RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN AND DIFLUPREDNATE IN THEIR COMBINED DOSAGE FORM
AbstractMoxifloxacin is synthetic fluoroquinolone antibiotic agent and Difluprednate is a topical corticosteroid indicated for the treatment of infammation and pain associated with ocular surgery. This combination of drugs will be used to treat optical infections. In RP-HPLC method, a mobile phase of Phosphate Buffer (pH 3.5) : Methanol (35:65 v/v)Â was used to resolve Moxifloxacin and Difluprednate from a mixture. The linearity range obtained for the HPLC method were 25 - 75 Î¼g/ml and 2.5 â€“ 7.5 Î¼g/ml with corresponding correlation coefficient of 0.998 and 0.997, for Moxifloxacin and Difluprednate respectively. Flow rate was set to 1 ml/min and detection was carried out at 244 nm. The method was found to be rapid, accurate and precise. This method was validated according to ICH guidlines.
Download data is not yet available.
330 Views | 370 Downloads
How to Cite
Patel M. RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN AND DIFLUPREDNATE IN THEIR COMBINED DOSAGE FORM. JDDT [Internet]. 14Jul.2014 [cited 27Nov.2022];4(4):60-5. Available from: http://jddtonline.info/index.php/jddt/article/view/913
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).