Antimicrobial activities of Chaetomorpha antennina dichloromethane extract against isolated urinary tract infection pathogens
During the treatment of infectious diseases, upon repeated administration of an antibiotic, most microbial pathogens generate resistance to that antibiotic. Multiple drug resistance is also a widespread problem. Thus, it is the need of the hour to identify alternative antibacterial drugs. Seaweeds are one of the major repositories of numerous pharmacologically active secondary metabolites. The goal of this investigation is to evaluate the antimicrobial activity of Chaetomorpha antennina dichloromethane (DCM) extract against isolated urinary tract infection pathogens from urinary tract infection (UTI) infected patients. Antimicrobial activity, minimum inhibitory concentrations (MIC), minimum bactericidal or candicidal concentration (MBC/MCC) and biofilm inhibition activity of C. antennina extract was observed against the 7 isolated microbial strains: Enterococcus avium, Enterobacter cloacae, Staphylococcus hominis, Staphylococcus epidermidis, Pseudomonas aeruginosa, Proteus mirabilis, and Escherichia coli and Candida albicans (yeast). All isolated strains from UTI infected patients were later sequenced, identified and submitted in PubMed. The crude DCM extract of C. antennina showed remarkable inhibitory effects ranging between 2mm and 9mm, and its activity was recorded in the following order of E. coli >E. avium> E. cloacae and S. hominis >S. epidermis > P. aeruginosa > P. mirabilis > C. albicans. The MIC ranged between 500 to 750µg/ml, and the MBC was recorded in the range of 750 to 1000µg/ml. The crude DCM extract of C. antennina was also observed to have biofilm inhibitory activity at different concentrations against isolated UTI pathogens. This investigation elucidates that C. antennina DCM extract possesses antimicrobial activity against isolated UTI pathogens.
Keywords: Antimicrobial activities, Chaetomorpha antennina, Minimum inhibitory concentrations and Urinary tract infection.
2. Flores-Mireles AL, Walker JN, Caparon M, Hultgren SJ. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nat Rev Microbiol. 2015; 13: 269–84.
3. Foxman B. The epidemiology of urinary tract infection. Nat Rev Urol.2010; 7: 653–60.
4. Sharma A, Chandraker S, Patel VK, Ramteke P. Antibacterial Activity of Medicinal Plants against Pathogens causing Complicated Urinary Tract Infections. Indian J Pharm Sci. 2009; 71: 136–139.
5. Khotimchenko SV, Vaskovsky VE and Titlyanova TV. Fatty acids of marine algae from the Pacific coast of North California. Botanica Marina. 2002; 45 (1); 17-22.
6. Vijayan SR, Santhiyagu P, Ramasamy R, Arivalagan P, Kumar G, Ethiraj K. and Ramaswamy BR. Seaweeds: A resource for marine bionanotechnology. Enzyme and microbial technology, 2016: 95, pp.45-57.
7. El Shafay SM, Ali SS and El-Sheekh MM. Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria. The Egyptian Journal of Aquatic Research, 2016; 42 (1); 65-74.
8. Choudhury S, Sree A, Mukherjee SC, Pattnaik P, Bapuji M. In vitro antibacterial activity of extracts of selected marine algae and mangroves against fish pathogens. Asian Fisheries Science, 2005; 18(3/4): 285-294.
9. Ravikumar S, Ramanathan G, Inbaneson SJ, Ramu A. Antiplasmodial activity of two marine polyherbal preparations from Chaetomorpha antennina and Aegiceras corniculatum against Plasmodium falciparum. Parasitology Research. 2011; 108 (1); 107-113.
10. Palanisamy S, Sellappa S. Antioxidant and antibacterial properties of methanolic extract of green seaweed Chaetomorpha linum from Gulf of Mannar: Southeast coast of India. Jundishapur Journal of Microbiology, 2012; 12(2):411-415.
11. Maheswari MU, Reena A and Sivaraj C. GC-MS analysis, antioxidant and antibacterial activity of the brown algae, Padina tetrastromatica. International Journal of Pharmaceutical Sciences and Research, 2018; 9(1): 298-304.
12. Edeoga HO, Okwu DE and Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology, 2005; 4(7): 685-688.
13. Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta medica, 1998; 64(08): 711-713..
14. Sharma D, Narasimhan B, Kumar P, Judge V, Narang R, De Clercq E and Balzarini J. Synthesis, antimicrobial and antiviral evaluation of substituted imidazole derivatives. European journal of medicinal chemistry, 2009; 44(6): 2347-2353.
15. Prakash S, Ahila NK, Ravikumar S and Kannapiran E. Phenotypic and genotypic probing of biofertilizing halotolerant Azospirillum spp. and Bacillus spp. Middle-East Journal of Scientific Research, 2013; 15(1); 128-133.
16. Clinical and Laboratory Standards Institute (CLSI). Document M100-S24. Wayne, Pa, USA: CLSI; 2014. Performance standards for antimicrobial susceptibility testing; twenty-forth informational supplement.
17. Bazargani MM and Rohloff J. Antibiofilm activity of essential oils and plant extracts against Staphylococcus aureus and Escherichia coli biofilms. Food Control, 2016; 61: 156-164.
18. Manikandan SS, Ganesapandian, M. Singh, N. Sangeetha and A.K. Kumaraguru. Antimicrobial activity of seaweeds against multi drug resistant strains. Int. J. Pharmacol. 2011; 7: 522-526.
19. Sivakumar SM, Safhi SM. Isolation and screening of bioactive principle from Chaetomorpha antennina against certain bacterial strains. Saudi Pharmaceutical Journal, 2013; 21, 119–121.
20. Subathraa K and Poonguzhali TV. Effect of different extracts of Chaetomorpha antennina and their phytochemical screening. Int J Curr Sci, 2013; 6: 35-39.
21. Abhishek D, Jyoti P, Savan D and Sumitra C. Pharmacognostic standardization of chaetomorpha antennina and Ulva lactuca, green seaweeds from Gujarat coast Journal of Pharmacognosy and Phytochemistry, 2018: 7(2); 3863-3870.
22. Lima-Filho JVM, Carvalho AF, Freitas SM and Melo VM. Antibacterial activity of extracts of six macroalgae from the northeastern Brazilian coast. Brazilian Journal of Microbiology, 2002; 33(4); 311-314.
23. Latha PD and Hemalatha KPJ. Antimicrobial activity of the chloroform extracts of the chlorophycean seaweeds Enteromorpha comressa and Chaetomorpha antennina. Int. Res. J. Microbiol. 2011; 2(8): 249-252.
24. Phillips I, Andrews JM, Bridson E, Cooke EM, Spencer RC, Holt HA, Wise R, Bint J, Brown D. FJ,Greenwood, D, King A and Williams RJ. A Guide to Sensitivity Testing. Report of the Working Party on Antibiotic Sensitivity Testing of the British Society for Antimicrobial Chemotherapy, 1991; 27: 1-50.
25. Erturk Omer and Taş Beyhan. Antibacterial and Antifungal Effects of Some Marine Algae. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17: S121-S124.
26. Namasivayam SKR, Beninton B, Christo B, Karthigai SM, Arun Muthu Kumar K, Deepak K. Anti-biofilm effect of biogenic silver nanoparticles coated medical devices against biofilm of clinical isolate of Staphylococcus aureus. Global Journal of Medical Research. 2013; 13(3):1–7.
27. Murugan A, Meera. RL, Raja P, Senthil KA. Antibacterial activity of the seaweed Chaetomorpha antennina and its epiphytic bacteria against biofilm and human pathogenic bacteria. Seaweed Research and Utilisation, 2007: 29 (1-2); 67-74.
28. Mubarak Z, Humaira A, Gani B.A and Muchlisin ZA. Preliminary study on the inhibitory effect of seaweed Gracilaria verrucosa extract on biofilm formation of Candida albicans cultured from the saliva of a smoker. F1000Research. 2018; 7:684.
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