AN APPROACH ISOLATION,SCREENING AND PRODUCTION OF PROTEASE FROM ASPERGILLUS ORYZAE
This study investigates the production of extracellular protease synthesis were carried out by using Aspergillusoryzae was evaluated under different fermentation parameters by employing submerged fermentation method. The protease producers detected by the clear zone (casein hydrolysis) around the colony by simple plate assay method. AspergillusoryzaeKS 5 is the potential strain among the fungal isolates. The Proteasesynthesis were increased their yield after the optimization of fermentation parameters. The optimum pH 6.0, temperature 350C and inoculum size 1.0 ml and it showed 1.7 IU.
Key words: Protease, Plate assay, Optimization and Aspergillusoryzae
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