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Zūfā (Hyssopus officinalis L.): Unani Temperament, Traditional Therapeutic Uses, Phytochemistry, Pharmacological Actions, and Clinical  Potential: A Comprehensive Review

Mohd. Uzair Beg¹*, Amreen Zehra¹, Murad Husain², Md Akhter Hussain Jamali¹, M. A. Waheed¹

¹ Research Officer (Unani), Regional Research Institute of Unani Medicine (RRIUM), Bhadrak, Odisha, India.

² Research Associate (Unani), Regional Research Institute of Unani Medicine (RRIUM), Bhadrak, Odisha, India.

1. Introduction

Medicinal herbs have long been recognized for their ability to treat and prevent a wide range of health conditions, with records of their use dating back to ancient civilizations. In recent years, both developing and developed nations have shown increasing interest in medicinal plants and herbal preparations, largely because of their proven effectiveness and relatively favourable safety profiles. Traditional herbal medicine continues to serve as the foundation of primary healthcare for roughly 75% of the global population, particularly in less developed and developing regions. Among the various forms of traditional medicine, herbal therapies remain the most widely practiced and continue to experience strong worldwide demand. Moreover, medicinal plants supply approximately 80% of the raw materials required for the manufacture of pharmaceutical products.¹ Hyssopus officinalis L. (Family: Lamiaceae) is a valuable aromatic and medicinal perennial herb or subshrub renowned for its diverse therapeutic benefits. It is widely used in modern herbal medicine and also serves as a spice and flavouring agent in the food industry.² The genus Hyssopus comprises several species that are widely cultivated across Asia, Europe, and the temperate regions of the Americas for culinary, medicinal, and commercial importance.³ In India, H. officinalis is distributed in the Western Himalayan region, from Kashmir to Kumaon, at elevations ranging from 8,000 to 11,000 ft. It naturally grows on dry, rocky slopes, banks, and ruins, attaining a height of approximately 50–120 cm.⁴ Traditionally, H. officinalis has been employed in various systems of medicine for the management of numerous ailments. However, despite its long-standing medicinal use, many of its therapeutic claims remain inadequately supported by rigorous scientific evidence. Consequently, a comprehensive synthesis of the available literature on its phytochemical constituents, traditional and ethnomedicinal uses, and pharmacological activities reported in recent scientific studies is warranted to critically evaluate its therapeutic potential and identify future research directions.³

According to Pharmacographia Indica, Zūfā-i-Yābis is a small woody herb, approximately 6–8 inches tall, with a four-angled purplish stem, numerous oblong flower spikes, and pale-brown, three-angled seeds. The commercial drug is usually found in fragmented form with a pleasant sweet-hay aroma and a bitter taste. Traditional Unani authorities describe it as a stimulant, anthelmintic, and deobstruent. Although it has generally been attributed to Hyssopus officinalis, its spike-like inflorescence suggests that the commercial Persian drug may not exactly correspond to this species. ⁶

2. Morphology

Macroscopic examination: The leaves are opposite, dark green, glossy, entire, and lanceolate to oblong, measuring 2–4 cm in length and 0.5–1.0 cm wide, with an obtuse to acuminate apex. The plant has a strongly branched, multi-headed taproot and erect to decumbent, woody, highly branched stems reaching 0.5–0.7 m in height. The inflorescence is a 20–25 cm long terminal false spike composed of 4–10-flowered pseudoverticillasters. Flowers are pedicellate, complete, and hypogynous. The calyx is 15-nerved and five-toothed, while the corolla is bilabiate. There are four exserted, divergent stamens, with the lower pair longer than the upper; the anther cells are linear and spreading, and the style bears two subequal, subulate lobes. ^2,8 

Microscopy: The transverse section (T.S.) of the pedicel shows a solid central stele surrounded by a single-layered epidermis of rectangular, cuticularized cells and a multi-layered parenchymatous cortex. The stele is enclosed by a sclerenchymatous pericycle, followed by a continuous phloem, while the xylem consists of vessels, tracheids, fibres, and xylem parenchyma. Calcium oxalate crystals are present in the cortical cells, and characteristic uniseriate, unicellular trichomes occur on the epidermis. The T.S. of the sepal comprises upper and lower epidermis enclosing 3-4 layers of parenchymatous mesophyll with intercellular spaces, 4-5 vascular bundles, and calcium oxalate crystals. The petal has a similar structure but with smaller cells and 3-4 vascular bundles. The anther is tetrasporangiate with four pollen sacs, a single-layered epidermis, and an endothecium showing characteristic lignified radial and tangential wall thickenings. ⁸

Powder Microscopy: The powder is brownish-green, odourless, and contains fragments of the pedicel, calyx, corolla, anther epidermis, endothecium, pollen grains, tracheids, xylem parenchyma, and characteristic uniseriate, unicellular trichomes. These features serve as reliable diagnostic markers for the authentication of the crude drug.  ⁸

The genus Hyssopus is naturally distributed across Europe, Asia, and North Africa. Additionally, introduced species have been observed in North America. ⁹

Parts Used: Leaves, Roots, flowers, Whole Plants. ^{8,10, 11} 

Temperament (Mizāj): Hot 3° and dry 3° ^8,10

Pharmacological Actions (Af‘āl): Mulattif: Attenuant; softens and dilutes thick humours, Munaffith-i-Balgham : Expectorant; facilitates the expulsion of phlegm from the respiratory tract. Muhallil-i-Waram : Anti-inflammatory; resolves inflammatory swellings. Kāsir-i-Riyāḥ : Carminative; disperses and relieves flatulence. Dāfiʿ-i-Dīdān-i-Amʿā: Anthelmintic; expels intestinal worms. Muʿarriq: Diaphoretic; promotes perspiration. ⁸

 Munaffith-i-Balgham: Expectorant; facilitates the expulsion of phlegm from the respiratory tract. Muhallil-i-Riyāḥ: Carminative; disperses thick and accumulated flatulence.

Qātil-i-Dīdān: Anthelmintic; expels intestinal worms. Muhallil-i-Awrām: Anti-inflammatory; resolves inflammatory swellings. Muhallil-i-Dam-i-Ghalīẓ: Resolves thick or congested blood.  Muqawwī-i-Aʿṣāb: Nervine tonic; strengthens the nerves. Mufattiḥ-i-Sud: Deobstruent; helps remove bodily obstructions. ^[12,13]

3. Classical Unani Therapeutic Uses (Mawāqiʿ-i-Istiʿmāl)

According to classical Unani literature, Zūfā (Hyssopus officinalis L.) is valued for its diverse therapeutic applications. Oral administration is believed to improve complexion, while topical application helps reduce facial blemishes and discoloration. It is particularly recommended for chest and lung ailments, including asthma, chronic cough, and orthopneic breathing. A decoction prepared with fig and honey is traditionally used to relieve respiratory complaints and resolve hard swellings. As a gargle, it is considered beneficial in inflammatory conditions of the throat associated with suffocation. Beyond respiratory uses, Zūfā is also described as a remedy for hard swellings, especially when taken with wine. A decoction with vinegar is traditionally employed to alleviate toothache, while the vapour of its decoction, particularly when combined with its stalks and figs, is directed into the ear to relieve tinnitus. For ocular conditions, the decocted herb is applied as a poultice to reduce ecchymosis and sub palpebral blood spots, reflecting its broad therapeutic significance in classical Unani medicine.¹⁰ 

Zoʿf-i-Ishtihā: Loss of appetite. Nafkh-i-Shikam: Abdominal flatulence and distension.  Suʿāl: Cough.  Nazla: Coryza/catarrh (common cold with nasal discharge). Ḍīq al-Nafas: Breathlessness/asthma. Khushūnat-i-Halq: Roughness or irritation of the throat. ⁸

 Suʿāl Muzmin: Chronic cough.  Ḍīq al-Nafas: Breathlessness and respiratory congestion. Wajaʿ al-Ṣadr: Chest pain. Dhāt al-Janb: Pleuritic pain and pleurisy.  Qūlanj: Colitis and abdominal colic associated with flatulence. Irq al-Nisā: Sciatica.  Fālij: Paralysis. Dīdān-i-Amʿā: Intestinal worm infestation.  ^12,13   

4. Phytochemical constituents

The chemical composition of Hyssopus officinalis is highly diverse and varies based on its geographic origin and environmental growing conditions. Its primary components include essential oils, flavonoids, resins, mineral salts, and both oleanolic and ursolic acids, tannins, and organic acids.  ¹⁴ Various classes of chemicals were isolated from Hyssopus officinalis L. flower tips contain ursolic acid and the glucoside diosmin. The main components of the essential oil are bicyclic monoterpenes (L-pinocamphene, cis-pinocamphone, pinocarvone, β-pinene), depending on the chemotype of the plant. The major components of H. officinalis include apigenin, quercetin, diosmin, luteolin and their glycosides, as well as chlorogenic, protocatechuic, ferulic, syringic, p-hydroxybenzoic, caffeic and other acids. ⁹ Subsp. angustifolius revealed that the aqueous leaf extract contained the highest total phenolic and flavonoid contents, measuring 1.3% (gallic acid equivalents, GAE) and 4.7%, respectively. Proanthocyanidins were detected in high concentrations in the aqueous, chloroform, and hexane leaf extracts, with the chloroform extract exhibiting the highest level (10,250 mg/L). High-performance liquid chromatography (HPLC) analysis further confirmed the presence of major antioxidant phenolic compounds, including chlorogenic acid (166.21 µg/g) and caffeic acid (111.09 µg/g) in the methanolic leaf extract, highlighting the plant's rich antioxidant phytochemical profile. ^[3,15] The major phytoconstituents of Hyssopus officinalis include a diverse range of polyphenolic compounds, predominantly flavonoids such as luteolin, diosmin, quercetin, apigenin, and their glycosides, together with phenolic acids including caffeic, p-hydroxybenzoic, syringic, ferulic, protocatechuic, and chlorogenic acids. In addition, the essential oil obtained from the aerial parts is rich in terpenoids, with β-pinene, isopinocamphone, and Pino camphone identified as the principal volatile constituents. ^3,2 

5. Pharmacological Properties

Hyssopus officinalis L. exhibits a broad spectrum of pharmacological activities that support both its long-standing traditional use and its emerging therapeutic potential. Traditionally, it has been employed as an expectorant, antispasmodic, digestive aid, antifungal, and antimicrobial herb for the management of respiratory and gastrointestinal disorders. Its aerial parts are widely employed for respiratory ailments, including asthma, bronchitis, and cough. It  has also been used to treat rheumatic pain, bruises, wounds, blood pressure fluctuations, and neurological disorders such as anxiety and depression. ^{16, 17,18} The ethanolic extract of Hyssopus officinalis has demonstrated gastroprotective, anticoagulant, and antioxidant activities. Its gastroprotective effect is attributed to enhanced adhesion of the gastric mucosal barrier, while its antioxidant action reduces free radical generation, thereby helping to protect tissues from oxidative damage. ^16,19

Modern pharmacological studies have substantiated many of these traditional claims, demonstrating significant antioxidant, antimicrobial, anti-inflammatory, and smooth muscle-relaxant activities. ^14,20  The ethyl acetate fractions of its leaves and stems exhibit potent free-radical scavenging activity in DPPH and ABTS assays, along with strong ferric-reducing antioxidant power, effects largely attributed to its rich content of phenolic compounds and flavonoids, including quercetin, hesperidin, and esculin. These bioactive constituents also contribute to its ability to suppress cough, combat microbial infections, reduce inflammation, and promote gastrointestinal health by relaxing the smooth muscles of the digestive tract and alleviating painful spasms, thereby supporting its therapeutic applications in both respiratory and digestive ailments. ^14,21 Its also exhibits moderate antioxidant activity and antimicrobial effects against both Gram-positive and Gram-negative bacteria. In vitro studies have also demonstrated pronounced antifungal, insecticidal, and antiviral properties. Animal model research has revealed muscle-relaxant and antiplatelet effects. ⁹

Xanthine Oxidase (XO) Inhibitory Activity

Microwave-assisted extracts (MAE), selected for their high phenolic content and antioxidant activity, exhibited notable xanthine oxidase (XO) inhibitory effects, with inhibition ranging from 50–88%, compared with 90% for allopurinol. Among the tested Unani medicinal plants, Hyssopus officinalis showed the highest inhibition (88%), followed by Portulaca oleracea (85%), Origanum vulgare (82%), and Rubia cordifolia (80%), whereas Cichorium intybus (58%) and Achyranthes aspera (50%) showed comparatively lower activity. These findings suggest that H. officinalis is a promising natural XO inhibitor and may have potential in managing hyperuricemia and gout. Given the adverse effects associated with allopurinol, plant-derived XO inhibitors warrant further investigation as safer therapeutic alternatives. ²²  

Superoxide dismutase (SOD) Inhibitory Activity

is a highly thermostable antioxidant enzyme and a key indicator of cellular defence against oxidative stress, ranking among the most heat-stable enzymes (after ascorbate peroxidase) involved in reducing reactive oxygen species. Most plant extracts inhibited SOD activity (38.5–70.2%), with effectiveness ranked by extraction method as microwave-assisted extraction (MAE) > ultrasound-assisted extraction (UAE) > Soxhlet extraction (SE) > maceration (MC). These results were compared against vitamin C (75.2% inhibition) as the standard. The highest inhibition (>60%) was observed for Hyssopus officinalis (70.2%), Origanum vulgare (65.5%), Portulaca oleracea (63.3%), and Rubia cordifolia (60.1%). ^22,23

Antimicrobial, Antifungal, and Antiviral Activity

The essential oil of Hyssopus officinalis exhibits broad-spectrum antimicrobial activity, largely attributed to bioactive constituents such as β-pinene, pinocamphone, isopinocamphone, pinocarvone, terpinen-4-ol, carvacrol, and p-cymene. It has demonstrated inhibitory effects against several pathogenic bacteria, including Staphylococcus aureus, Escherichia coli, Bacillus cereus, Proteus hauseri, Listeria monocytogenes, L. ivanovii, Salmonella enteritidis, and Enterococcus faecalis. The essential oil also possesses notable antifungal activity against Candida albicans, C. krusei, and C. tropicalis, with minimum inhibitory concentrations (MICs) ranging from 0.15–0.3% in liquid media and 15.625–250 μL/mL against various yeasts and fungi. Additionally, it has been reported to inhibit the mushroom pathogen Mycogoneperniciosa, highlighting its potential as a natural antimicrobial and antifungal agent for therapeutic and agricultural applications. ^24,25,26,27 

Hyssopus officinalis essential oil reveal notable antifungal activity. It showed a MIC of approximately 5 μL/mL and minimal fungicidal activity at 15–20 μL/mL against Mycogoneperniciosa. The oil also demonstrated weak-to-moderate activity against Penicillium italicum, Botrytis cinerea, Rhizopus stolonifer, and Phytophthora citrophthora, suggesting potential as a natural food preservative. Moreover, complete inhibition of Aspergillus niger growth was observed at 0.5–1.5% concentrations. In addition, dried leaf extract of H. officinalis showed inhibitory activity against HIV replication, indicating potential antiviral properties. These findings support the traditional medicinal relevance of Zūfā and its potential as a source of natural antifungal and antiviral agents. ^24,28,29.30  

6. Conclusion

Hyssopus officinalis L. (Zūfā) represents an important medicinal plant with a long history of use in Unani medicine, particularly for the management of respiratory disorders such as cough and dyspnoea, as well as gastrointestinal and inflammatory conditions. Contemporary phytochemical investigations have identified a wide range of bioactive constituents, including essential oils, flavonoids, phenolic acids, and terpenoids, which contribute to its diverse pharmacological properties. Evidence from in vitro and in vivo studies supports its antioxidant, antimicrobial, anti-inflammatory, antispasmodic, and cytotoxic activities, while its aromatic characteristics have also established its value in the food, flavour, and fragrance industries.

Despite these promising findings, most available evidence is derived from experimental studies, and well-designed clinical trials remain limited. Standardization of extracts, comprehensive safety and toxicity assessments, and rigorous clinical investigations are necessary to validate its therapeutic efficacy and establish appropriate dosage and quality-control parameters. Integrating traditional Unani knowledge with modern scientific research may facilitate the development of evidence-based herbal formulations and expand the therapeutic potential of H. officinalis in contemporary healthcare.

Acknowledgment: The authors are indebted to the authors and translators of classical Unani texts and authors of research papers whose references have been cited in this manuscript.

Author’s contribution: Mohd Uzair Beg: Conceptualization, Writing- Original draft preparation, Amreen Zehra: Reviewing and Editing. Murad Husain, Reviewing and Editing Md Akhter Hussain Jamali, M.A. Waheed: Reviewing and Editing.

Funding source: Nil.

Conflict of interest: The authors report no conflict of interest in this work.

Ethical approval: Not applicable.

References:

1. Nurzyńska-Wierdak R. Ontogenetic and environmental variability of hyssop (Hyssopus officinalis L.) essential oil composition and activity. Plants. 2026;15(3):487. https://doi.org/10.3390/plants15030487 PMid:41681651 PMCid:PMC12899879
2. Fathiazad F, Hamedeyazdan S. A review on Hyssopus officinalis L.: composition and biological activities. Afr J Pharm Pharmacol. 2011;5(17):1959-1966. https://doi.org/10.5897/AJPP11.527
3. Tahir M, Khushtar M, Fahad M, Rahman A. Phytochemistry and pharmacological profile of traditionally used medicinal plant Hyssop (Hyssopus officinalis L.). J App Pharm Sci, 2018; 8(07): 132-140. https://doi.org/10.7324/JAPS.2018.8721
4. Srivastava A, Awasthi K, Kumar B, Misra A, Srivastava S. Pharmacognostic and pharmacological evaluation of Hyssopus officinalis L. (Lamiaceae) collected from Kashmir Himalayas, India. Pharmacogn J. 2018;10(4):690-693. https://doi.org/10.5530/pj.2018.4.114
5. United States Department of Agriculture (USDA). (n.d.). Retrieved July 12, 2025, from USDA.gov: https://plants.usda.gov/classification/53264
6. Dymock W, Warden CJH, Hooper D. Pharmacographia Indica: A History of the Principal Drugs of Vegetable Origin, Met with in British India. Vol 3. London: Kegan Paul, Trench, Trübner& Co.; 1893. pp-116 https://doi.org/10.5962/bhl.title.10717
7. Mićović T, Katanić Stanković JS. Therapeutic potential of Hyssopus officinalis L. in skin problems - A review. Farmacia. 2024;72(4). https://doi.org/10.31925/farmacia.2024.4.2
8. Unani Pharmacopoeia of India. Part I, Volume II. New Delhi: Ministry of Health & Family Welfare, Department of AYUSH, Government of India; 2007. P 97-98
9. Atazhanova G, Ishmuratova M, Levaya Y, Smagulov M, Lakomkina Y. The genus Hyssopus: traditional use, phytochemicals and pharmacological properties. Plants. 2024;13(12):1683. https://doi.org/10.3390/plants13121683 PMid:38931115 PMCid:PMC11207324
10. Ibn Sina. The Canon of Medicine (Al-Qanun fi al-Tibb). Book II: Simple Drugs. New Delhi: Jamia Hamdard; 1998.
11. Central Council for Research in Unani Medicine. Qarabadeen-e-Sarkari. New Delhi: Ministry of AYUSH; 2006. p. 80.
12. Munshi GN. Makhzan-ul-Mufradat al-maroof Khawas- ul-Advia. New Delhi: Central Council for Research in Unani Medicine, Ministry of AYUSH; 2007. p. 768
13. Ghani HN. Khazain-ul-Advia. New Delhi: Idara Kitab-us-Shifa; n.d. p. 138
14. Besh SB, Mahant M, Patil S, Patel K, Maheshwari RA. Exploring the therapeutic potential of Hyssopus officinalis: A comprehensive review on its pharmacological benefits. J Nat Rem. 2025;25(4):735-47 https://doi.org/10.18311/jnr/2025/45169
15. Hatipoglu G, Sokmen M, Bektas E, Daferera D, Sokmen A, Demir E, Sahin H. Automated and standard extraction of antioxidant phenolic compounds of Hyssopus officinalis L. ssp. angustifolius. Ind Crops Prod, 2013; https://doi.org/10.1016/j.indcrop.2012.07.028
16. Moulodi F, Khezerlou A, Zolfaghari H, Mohamadzadeh A, Alimoradi F. Chemical composition and antioxidant and antimicrobial properties of the essential oil of Hyssopus officinalis L. J Kermanshah Univ Med Sci. 2018;22(4) https://doi.org/10.5812/jkums.85256
17. Dragland S, Senoo H, Wake K, Holte K, Blomhoff R. Several culinary and medicinal herbs are important sources of dietary antioxidants. J Nutr. 2003;133(5):1286-90. https://doi.org/10.1093/jn/133.5.1286 PMid:12730411
18. Steinmetz M, Tognetti P, Mourogue M, Jouglard J, Miller Y. Sur la toxicité de certaineshuilesessentielles du commerce: essence d’hysope et essence de sauge. Plant Med Phytother. 1980;14:34-45. French.
19. Saini A. To explore the ulceroprotective and antioxidant potential of Hyssopus officinalis in ethanol-induced gastric ulcers in rats. Br J Pharm Res. 2012;2(3):197-214. https://doi.org/10.9734/BJPR/2012/1930
20. Soleimani H, Barzegar M, Sahari MA, Naghdi Badi H. An investigation on the antioxidant activities of H. officinalis L. and Echinacea purpurea L. plant extracts in oil model system. J Med Plant. 2011; 10(37):61-72.
21. Hamoud NEH, Maammeri Z. Comprehensive analysis of bioactive compounds in Hyssopus officinalis L. and evaluation of its antioxidant, antibacterial, and anti-Alzheimer properties. J HerbmedPharmacol. 2025;14(1):71-81. https://doi.org/10.34172/jhp.2025.52718
22. Nile SH, Nile AS, Keum YS. Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. 3 Biotech. 2017;7(1):76. https://doi.org/10.1007/s13205-017-0706-9 PMid:28452024 PMCid:PMC5428111
23. Khanna-Chopra R, Semwal VK (2011) Superoxide dismutase and ascorbate peroxidase are constitutively more thermotolerant than other antioxidant enzymes in Chenopodium album. Physiol Mol Biol Plants 17(4):339-346 https://doi.org/10.1007/s12298-011-0083-5 PMid:23573027 PMCid:PMC3550585
24. Sharifi-Rad J, Quispe C, Kumar M, Akram M, Amin M, Iqbal M, et al. Hyssopus Essential Oil: An Update of Its Phytochemistry, Biological Activities, and Safety Profile. Oxid Med Cell Longev. 2022;2022:8442734. https://doi.org/10.1155/2022/8442734 PMid:35069979 PMCid:PMC8776447
25. Proškovcová M, Čonková E, Váczi P, Harčárová M, Malinovská Z. Antibiofilm activity of selected plant essential oils from the Lamiaceae family against Candida albicans clinical isolates. Ann Agric Environ Med. 2021;28(2):260-266. https://doi.org/10.26444/aaem/135892 PMid:34184508
26. Kizil S, Haşimi N, Tolan V, Kilinç E, Karataş H. Chemical composition, antimicrobial and antioxidant activities of hyssop (Hyssopus officinalis L.) essential oil. Not Bot Horti Agrobot Cluj-Napoca. 2010;38(3):99-103. https://doi.org/10.15835/nbha3834788
27. GhasemiPirbalouti A, Gorgij A, Rahimmalek M, Hamedi B. Phytochemical response of hyssop (Hyssopus officinalis L.) to foliar application of jasmonic acid. J Herb Drugs. 2013;4(1):7-14.
28. Raila A, Lugauskas A, Raudonienė V, Railienė M. Different drying technologies and alternation of mycobiota in the raw material of Hyssopus officinalis L. Ann Agric Environ Med. 2009;16(1):93-101.
29. Motiejūnaitė O, Kalėdienė L. Antimicrobial activity of Lamiaceae plant essential oils on Aspergillus niger growth. Biologija. 2003;51:237-242.

30.                 Gollapudi S, Sharma HA, Aggarwal S, Byers LD, Ensley HE, Hinton A. Isolation of a previously unidentified polysaccharide (MAR-10) from Hyssopus officinalis that exhibits strong activity against human immunodeficiency virus type 1. BiochemBiophys Res Commun. 1995;210(1):145-151 https://doi.org/10.1006/bbrc.1995.1639 PMid:7741735