AN APPROACH ISOLATION,SCREENING AND PRODUCTION OF PROTEASE FROM ASPERGILLUS ORYZAE

  • J Karthic Research and Development Centre Bharathiar University, Coimbatore, India.
  • K G Siddalingeshwara Padmshree Institute of Information Science Nagarabhavi Circle, Bangalore, India.
  • P.L.N.S.N SunilDutt Research and Development Centre Bharathiar University, Coimbatore, India.
  • T Pramod Padmshree Institute of Information Science Nagarabhavi Circle, Bangalore, India.
  • T Vishwanatha Department of Microbiology, Maharani Science College for Women, Bangalore, India.

Abstract

This study investigates the production of extracellular protease synthesis were carried out by using Aspergillusoryzae was evaluated under different fermentation parameters by employing submerged fermentation method. The protease producers detected by the clear zone (casein hydrolysis) around the colony by simple plate assay method. AspergillusoryzaeKS 5 is the potential strain among the fungal isolates. The Proteasesynthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 6.0, temperature 350C and inoculum size 1.0 ml and it showed 1.7 IU.

Key words: Protease, Plate assay, Optimization and Aspergillusoryzae

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1.
Karthic J, Siddalingeshwara K, SunilDutt P, Pramod T, Vishwanatha T. AN APPROACH ISOLATION,SCREENING AND PRODUCTION OF PROTEASE FROM ASPERGILLUS ORYZAE. JDDT [Internet]. 14Mar.2014 [cited 25Sep.2020];4(2):86-9. Available from: http://jddtonline.info/index.php/jddt/article/view/816