ERIC-PCR: A Molecular Typing Tool for Genotyping Multi Drug Resistant Pseudomonas aeruginosa Isolated from the Pus Samples

  • Deepak Kumar Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar-608002, India
  • S Sivakumar Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India
  • P Sharmila Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India
  • K Sivasubramani Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India

Abstract

Pseudomonas aeruginosa is a typical and major human pathogen, it causes numerous risky opportunistic infections like cystic fibrosis, wound infection, urinary tract infection, ear infections, endocarditis, nosocomial infections and bacteremia, etc. Furthermore, due to their phenotypic variations; identification maybe sometimes harder and consequently which delays their early diagnosis and treatment in infected patients.  Hence, a quick and accurate method for the identification of P. aeruginosa would be more helpful in their early diagnosis. The present work was aimed to use species-specific primer for the rapid and precise identification of P. aeruginosa strains. Multiple drug-resistant strains were selected and their genetic variability was studied using ERIC-PCR. The results showed that out of ten P. aeruginosa isolates, eight were found to be unique and genetically diverse.


Keywords: Pseudomonas aeruginosa, ERIC-PCR, MDR. Wound infection, Pus sample.

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Kumar D, Sivakumar S, Sharmila P, Sivasubramani K. ERIC-PCR: A Molecular Typing Tool for Genotyping Multi Drug Resistant Pseudomonas aeruginosa Isolated from the Pus Samples. JDDT [Internet]. 13Nov.2019 [cited 18Jun.2021];9(4-s):1007-11. Available from: https://jddtonline.info/index.php/jddt/article/view/3712