ROLE OF CARBON SOURCE FOR THE PRODUCTION OF L-GLUTAMINASE FROM ASPERGILLUS ORYZAE
Microbial glutaminases are more stable than plant and animal counterparts. In addition to it, they have also been detected in mammalian tissues where they are the major enzymes responsible for catabolic breakdown of glutamine. This study were taken up to the enhance the biosynthesis of L-glutaminase by supplementation of carbon sources were employed in range of 0.25% to 1.0%. The carbon source were supplemented are glucose, fructose and lactose. In case of monosaccharides (fructose) the maximum L-glutaminase production of 494.1 IU was observed at 1% and where as disaccharides like lactose yielded maximum L-glutaminase of 343.5 IU.
Key words: L-glutaminase, submerged fermentation, fructose, Aspergillus oryzae
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).