Phytochemical Screening and Assessment of Adhatoda Vasica (Leaf) For Antihistaminic Activity
Abstract
Biologically active compounds from natural sources are of interest as possible new drugs for different diseases. Over many centuries humans have been mining the bounties of nature for discovering natural products that have been used for the treatment of all human diseases. Adhatoda vasica Nees (Acanthaceae) is widely used in the Indian system of medicine as an Asthma, bleeding, bronchitis, cough, diarrhea, dysentery, fever, flu, hysteria, neuralgia, rheumatic pain, swelling, TB, urinary disorders, vomiting etc. The plant was also reported for its diabetes, epilepsy, skin disorders and many more such activities. Clonidine, a α2 adrenoreceptor agonist induces dose dependent catalepsy in mice, which was inhibited by histamine H1 receptor antagonists but not by H2 receptor antagonist. Clonidine releases histamine from mast cells which is responsible for different asthmatic conditions. In present study methanol extract of Adhatoda vasica leave at doses 100and 200 mg/kg i.p were evaluated for antihistaminic activity using clonidine induced catalepsy in mice. Acute toxicity of the extract (2000 mg/kg) was examined in mice for 7 days. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. Phytochemical analysis revealed the presence of carbohydrates, glycosides, alkaloids, saponins, flavonoids, tannin and phenolic compounds. Methanolic extract up to 2000 mg/kg did not produce any toxic effects. The methanolic extract of leave of Adhatoda vasica (100, 200 mg/kg, p.o.) and chlorpheniramine maleate (10 mg/kg, i.p.) significantly inhibited (**P< 0.05) clonidine-induced catalepsy in mice at 180 min after the administration of clonidine. The methanolic extract of leave of Adhatoda vasica possesses significant antihistaminic activity (H1-antagonist) and can be attributed to bronchodilating, anti-inflammatory, adaptogenic activity etc. Hence detailed study needs to be conducted to evaluate the phytoconstituent responsible for the above mentioned results and their clinical efficacy in the treatment of related diseases.
Keywords: Adhatoda vasica, Clonidine, Antihistaminic activity, Chlorpheniramine maleate, Qualitative analysis.
DOI
https://doi.org/10.22270/jddt.v9i4-s.3785References
Gupta AK, Tandon N. Reviews on Indian Medicinal Plants Vol. 1, Indian Council of Medical Research. New Delhi, India. 2004; 257.
Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants Vol. 2, Central Drug Research Institute, Lucknow and National Institute of Science Communication, New Delhi, India. 1999; 14.
Anonymous. The Wealth of India, Council of Scientific and Industrial Research, New Delhi, India. 1985; 76.
Patel PK, Venkatakrishna-Bhatt H. In vitro study of antimicrobial activity of Adhatoda vasica (leaf extract) on gingival inflammation a preliminary report. Indian J Med Sci1984; 38: 70-72.
Singh RP, Padmavathi B, Rao AR. Modulatory influence of Adhatoda vesica (Justicia adhatoda) leaf extract on the enzymes of xenobiotic metabolism, antioxidant status and lipid peroxidation in mice. Mol Cell Biochem 2000; 213: 99-109.
Chakraborty A, Brantner AH. Study of alkaloids from Adhatoda vasica Nees on their antiinflammatory activity. Phytother Res 2001; 15: 532-534.
Biswas K, Kumar A, Babaria BA, Prabhu K, Setty SR. Hepatoprotective effect of leaves of Peltophorum pterocarpum against paracetamol Induced acute liver damage in rats. J Basic Clin Pharm 2009; 1:10-5.
Benicio MH, Ferreira MU, Cardoso MR, Konno SC, Monteiro CA. Wheezing conditions in early childhood: Prevalence and risk factors in the city of São Paulo. Brazil: Bull World Health Organiz; 2004; 82:516-22.
Peden DB. The epidemiology and genetics of asthma risk associated with air pollution. J Allergy Clin Immunol 2005; 115:213-9.
Mukherjee PK. Quality Control of Herbal Drugs, 2nd Edition, Business Horizons, 2007; 2-14.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 42nd ed. Pune: Nirali Prakashan; 2008.
Ferre S, Guix T, Prat G, Jane F, Casas M. Is experimental catalepsy properly measured. Pharmacol Biochem Behav 1990; 35:753-7.
Kumar D, Bhujbal SS, Patil PS, Buge PV. In-vitro and in-vivo activities of stem bark of methanolic extract of Ailanthus excelsa Roxb. In the management of asthma. Int J Pharmacol 2010; 6:284-9.
Published
Abstract Display: 1267 
PDF Downloads: 935 How to Cite
Issue
Section
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
How to Cite
.