PREPARATION AND CHARACTERIZATION OF ISONIAZID CHITOSAN LOADED NANOPARTICLES
Although the part that we live in, is an age of creative technology and modernization, infectious diseases, like Tuberculosis (TB), still remain to be one of the greatest health challenges worldwide. The objective of this study was to load first line anti-tubercular drug, isoniazid in chitosan nanoparticles in order to enhance its bioavailability and to reduce the dosing frequency. The isoniazid nanoparticles were prepared by Ionic gelation method with addition of suitable polymer (Chitosan) in acidic aqueous solution at various concentrations. The percentage yield obtained for the various formulations was 52-70 % which shows the suitability of ionic gelation technique for preparation of nanoparticles of isoniazid and the entrapment efficiency for the formulations F1-F5 was found to be in the range of 50-70% depending upon the polymer concentrations. It was observed that with an initial increase in polymer i.e. chitosan concentration entrapment efficiency increases, however it dips as concentration of chitosan is increased further because of increased solution viscosity which decreases the loading capacity of nanoparticles. After the SEM studies, it was observed that the formulation F3 nanoparticles was the best being spherical and more regular in shape and size range between 124-220 nm. The F3 isoniazid nanoparticle formulation had a mean particle size of 1.81 nm and zeta potential in (mV) is 52.4. During the in-vitro diffusion studies, it was observed that all the formulations show good control over the release of drug from the nanoparticles matrix. F3 formulation shows 87% drug release in 24 hours. It was observed that F3 follows the Korsmeyer Peppas model (r2 value=0.9864) indicating that the mechanism of drug release from the prepared F3 formulation is non fickian diffusion.
Key words: Isoniazid, Chitosan, Entrapment efficiency, Mean particle size, Zeta potential, Tuberculosis.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).