ERIC-PCR: A Molecular Typing Tool for Genotyping Multi Drug Resistant Pseudomonas aeruginosa Isolated from the Pus Samples

Authors

  • Deepak Kumar Department of Microbiology, Faculty of Science, Annamalai University, Annamalainagar-608002, India
  • S Sivakumar Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India
  • P Sharmila Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India
  • K Sivasubramani Optimurz Bio-Science, Madras Institute of Magnetobiology, Anna Nagar, Chennai – 600040, India

Abstract

Pseudomonas aeruginosa is a typical and major human pathogen, it causes numerous risky opportunistic infections like cystic fibrosis, wound infection, urinary tract infection, ear infections, endocarditis, nosocomial infections and bacteremia, etc. Furthermore, due to their phenotypic variations; identification maybe sometimes harder and consequently which delays their early diagnosis and treatment in infected patients.  Hence, a quick and accurate method for the identification of P. aeruginosa would be more helpful in their early diagnosis. The present work was aimed to use species-specific primer for the rapid and precise identification of P. aeruginosa strains. Multiple drug-resistant strains were selected and their genetic variability was studied using ERIC-PCR. The results showed that out of ten P. aeruginosa isolates, eight were found to be unique and genetically diverse.

Keywords: Pseudomonas aeruginosa, ERIC-PCR, MDR. Wound infection, Pus sample.

DOI

https://doi.org/10.22270/jddt.v9i6.3712

References

Dworkin M, Falkow S, Schleifer K-H, Rosenberg E, Stackebrandt E, editors. The Prokaryotes A Handbook on the Biology of Bacteria. New York: Springer Science Business Media, Inc.; 2006. p. 704-713.

Driscoll, J.A., Brody, S.L., and Kollef, M.H. (2007) The epidemiology, pathogenesis and treatment of Pseudomonas aeruginosa infections. Drugs 67: 351-368.

Brooks, G. F.; Butel, J. S.; Carroll, K. C. and Morse, S. A. (2007). Jawetz, Melnick and Adelbergُ s Medical Microbiology. 24th ed. The McGraw-Hill Companies, Inc. USA.

Tortoli, E. 2003. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin. Microbiol. Rev. 16:319-354.

Woese, C. R. 1987. Bacterial evolution. Microbiol. Rev. 51:221-271.

Bottger, E. C. 1989. Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol. Lett. 65:171-176.

Potron A, Poirel L, Nordmann P. Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacterbaumannii: mechanisms and epidemiology. Int J Antimicrob Agents. 2015; 45: 568–585. https://doi.org/10.1016/j.ijantimicag. 2015. 03.001PMID: 25857949.

Bae IK, Suh B, Jeong SH, Wang KK, Kim YR, Yong D, et al. Molecular epidemiology of Pseudomonas aeruginosa clinical isolates from Korea producing β-lactamases with extended-spectrum activity. DiagnMicrobiol Infect Dis. 2014; 79: 373–377. https://doi.org/10.1016/j.diagmicrobio.2014.03.007 PMID:24792837

Poirel L, Naas T, Nordmann P. Diversity, epidemiology, and genetics of class D β-lactamases. Antimicrob Agents Chemother. 2010; 54: 24–38. https://doi.org/10.1128/AAC.01512-08 PMID: 19721065.

Tsutsui A, Suzuki S, Yamane K, Matsui M, Konda T and Marui E. Genotypes and infection sites in an outbreak of multidrug resistant Pseudomonas aeruginosa. J Hosp Infect. 2011; 78:317-22. PMID: 21689862.

Chemother. 2016;60: 3856–3861. https://doi.org/10.1128/AAC.03113-15 PMID: 27067331.

Hocquet D, Talon D. Molecular epidemiology of multidrug-resistant Pseudomonas aeruginosa in a French university hospital. J Hosp Infect. 2010; 76:316-9. PMID: 20692070.

Syrmis MW, O’Carroll MR, Sloots TP, Coulter C, Wainwright CE, Bell SC, Nissen MD. Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays. J Med Microbiol. 2004; 53:1089–96. PMID: 15496385.

V. B. D. Skerman, A Guide to the Identification of the Genera of Bacteria with methods and digests of generic characteristics. IX u. 217 Seiten, 3 Abb., 3 Tab., 31 Tafeln. Baltimore 2, Maryland, USA 1959:

Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. | |. Created: Tuesday, 08 December 2009. Author. • Jan Hudzicki. Information.

Elizabeth B Hirsch and Vincent H Tam ... While the overall proportion of infections caused by P. aeruginosa has ... in addition to its ability to acquire and harbor diverse

Yu-Ping Hong, Ying-Shu Liao, You-Wun Wang, Yueh-Hua Tu, ... The MDR strains of SL3_2 first appeared in 2015 and were resistant or of Salmonella enterica Serovar Anatum Clone, Taiwan, 2015

De-Vos, D., A. Lim, Jr., J. P. Pirnay, M. Struelens, C. Vandenvelde, L. Duinslaeger, A. Vanderkelen, and P. Cornelis. 1997. Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J. Clin. Microbiol. 35:1295–1299.

Lim, W.S., Respiratory Medicine, Nottingham University Hospitals for endorsement in January 2009.

Kanj, M.D.S.S. and M.D.Z.A.Kanafani. Current concepts in antimicrobial therapy against resistant gram-negative organisms: extended-spectrum Lactamase–producing Enterobacteriaceae, carbapenem-Resistant Enterobacteriaceae, and Multidrug-Resistant Pseudomonas aeruginosa. Mayo ClinProc 2011; 86:250–9.

Perez F, Endimiani A, Hujer KM, Bonomo RA. The continuing challenge of ESBLs.CurrOpinPharmacol 2007;7:459–69.

Aljindan R, Alsamman K, Elhadi N. ERIC-PCR genotyping of Acinetobacterbau-mannii isolated from different clinical specimens. Saudi J Med Sci 2018; 6:13–7.

Ranjbar R, Tabatabaee A, Behzadi P, Kheiri R. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genotyping of Escherichia coli strains isolated from different animal stool specimens. Iran J Pathol 2017;12(1):25–34.

Spilker, T., Coenye, T., Vandamme, P., &LiPuma, J. J. (2004). PCR-Based Assay for Differentiation of Pseudomonas aeruginosa from Other Pseudomonas Species Recovered from Cystic Fibrosis Patients. Journal of Clinical Microbiology, 42(5), 2074–2079. https://doi.org/10.1128/JCM.42.5.2074-2079.2004.

Goudarzi H, Karimi F, AsadiAmoli F, Abedinyfar Z, Doustdar F, Mehrnejad F. Genetic fingerprinting and antimicrobial susceptibility profiles of Pseudomonas aeruginosa isolates from eye infections. Iranian J Clin Infect Dis. 2011, 6(1):41-6.

Dawson SL, Fry JC, Dancer BN. A comparative evaluation of five typing techniques for determining the diversity of fluorescent pseudomonads. J Microbiol Methods. 2002;50(1):9-22. PMID: 11943354.

Inacio HS, Bomfim MR, França RO, Farias LM, Carvalho MA, Serufo JC, Santos SG. Phenotypic and genotypic diversity of multidrug-resistant Pseudomonas aeruginosa Isolates from bloodstream infections recovered in the Hospitals of Belo Horizonte, Brazil. Chemotherapy. 2014, 60(1):54-62. PMID: 25402327.

Khosravi, A.D., H. Hoveizavi, A. Mohammadian, A. Farahani and A. Jenabi, 2016. Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR.ActaCirúrgicaBrasileira, 31(3): 206-211.

Published

13-11-2019
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How to Cite

1.
Kumar D, Sivakumar S, Sharmila P, Sivasubramani K. ERIC-PCR: A Molecular Typing Tool for Genotyping Multi Drug Resistant Pseudomonas aeruginosa Isolated from the Pus Samples. J. Drug Delivery Ther. [Internet]. 2019 Nov. 13 [cited 2025 Feb. 18];9(4-s):1007-11. Available from: https://jddtonline.info/index.php/jddt/article/view/3712

How to Cite

1.
Kumar D, Sivakumar S, Sharmila P, Sivasubramani K. ERIC-PCR: A Molecular Typing Tool for Genotyping Multi Drug Resistant Pseudomonas aeruginosa Isolated from the Pus Samples. J. Drug Delivery Ther. [Internet]. 2019 Nov. 13 [cited 2025 Feb. 18];9(4-s):1007-11. Available from: https://jddtonline.info/index.php/jddt/article/view/3712