INVESTIGATION ON THE PRODUCTION OF L-GLUTAMINASE FROM PSEUDOMONAS STUTZERI STRAIN UNDER SOLID STATE FERMENTATION USING VARIOUS AGRO RESIDUES

  • R N Athira Dept. of Microbiology, Padmashree Institute of Management and Sciences, Kengeri, Bangalore- 560 060
  • T Elizebeth
  • T Narendra
  • Tanweer Ahmed Sheik
  • Shankar Kumar Gupta
  • Manoj Chaudary
  • K G Siddalingeshwara
  • T Pramod

Abstract

Solid state fermentation was carried out for the production of L-glutaminase by Pseudomonas stutzeri PIMS6 using different agro residues including green gram husk, Bengal gram husk, cattle feed, wheat bran and groundnut oil cake as solid substrates. L-glutaminase has received significant attention in recent years owing to its potential applications in medicine as an anticancer agent, as an efficient anti-retroviral agent and as a biosensor. In food industries it is used as a flavor and aroma enhancing agent. The maximum yield (55.24 U/gds) of L-glutaminase by Pseudomomonas stutzeri PIMS6 was obtained using cattle feed at 75% initial moisture content, initial pH 8.0, supplemented with glucose (1.0%), ammonium sulphate (1.0%),  inoculated with 5% of inoculum and incubated at 37°C for 96 h. Both physico-chemical and nutritional parameters played a significant role in the production of the enzyme L-glutaminase.

Keywords: L-glutaminase, Pseudomonas stutzeri PIMS6, Cattle feed, Solid state fermentation.

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Athira R, Elizebeth T, Narendra T, Sheik T, Gupta S, Chaudary M, Siddalingeshwara K, Pramod T. INVESTIGATION ON THE PRODUCTION OF L-GLUTAMINASE FROM PSEUDOMONAS STUTZERI STRAIN UNDER SOLID STATE FERMENTATION USING VARIOUS AGRO RESIDUES. JDDT [Internet]. 14Mar.2014 [cited 30Nov.2020];4(2):81-5. Available from: http://jddtonline.info/index.php/jddt/article/view/814