Development and validation of UV Spectrophotometric method for simultaneous estimation of Lamivudine and Tenofovir Disproxil Fumarate in the combined dosage form

  • Raghvendra Singh Bhadauria Shrinathji Institute of Pharmacy, Nathdwara, Rajasthan, India
  • Rakesh Kumar Gupta Department of Pharmaceutical Sciences, Sunrise University, Alwar, Rajasthan, India

Abstract

A Simple, precise, accurate and economical spectrophotometric method was developed and validated for simultaneous estimation of Lamivudine (LAM) and Tenofovir disoproxil fumarate (TDF) in combined  dosage form. In simultaneous equation method, LAM and TDF were quantified using their absorptivity values at selected wavelengths, viz., 276nm and 260nm respectively in water solvent system. LAM and TDF obeyed Beer’s law in the concentration range of 5-25μg/ml. Different analytical parameters such as linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were determined as per ICH guidelines (Q2A & Q2B). % Recovery for both the drugs were in the range of 98.31-98.94%  indicating excellent accuracy. The methods were precise, with a relative standard deviation of less than 2% for both drugs. The simultaneous equation method permits simple, rapid and direct determination of LAM and TDF in commercially available combined dosage form without previous separations and can therefore be used for routine analysis.


Keywords: Lamivudine, Tenofovir disoproxil fumarate, Spectrophotometric analysis, Simultaneous equation method.

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Bhadauria RS, Gupta RK. Development and validation of UV Spectrophotometric method for simultaneous estimation of Lamivudine and Tenofovir Disproxil Fumarate in the combined dosage form. JDDT [Internet]. 7Jan.2020 [cited 14Jun.2021];9(4-s):1156-9. Available from: https://jddtonline.info/index.php/jddt/article/view/3827