Anti-Oxidant Activity of Ethanolic Fruits Extract of Scindapsus officinalis (Roxb)
The in-vitro antioxidant activities of the fruits of Scindapsus Officinalis (Roxb) (Araceae). The fruits of the plant were shade dried with the help of grinder was subjected to successive soxhlet extraction with Petroleum Ether and 90% v/v Ethanol. The extracts were screened for quantitative estimation of total flavonoids, tannins, total phenols, and alkaloids. Effect of extracts of Scindapsus officinalis Roxb in 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method was evaluated for in-vitro antioxidant activities. The quantity of DPPH reduced could be quantified by measuring a decrease in absorbance at 517 nm. The IC50 value was found to be 45.21and 40.11μg/ml for ethanolic extracts while the IC50 value of ascorbic acid was 18.53μg/ml significantly reduced DPPH radical by bleaching it. The ethanolic extract was found to be near to standard compounds and brought about significant antioxidant potential. This was due to the presence of one or more phytoconstituents present in ethanol extract.
Keywords: Scindapsus Officinalis (Roxb); DPPH; antioxidant, ascorbic acid.
2. Clark, E.P. and Collip, J.B. (1925), A study of the Tisdall method for the determination of blood serum calcium with a suggested modification, Biochem., 63:461-463.
3. Muller, F.L., Lustgarten, M.S, Jang, Y., Richardson, A., Van R. H., 2007. Trends in oxidative aging theories. Free Radical Biology and Medicine 43 (4), 477–503.
4. Ran, Q., Liang, H., Ikeno, Y., 2007. Reduction in Glutathione Peroxidase 4 Increases Life Span through Increased Sensitivity to Apoptosis. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences 62 (9), 932–942.
5. Scholz, R.W., Graham, K.S., Gumpricht, E., Reddy, C.C., 1989. Mechanism of interaction of vitamin E and glutathione in the protection against membrane lipid peroxidation. Annals of the New York Academy of Sciences 570, 514-517.
6. Lewis, P. et al., 2007. Lack of the Antioxidant Enzyme Glutathione Peroxidase-1 Accelerates Atherosclerosis in Diabetic Apolipoprotein E–Deficient Mice. Circulation 115, 2178-2187.
7. Fridovich, I., 1995. Superoxide radical and superoxide dismutases. The Annual Review of Biochemistry 64, 97–112.
8. Archibald, F.S., Fridovich, I., 1982. The scavenging of superoxide radical by manganous complexes: in vitro. Archives of Biochemistry and Biophysics 214, 452–463.
9. Groner, Y., 1994. Cell damage by excess CuZn SOD and Down's syndrome. Biomedicine & Pharmacotherapy 48, 231-240.
10. Gawloski, T., Bernd, S., Ruth, R., 2009. Advanced glycation end products strongly activate platelets. European Journal of Nutrition 48(8), 475-481.
11. Sundaram, R.K, Anusha, B., Selvamani, V., 1996. Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complications. Clinical Science 90, 255-260.
12. Hatori, Y., Kawasaki, K., Abe M., 1991. Superoxide dismutase altered endothelium dependent relaxation in the rat. The American Journal of Physiology - Heart and Circulatory Physiology 261, 1084-1094.
13. Renu, A., Kowluru, L.A., Yeh, S.H., 2006. Role of mitochondrial superoxide dismutase in development of diabetic retinopathy. Investigative ophthamology and visual science 47, 1594-1599.
14. Bert, S., De B.L., 2007. Impact of oxidative stress on endothelial dysfunction of children and adolescents with type 1 diabetes mellitus: Protection by superoxide dismutase. Pediatric Research 62(4), 456-461.
15. Curr. Sci., 83 :30-38.
16. Fong, S.D., Alello, L., Gardner, W.T., King, L.G., Blankenship, G., Cavallerano, D.J., Ferris, L.F. and Klein, R. (2004), Retinopathy in diabetes, Diabetes Care, 27:S84-S87.
17. Hippisley-Cox, J. and Pringle, M. (2004), Prevalence, care and outcomes for patients with diet-controlled diabetes in general practice: crosssectional survey, Lancet, 363 :423-428.
18. Kamalakannan, N. and Prince, S.M.P. (2003), Effect of Aegle marmelos Correa (Bael) fruit extract on tissue antioxidants in streptozotocin diabetic rats, Indian J. Exp. Biol., 41:1285-1288.
19. Karthikeyan, J. and Rani, P. (2003), Enzymatic and non-enzymatic antioxidants in selected Piper species, Indian J. Exp. Biol., 41: 135-140.
20. Koida, H. and Oda, T. (1959), Pathological occurrence of glucose-6- phosphatase in liver disease, Clin. Chem. Acta, 4:554-561.
21. Srinivasan, K., Muruganadhan, S. and Lal, J. (2001 ), Evaluation of anti• inflammatory activity of Pongamia pinnata leaves m rats, J. Ethnopharmacol., 78:151-157.
22. Vessby, J., Basu, S., Mohsen, R., Berne, C. and Vessby, B. (2002), Oxidative stress and antioxidant status in type 1 diabetes mellitus, Blackwell Science Ltd., JIM, 251 :69- 76.
23. Zafrilla, P., Mulero, J., Xandri, J.M., Santo, E., Caravaca, G. and Morillas, J.M. (2006), Oxidative stress in Alzheimer's disease in different stages of the disease, Curr. Med. Chem., 13:1075-1083
24. Rotruck, J.T., Pope, A.L., Ganther, H.E. and Swanson, A.B. (1984), Selenium: Biochemical roles as a component of glutathione peroxidase, Science, 179:588-590.
25. Luo, J .Z. and Luo, L. (2008), Ginseng on hyperglycemia: effects and mechanisms, eCAM Advance Access, 1-5.
26. Fiske, C.H. and Subbarow, Y. (1925), The colorimetric determination of phosphorus, J. Biol. Chem., 66:375-400.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).