ROLE OF CARBON SOURCE FOR THE PRODUCTION OF L-GLUTAMINASE FROM ASPERGILLUS ORYZAE

  • Sunil Dutt PLNSN Department of Studies in Microbiology, Padmshree Institute of Information Science, Nagarabhavi Circle, Bangalore.
  • M Naveen Research and Development Centre, Bharathiar University, Coimbatore.
  • Yadava Saroj Research and Development Centre, Bharathiar University, Coimbatore.
  • K G Siddalingeshwara Research and Development Centre, Bharathiar University, Coimbatore.

Abstract

Microbial glutaminases are more stable than plant and animal counterparts. In addition to it, they have also been detected in mammalian tissues where they are the major enzymes responsible for catabolic breakdown of glutamine. This study were taken up to the enhance the biosynthesis of L-glutaminase by supplementation of carbon sources were employed in range of 0.25% to 1.0%. The carbon source were supplemented are glucose, fructose and lactose. In case of monosaccharides (fructose) the maximum L-glutaminase production of 494.1 IU was observed at 1% and where as disaccharides like lactose yielded maximum L-glutaminase of 343.5 IU.

Key words: L-glutaminase, submerged fermentation, fructose, Aspergillus oryzae

 

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How to Cite
PLNSN, S., Naveen, M., Saroj, Y., & Siddalingeshwara, K. (2014). ROLE OF CARBON SOURCE FOR THE PRODUCTION OF L-GLUTAMINASE FROM ASPERGILLUS ORYZAE. Journal of Drug Delivery and Therapeutics, 4(5), 193-196. https://doi.org/10.22270/jddt.v4i5.983