Phytochemical screening and In Vitro Antioxidant potential of Memecylon umbellatum Burm leaf extracts

  • Suresh G Killedar Professor and Head, Department of Pharmacognosy, Bharati Vidyapeeth College of Pharmacy, Kolhapur-416013, Maharashtra State, India
  • Sachin S Mali
  • Harinath N More
  • Sameer J Nadaf
  • Sachin S Salunkhe
  • Raviraj S Karade

Abstract

Objective: Different dry extracts of Memecylon umbellatum Burm leaf obtained by various solvents such as petroleum ether, chloroform, ethyl acetate, acetone, methanol and chloroform water (IP) was screened to reap the benefits of its antioxidant and free radical scavenging properties using ascorbic acid as standard antioxidants. Methods: The in vitro free radical scavenging activity was evaluated using diphenyl picryl hydrazyl (DPPH) radical method using various concentrations of dry extract in distilled water (1, 2, 4, 8, 16, 20 μg/ml) against blank with ascorbic acid as a standard in same concentrations. Results: Among the all extracts, Methanol leaf extract has showed higher Antioxidant activity (84.65 ± 0.064 %) having IC50 Value 11.81 ± 0.033 μg/ml at 20 μg/ml. While, IC50 value for ascorbic acid was found to be 8.91 ± 0.084 μg/ml. Conclusion: The results clearly indicate that Methanol leaf extract of Memecylon umbellatum is effective in free radical scavenging. So in future, this may emerge as promising natural herbal source of powerful antioxidant.

Keywords: Memecylon umbellatum, DPPH reagent, Antioxidant activity, Ascorbic acid, IC50.

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Killedar S, Mali S, More H, Nadaf S, Salunkhe S, Karade R. Phytochemical screening and In Vitro Antioxidant potential of Memecylon umbellatum Burm leaf extracts. JDDT [Internet]. 14Mar.2014 [cited 25Feb.2020];4(2):30-5. Available from: http://jddtonline.info/index.php/jddt/article/view/788