Green Synthesis of Silver Nanoparticles using Aqueous Cranberry Fruit Extract and its Antibacterial Activity

Authors

  • Pratibha Jinesh Shah Department of Microbiology K.C. College, Churchgate, Mumbai, India
  • Ruchi Malik Department of Microbiology K.C. College, Churchgate, Mumbai, India

Abstract

Objective: Cranberry (Vaccinium macrocarpon) is popularly used in traditional folk medicine for treatment of microbial infections. The aim of the present study was to evaluate the antibacterial activity of aqueous cranberry fruit extract (ACE) against pathogenic cultures and its application in green synthesis of silver nanoparticles.

Methods: ACE was screened for its antibacterial activity by agar well diffusion assay. The minimum inhibitory concentration (MIC) was determined by broth macrodilution technique, and minimum bactericidal concentration (MBC) was quantified. ACE was used in the green synthesis of silver nanoparticles (AgNPs), which were characterized by an Ultraviolet–visible (UV-VIS) spectroscopy and Field emission gun-scanning electron microscopy (FEG-SEM) techniques. Agar well diffusion assay was used to evaluate the antibacterial activity of the AgNPs formed.

Results: The zone of inhibition (ZOI) for ACE was found to be in the range of 19 - 30.3 mm, for the concentration of 100% (v/v). The MIC values were in the range of 12.5% - 50% (v/v) and the values indicated that a concentration of 50% (v/v) ACE could inhibit 87.5% (7/8) test cultures. The formation of AgNPs was confirmed by UV-VIS spectroscopy and the surface-plasmon resonance peak was observed at 430 nm. The FEG-SEM analysis revealed that the most of AgNPs were spherical in shape and had 15-25 nm size range. All the test cultures were inhibited by the AgNPs and the average ZOI measured 25.7±5.6 mm.

Conclusion: Cranberry fruit extract has a potent antibacterial activity against pathogens and it can be applied in green synthesis of silver nanoparticles.

Keywords: Cranberry, MIC, MBC, silver nanoparticles, FEG-SEM.

DOI

https://doi.org/10.22270/jddt.v9i4-s.3705

Author Biographies

Pratibha Jinesh Shah, Department of Microbiology K.C. College, Churchgate, Mumbai, India

Department of Microbiology K.C. College, Churchgate, Mumbai, India

Ruchi Malik, Department of Microbiology K.C. College, Churchgate, Mumbai, India

Department of Microbiology K.C. College, Churchgate, Mumbai, India

References

Banerjee J, Narendhirakanan RT. Biosynthesis of silver nanoparticles from Syzygium cumini (L.) seed extract and evaluation of their in vitro antioxidant activities. Digest Journal of Nanomaterials and Biostructures. 2011; 6:961-968.

Ashour AA, Raafat D, El-Gowelli HM, El-Kamel AH. Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties. International Journal of Nanomedicine. 2015; 10:7207-7221.

Kumar B, Smita K, Cumbal L. Debut, A. Green synthesis of silver nanoparticles using Andean blackberry fruit extract. Saudi journal of biological sciences. 2017; 24(1), 45-50.

Blumberg JB, Camesano TA, Cassidy A, Kris-Etherton P, Howell A, Manach C et al. Cranberries and their bioactive constituents in human health. Advances in Nutrition. 2013; 4(6):618-632.

Stobnicka A, Jungfer E, Gniewosz, M. Composition and antibacterial properties of fresh cranberry (Vaccinium macrocarpon L.) juice. Postepy Fitoterapii. 2013; 2:85-89.

Punjabi K, Chitalia VK, Mukadam T, Sharma J, Maniar J, Vasave S, Vaidya S, Dikho Mao L. Evaluation of the antimicrobial activity of Gooseberry and Wild Apple fruit juices of the northeast region of India. Journal of Medicinal Herbs and Ethnomedicine. 2017; 3:1-7.

Veljić M, Tarbuk M, Marin P, Ćirić A, Soković M, Marin M. Antimicrobial Activity of Methanol Extracts of Mosses from Serbia. Pharmaceutical Biology. 2008; 46(12):871-875.

Shah, P., & Malik, R. Study of Antibacterial activity of Phyllanthus emblica and its role in Green Synthesis of Silver Nanoparticles. Journal of Drug Delivery and Therapeutics, 2019; 9(3):76-81.

CLSI, Methods for Determining Bactericidal Activity of Antimicrobial Agents. Approved Guideline, CLSI document M26-A. Clinical and Laboratory Standards Institute, 950 West Valley Roadn Suite 2500,Wayne, Pennsylvania 19087, USA, 1998.

Tyagi S, Kumar A, Tyagi PK. Comparative analysis of metal nanoparticles synthesized from Hibiscus rosa sinesis and their antibacterial activity estimation against nine pathogenic bacteria. Asian Journal of Pharmaceutical and Clinical Research. 2017; 10(5):323-329.

Balouiri, M.; Sadiki, M.; Ibnsouda, S.A. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical and Biomedical Analysis. 2016; 6:71–79 .

Ibrahim OMS, Sarhan SR, Hameed AA. In Vivo and in vitro antibacterial activities of cranberry extract against E. coli O157:H7 in urinary tract infected rats. Advances in animal and veterinary Sciences. 2015; 3(4):233-244.

Suwan T, Wanachantararak P, Khongkhunthian S, Okonogi S. Antioxidant activity and potential of Caesalpinia sappan aqueous extract on synthesis of silver nanoparticles. Drug Discoveries and Therapeutics. 2018; 12(5):259-266.

Published

11-11-2019
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How to Cite

1.
Shah PJ, Malik R. Green Synthesis of Silver Nanoparticles using Aqueous Cranberry Fruit Extract and its Antibacterial Activity. J. Drug Delivery Ther. [Internet]. 2019 Nov. 11 [cited 2025 Feb. 12];9(4-s):991-5. Available from: https://jddtonline.info/index.php/jddt/article/view/3705

How to Cite

1.
Shah PJ, Malik R. Green Synthesis of Silver Nanoparticles using Aqueous Cranberry Fruit Extract and its Antibacterial Activity. J. Drug Delivery Ther. [Internet]. 2019 Nov. 11 [cited 2025 Feb. 12];9(4-s):991-5. Available from: https://jddtonline.info/index.php/jddt/article/view/3705