Fortification role of Curcumin against renal and testicular toxicity of synthetic food dye brilliant blue in rats
Abstract
Increasing awareness has been lately paid to the toxicity of synthetic additives used in food. The main aim of this study was to survey the renal and testicular toxicity of synthetic food dye brilliant blue. Administration of the BB changed body and relative organs weights, serum creatinine, urea (BUN), uric acid and serum FSH, LH, testosterone levels. This study proved that BB induced oxidative damage as manifested by significant increase in Lipid peroxidation with disorganization in the activity of glutathione peroxidase, protein carbonyl and reactive oxygen species content. Histopathological changes include: infiltration and vacuolation in kidney. In addition; degeneration and necrosis of spermatogoneal cells lining seminiferous tubules in testis. Furthermore; BB induced appotosis via activation of casp-3. Administration of curcumin with BB attenuated the cytotoxic effects of brilliant blue on kidney and testis tissues and reducing apoptotic cell death as well as improved the redox status of kidney and testis.
Keywords: Brilliant blue dye, Curcumin, Kidney, Testis, Histopathology, Rats.
DOI
https://doi.org/10.22270/jddt.v9i2.2448References
- de Boer L. Biotechnological production of colorants. Adv. Biochem. Eng.Biotechnol; 2014; 143:51e89.
- Newsome AG, Culver CA and van Breemen, RB. Nature's palette: the search for natural blue colorants. J Agric Food Chem; 2014; 62:6498e6511.
- Dixit S, Purshottam SK, Khanna SK and Das M. Usage pattern of synthetic food colours in different states of India and exposure assessment through commodities preferentially consumed by children. Food Addit Contam Part A Chem Anal Control Expo Risk Assess; 2011; 28:996–1005.
- Ashida H, Hashimoto T, Tsuji S, Kanazawa K and Danno G, Synergistic effects of food colors on the toxicity of 3-amino-1,4-dimethyl-5H pyrido-indole in primary cultured rat hepatocytes. Journal of Nutritional Science and Vitaminology; 2000; 46(3):130–136.
- Moutinho ILD, Bertges LC and Assis RVC, Prolonged use of the food dye tartrazine and its effects on the gastric mucosa of Wistar rats. Brazilian Journal of Biology; 2007; 67(1):141–145.
- Shousha MA, Sakr AA, Hammam MA and Abdel-Moein NM. Effect of synthetic banana food additives on energy and nucleic acids metabolism in brain, liver and kidney tissues of albino rats. Egyptian Journal of Applied Sciences; 1992; 7(7):45–55.
- El-Wahab HM and El-Deen Moram GS. Toxic effects of some synthetic food colorants and/or flavor additives on male rats. Toxicology and industrial health; 2013; 29(2):224-32.
- Iovdijová A and Bencko V (2010). Potential risk of exposure to selected xenobiotic residues and their fate in the food chain-Part. I: Classification of xenobiotics. Annals of Agricultural and Environmental Medicine; 17(2):183-92.
- Vrzal R, Ulrichová J and Dvorak Z. Aromatic hydrocarbon receptor status in the metabolism of xenobiotics under normal and pathophysiological conditions. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub; 2004; 148(1):3-10.
- Onyema OO, Farombi EO, Emerole GO, Ukoha AI and Onyeze GO. Effect of vitamin E on monosodium glutamate induced hepatotoxicity and oxidative stress in rats. Indian Journal of Biochemistry & Biophysics; 2006; 43:20-24.
- Trujillo J, Chirino YI, Molina-Jijón E, Andérica-Romero AC, Tapia E and Pedraza-Chaverrí J. Renoprotective effect of the antioxidant curcumin: Recent findings. Redox Biol; 2013; 1(1):448-56.
- Koeberle A and Werz O. Multi-target approach for natural products in inflammation. Drug Discov Today; 2014; 19:1871–1882.
- Institute of Laboratory Animal Resources. Guide for the care and use of laboratory animals, 8th edition. Washington, DC: National Academy Press; 1996.
- Walton K, Walker R, Van De Sandt JJM and Castell JV. The Application of In Vitro in the Derivation of the Acceptable Daily Intake of Food Additives. Food Chem Toxicol; 1999; 37:1175-1197.
- Farghaly HS and Hussein MA. Protective effect of curcumin against paracetamol-induced liver damage. Australian Journal of Basic and Applied Sciences; 2010; 4(9):4266-4274.
- Wybenga DR, Glorgio JD and Pileggi VJ. Manual and automated methods for urea nitrogen measurement in whole serum. Clin Chem; 1971; 17:891–895.
- Henry RJ (1974). Determination of serum creatinine. Clinical Chemistry: Principles and Techniques. 2nd ed. Harper and Row Publishers, New York.
- Young DS (2001). Effects of disease on clinical lab. testes. 4th ed., AACC.
- Vrablic AS, Albright CD, Craciunescu CN, Salganik RI and Zeisel SH. Altered mitochondrial function and overgeneration of reactive oxygen species precede the induction of apoptosis by 1-O-octadecyl-2-methyl-rac-glycero-3-phosphocholine in p53-defective hepatocytes. The FASEB Journal; 2001; 15(10):1739-1744.
- Uchiyama M and Mihara M. Determination of malonaldehyde precursor in tissue by thiobarbituric acid test. Anal Biochem; 1978 ; (86):271-278.
- Smerjac SM and Bizzozero OA. Cytoskeletal protein carbonylation and degradation in experimental autoimmune encephalomyelitis. Journal of neurochemistry; 2008; 105(3):763-772.
- Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidases. Free Radic Biol Med; 1999; 27:951–965.
- Banchroft JD, Stevens A and Turner DR. Theory and practice of histological techniques, 4th edition. New York: Churchill Livingstone. 1996.
- Chatterjea MN and Shinde R (2002). Text Book of Medical Biochemistry, fifth ed. Jaypee Brothers, Medical Publishers Ltd., New Delhi, p. 317.
- Mishra S and Palanivelu K. The effect of curcumin (turmeric) on Alzheimer's disease: An overview. Ann Indian Acad Neurol; 2008; 11:13–19.
- Nair HB Sung B, Yadav VR, Kannappan R, Chaturvedi MM and Aggarwal BB. Delivery of antiinflammatory nutraceuticals by nanoparticles for the prevention and treatment of cancer. Biochem Pharmacol; 2010; 80:1833–1843.
- Helal EG, Zaahkouk SA and Mekkawy HA. Effect of some food colorants (synthetic and natural products) of young albino rats. I–Liver and kidney functions. The Egyptian Journal of Hospital Medicine; 2000; 1:103-113.
- Ashour AA and Abdelaziz I. Role of fast green on the blood of rats and the therapeutic action of vitamins C or E. Int J Integr Biol; 2009; 6(1):6-11.
- Yousef MI, El-Demerdash FM and Radwan FM. Sodium arsenite induced biochemical perturbations in rats: ameliorating effect of curcumin. Food and Chemical Toxicology; 2008; 46(11):3506-3511.
- Jagadeesh MC, Sreepriya M, Bali G and Manjulakumari D. Biochemical studies on the effect of curcumin and embelin during N-nitrosodiethylamine/phenobarbital induced-hepatocarcinogenesis in wistar rats. African Journal of Biotechnology; 2009; 8(18).
- Himri I, Bellahcen S, Souna F, Belmekki F, Aziz M, Bnouham M, Zoheir J, Berkia Z, Mekhfi H and Saalaoui E. A 90-day oral toxicity study of tartrazine, a synthetic food dye, in wistar rats. Group; 2011; 300:00.
- El-Tohamy MM. The mechanisms by which oxidative stress and free radical damage produces male infertility. Life Sci J; 2012; 9(1):674-688.
- Kaneto H, Katakami N, Matsuhisa M and Matsuoka TA. Role of reactive oxygen species in the progression of type 2 diabetes and atherosclerosis. Mediators of inflammation. 2010.
- Amin KA, Hameid II HA and Elsttar AA. Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food and Chemical Toxicology; 2010; 48(10):2994-2999.
- Mahmoud NH. Toxic effects of the synthetic food dye brilliant blue on liver, kidney and testes functions in rats. J Egypt Soc Toxicol; 2006; 34:77-84.
- Carocho M and Ferreira IC. A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food and chemical toxicology; 2013; 51:15-25.
- Nanditha BR, Jena BS and Prabhasankar P. Influence of natural antioxidants and their carry-through property in biscuit processing. Journal of the Science of Food and Agriculture; 2009; 89(2):288-298.
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