Cholinergic modulation of memory for step-down type passive task in mice. Research communications in psychology, psychiatry and behavior, 1986; 11: 193-205.
42. Kulkarni SK, Sharma AC. Benzodiazepines and cognition, learning and memory, Drugs of Today, 1990; 26: 541-545.
43. Joshi H, Parle M. Brahmi rasayana improves learning and memory in mice, Evidence-based Complementary and Alternative Medicine,2006; 3(1):79–85.
44. Ojha R, Sahu AN, Muruganandam AV, Singh GK, Krishnamurthy S. Asparagus racemosus enhances memory and protects against amnesia in rodent models. Brain and Cognition, 2010; 74: 1-9.
45. Vasudevan M, Parle M. Memory enhancing activity of Anwala churna (Emblica officinalis Gaertn.): an Ayurvedic preparation, Physiology & Behavior, 2007; 91: 46-54.
46. Das A, Shanker G, Nath C, Pal R, Singh S, Singh HK. A comparative study in rodents of standardized extracts of Bacopa monniera and Ginkgo biloba anticholinesterase and cognitive enhancing activities, Pharmacology Biochemistry and Behavior, 2002; 73: 893–900.
47. Bhardwaj DK, Bisht MS, Mehta CK. Flavonoids from Abrus precatorius. Phytochemistry, 1980; 19: 2040-2041.
48. Akinloye BA, Adalumo LA. Abrus precatorius leaves-a source of glycyrrhizin, Nigerian Journal of Pharmaceutical Research, 1981; 12: 405.
49. Ragasa CY, Lorena GS, Mandia EH, Raga DD, Shen CC. Chemical constituents of Abrus precatorius, American Journal of Essential Oils and Natural Products,2013; 1(2):7-10.
50. Anam EM. Anti-inflammatory activity of compounds isolated from the aerial parts of Abrus precatorius (Fabaceae) Phytomedicine, 2001; 8(1), 24-27.
77 Views | 48 Downloads
How to Cite
Choudhary GP, Jain AP. A promising cognition boosting effect of leaves of Abrus precatorius. JDDT [Internet]. 16Jan.2020 [cited 14Jun.2021];9(4-s):1177-82. Available from: https://jddtonline.info/index.php/jddt/article/view/3835