A comparative study on the antioxidant activity of Cassia sophera (L.) leaf and bark extracts

  • Senjuti Majumder Southeast University
  • Ishrat Jahan Afia

Abstract

Background: Cassia sophera (L.), or kasundi, is an important plant in traditional medicinal system belonging to family caesalpiniaceae. The leaves extract of this plant is known for various pharmacological activities including, anti-inflammatory, anti-rheumatic, purgative property etc.


Objectives: The objective of the present study was to evaluate and compare the antioxidant potential of methanolic leaf and stem bark extracts of C. sophera (L.).


Methods: We measured and compared antioxidant potential by using DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging, ferric reducing power antioxidant capacity and total antioxidant capacity assay;  and through determination  of  total  phenol  and  flavonoid  content determination. 


Results: Preliminary phytochemical  study  revealed  the  presence  of  alkaloid, glycoside, tannin, saponin  and  flavonoid  in  both  extracts. The extracts showed moderate antioxidant activity in a dose dependent manner. In DPPH radical scavenging assay, the IC50 value of the leaf and bark extracts was 204.44 µg/ml and 297.37 µg/ml, respectively, whereas IC50 value for the reference ascorbic acid was 19.08 µg/ml. Furthermore, both the extracts showed moderate antioxidant activity in Ferric reducing power and Total antioxidant capacity assay.  In  addition,  the bark  extract  exhibited  higher  amount  of  flavonoid and phenolic content  compared  to the leaf  extract,  which were expressed  as  Quercetin and Gallic  Acid equivalent respectively. Conclusion: Based on these findings, it can be concluded that both the extracts  possesses  equitable antioxidant potential of which methanolic stem bark extract of C. sophera is more promising one and possess higher antioxidant potential.


KEYWORDS: Cassia sophera, In-vitro, Antioxidant capacity, DPPH radical, IC50, Reducing power, Flavonoid.

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Author Biography

Ishrat Jahan Afia

Department of Pharmacy, Southeast University, Banani, Dhaka-1213,

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1.
Majumder S, Afia IJ. A comparative study on the antioxidant activity of Cassia sophera (L.) leaf and bark extracts. JDDT [Internet]. 15Aug.2019 [cited 18Jun.2021];9(4-s):177-81. Available from: https://jddtonline.info/index.php/jddt/article/view/3241