Assessment of hemolytic effect of Cassia flower extracts on human RBCs

  • Shital S. Phuse Department of Biochemistry Shri Shivaji College of Arts, Commerce and Science, Akola 444003 (M.S)
  • Zia. H. Khan Department of Biochemistry, Shri Shivaji College of Arts, Commerce and Science, Akola, Maharashtra

Abstract

RBC membrane can be affected by consumption of bioactive compounds from herbs and medicinal plants. This study aimed to assess hemolytic effect of crude ethyl acetate and acetone extract from Cassia glauca flowers. Both the extracts of Cassia flowers were prepared, using Soxhlet apparatus. RBCs were washed with phosphate buffered saline and resuspended in 0.9% normal saline. These RBCs were added to different concentrations of the extracts and then incubated. After centrifugation, absorbance of the supernatant was determined by UV spectrophotometer at 540 nm. The present work shows that the fractions exhibited anti-hemolytic potential as extracts of Cassia flower showed very less percent of hemolysis when compared to standard quercetin. IC50values were found to be 23.77μg/ml for (CF EA) and 12.50μg/ml for Cassia flower in acetone(CF A)against standard which was found to be 41.75μg/ml. Extracts of Cassia flower exhibited very low hemolytic activity. Hence, it can be considered as safe to human RBCs.  In future recommend further in vitro and in vivo studies to evaluate the clinical efficacy of Cassia glauca extracts for treated several diseases.


Keyword: Extract, Acetone, Ethyl acetate, Cassia glauca flowers, Hemolytic effect, RBCs

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Author Biography

Zia. H. Khan, Department of Biochemistry, Shri Shivaji College of Arts, Commerce and Science, Akola, Maharashtra

Department of Biochemistry, Shri Shivaji College of Arts, Commerce and Science, Akola, Maharashtra

References

1. Kumar G, Karthik L, Bhaskara Rao KV. Hemolytic activity of Indian medicinal plants towards human erythrocytes: and in vitro study, Appl. Botany, 2011; 40:5534-5537.
2. Zohra M, Fawzia A, Hemolytic activity of different herbal extracts used in Algeria. International Journal of Pharma Sciences and Research, 2014; 5(8):495-500.
3. Noudeh GD, Sharififar F, Khatib M, Behravan E, Afzadi MA. Study of aqueous extract of three medicinal plants on cell membrane–permeabilizing and their surface properties. African Journal of Biotechnology, 2009; 9(1):10-116.
4. Rajeshwari CU, Shobha RI, Andallu B, Antihemolytic activity of various fractions of methanolic extract of coriander (Coriandrum sativumL.) leaves and seeds: A comparative study. Pakistan Journal of Food Sciences 2012; 22(1):1-6.
5. Shabbir M et al. Assessment of phytochemicals, antioxidant, anti-lipid peroxidation and anti-hemolytic activity of extract and various fractions of Maytenusroy leanus leaves. BMC Complementary and Alternative Medicine, 2013, 13:143.
6. The phinlap C, Pangjit K, Suttajit M, Sri Chairatanakool S. Anti-oxidant properties and anti-hemolytic activity of Psidiumguajava, Pandanousodorus and Rhinacanthusnasutus. Journal of Medicinal Plants Research, 2013; 7(27):2001-2009.
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Phuse S, Khan ZH. Assessment of hemolytic effect of Cassia flower extracts on human RBCs. JDDT [Internet]. 15Dec.2018 [cited 18May2024];8(6-s):18-0. Available from: https://jddtonline.info/index.php/jddt/article/view/2169