Influence of Growth Hormone and Additives on In vitro Shoot Initiation from Apical and Axillary Meristems of Commiphora wightii
Commiphora wightii is an important plant species of immense medicinal value and pharmaceutically used for the treatment of various diseases. The present study was designed to develop an efficient protocol for micropropagation of Commiphora wightii from apical and nodal meristems and to study the influence of additives on the enhancement of bud breaking, number of shoot per explant and shoot length. MS (Murashige and Skoog, 1962) basal media supplemented with various concentrations of 6- Benzyl amino purine (BAP: 0.5-5.0 mg/l) alone or in combinations with kinetin (KN: 0.5-1.0 mg/l) and naphthalene acetic acid (NAA: 0.5-1.0 mg/l) and additives like ascorbic acid (AA), citric acid (CA), adenine sulphate (AS) and activated charcoal (AC). The highest shoot initiation was reported in T13 (MS + 4.0BAP + 1.0 NAA mg/l + Additives) i.e. 0.80 ± 0.07 with highest number (1.57 ± 0.15) of shoots per explants. Treatment T12 (MS + 3.0BAP + 1.0 NAA+1.0 KN mg/l + Additives) highest shoot length (1.13) was recorded followed by T13 (MS + 4.0BAP + 1.0 NAA mg/l Additives) and T14 (MS + 5.0BAP + 1.0 KN) having mean value 1.07 and 0.90 respectively. MS basal medium (control) devoid of growth regulators did not support the shoot induction. The shoot induction protocol developed in current study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant
Keywords: Commiphora wightii, 6- Benzyl amino purine, kinetin, naphthalene acetic acid, additives.
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